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All Journal Prosiding Seminar Biologi BIOMA : Jurnal Biologi Makassar
Rosdarni Rosdarni
STIKES Mandala Waluya Kendari
Agriculture, Biological Sciences & Forestry

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Deteksi MRSA (methicilin resistant Staphylococccus aureus) dengan metode PCR pada pasien ulkus diabetikum Sugireng Sugireng; Rosdarni Rosdarni
Prosiding Seminar Biologi Vol 6 No 1 (2020): PROSIDING SEMINAR NASIONAL BIOLOGI DI ERA PANDEMI COVID-19 (OKTOBER 2020)
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v6i1.15232

Abstract

Salah satu komplikasi diabetes melitus adalah ulkus yaitu terjadi infeksi superfisial pada kulit penderita yang disebabkan oleh bakteri. Staphylococcus aureus merupakan salah satu bakteri yang dapat menginfeksi luka pada penderita ulkus diabetikum. Saat ini S. aureus banyak ditemukan mengalami resisten terhadap antibiotik golongan methicilin atau disebut dengan MRSA (Methicilin-resistant Staphylococcus aureus). Penelitian ini bertujuan untuk mendeteksi adanya gen mecA yang merupakan gen pengkode daerah yang mengalami resistensi pada bakteri S. aureus yang menginfeksi pasien ulkus diabetikum. Penelitian ini dilakukan dengan 3 tahapan yaitu pengambilan sampel swab luka pasien ulkus diabetikum, kultur pada media MSA, proses isolasi DNA, proses PCR dan elektroforesis serta dokumentasi dengan gel doc transluminator. Berdasarkan hasil penelitian pada 8 pasien penderita ulkus diabetikum diperoleh hasil yang menunjukkan bahwa semua sampel dinyatakan sudah terinfeksi Staphylococcus aures yang sudah mengalami resistensi terhadap antibiotik golongan methicilin dengan terbentuknya pita DNA pada ukuran 533 bp.
EFEKTIVITAS ANTIBAKTERI Aglaophenia cupressina Lamoureoux TERHADAP BAKTERI PATOGEN Rosdarni Rosdarni; Ari Tjahyadi
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 6 No. 1 (2021): Bioma, Januari - Juni 2021
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v6i1.11728

Abstract

The level of resistance possessed by most antibiotics is a major problem that must be treated immediately. Several studies on the study of antibiotic resistance have suggested that studies be carried out to find new antimicrobials. One of the antimicrobials that have the potential to inhibit and even have bacteriocidal properties against several resistant pathogens is Aglaophenia cupressina Lamoureoux from the sea. This species has long been used by the people of Linsowu village as a natural anti-toxic agent. This study aims to determine the effect of the concentration of Hydrozoid extract on the growth of pathogenic bacteria that cause infection. This research is an experimental study with a post test only continue design. The research method was carried out in 3 stages, namely the stage of making sample separation in the form of extraction, the stage of testing the extract against 3 different bacteria namely E.coli, Staphylococcus aureus and Streptococcus mutans and measuring the inhibition zone compared to cloranphenicol. The results showed that the extract of Aglaophenia cupressina L. increased the growth of E. coli compared to the two tested bacteria with a zone of 7 mm inhibition based on the level of effectiveness, the extract of Aglaophenia cupressina Lamoureoux is bacteriostatic against E. coli
Co-Authors Ari Tjahyadi Sugireng Sugireng