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Review: Mengenal metode sample pooling untuk pemeriksaan spesimen SARS-CoV-2 Syaiful Rizal
Prosiding Seminar Biologi Vol 7 No 1 (2021): PROSIDING BIOLOGI ACHIEVING THE SUSTAINABLE DEVELOPMENT GOALS WITH BIODIVERSITY I
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v7i1.23642

Abstract

Pandemi COVID-19 yang disebabkan oleh SARS-CoV-2 telah mengakibatkan kerugian hingga kematian di berbagai negara. Peningkatan metode tes untuk skrining COVID-19 merupakan suatu keharusan untuk mencegah penyebaran virus. Ulasan ini bertujuan untuk menjelaskan secara prinsip perbedaan antara metode uji individual dengan metode sample pooling, serta kelebihan dan manfaatnya. Terdapat beberapa uji yang umum digunakan untuk deteksi SARS-CoV-2 antara lain tes rapid antibody, tes rapid antigen, dan tes swab PCR. Tes swab PCR merupakan tes yang direkomendasikan oleh World Health Organization karena sensitivitas dan spesifisitasnya dapat mencapai 100%. Namun uji tersebut juga memiliki beberapa kekurangan yaitu biaya uji mahal, memerlukan fasilitas terstandarisasi, tingginya resiko paparan, serta preparasi dan prosedur pengujian sampel memerlukan sumber daya manusia terlatih. Metode sample pooling merupakan modifikasi dari uji individual karena pada prosesnya spesimen swab suatu individu dicampur dengan spesimen individu yang berbeda secara bersama-sama, kemudian dilakukan ekstraksi RNA dan diuji dengan RT-PCR. Menurut beberapa sumber referensi, hasil RT-PCR dengan metode sample pooling tetap mampu mendeteksi adanya gen SARS-CoV-2 meskipun terjadi pergeseran CT value yang tidak signifikan dengan kondisi tertentu. Metode ini terbukti mampu meningkatkan pengujian SARS-CoV-2 secara masif dan lebih efektif dari segi ekonomi.
CHECKLIST ON FAUNA DIVERSITY GUNUNG HALIMUN SALAK NATIONAL PARK: Cikaniki-Citalahab Agmal Qodri; Ilham Vemandra Utama; Pamungkas Rizki Ferdian; Endah Dwijayanti; Rusdianto Rusdianto; Yohanna Yohanna; Mulyadi Mulyadi; Nanang Supriatna; Rena Tri Hernawati; Fajrin Shidiq; Encilia Encilia; Gloria Animalesto; Pangda Sopha Sushadi; Anang Setyo Budi; Syaiful Rizal; Ujang Nurhaman; Alamsyah Elang Nusa Herlambang; Ayu Savitri Nurinsiyah
ZOO INDONESIA Vol 29, No 2 (2020): Desember 2020
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v29i2.4019

Abstract

The Cikaniki resort is one of the most accessible research stations located in the Gunung Halimun Salak National Park (GHSNP). It is in adjacent with Citalahab village. The Research Center for Biology, Indonesian Institute of Sciences and other institutions have conducted intensive research on the fauna diversity of GHSNP from this station. Here we formulate a checklist on fauna diversity surrounding the Cikaniki Research Station and Citalahab, GHNSP from various sources, i.e. field work, museum collections (Museum Zoologicum Bogoriense), scientific publications, and technical report. The study was conducted from October 2019 until October 2020. The latest field work was conducted from 8-10 October 2019 under the framework of the Jungle Survival and Biological Collection Management 2019 program. In total, 821 fauna species were recorded in Cikaniki-Citalahab areas which comprises of 48 species of Mollusca, five species of Malacostraca, 523 species of Insects, 22 species of Actinopterygii, 63 species of Amphibia and Reptiles, 115 species of Aves and 45 species of Mammals. The diversity contributes 62.1% of the total 1,323 known fauna species in GHSNP. Five number of species were assigned as endangered and three species critical endangered by IUCN. In addition, 123 species were endemic to Java and 34 species protected by Regulation of the Ministry of Environment and Forestry Republic of Indonesia Number P.106/MENLHK/SETJEN/KUM.1/12/2018. The areas of Cikaniki and Citalahab are rich in biodiversity. Although both areas are in close intact with human activity, research and ecotourism, the need of continuously spreading awareness and enforce species and area conservation is inevitable.
CHECKLIST ON FAUNA DIVERSITY GUNUNG HALIMUN SALAK NATIONAL PARK: Cikaniki-Citalahab Agmal Qodri; Ilham Vemandra Utama; Pamungkas Rizki Ferdian; Endah Dwijayanti; Rusdianto Rusdianto; Yohanna Yohanna; Mulyadi Mulyadi; Nanang Supriatna; Rena Tri Hernawati; Fajrin Shidiq; Encilia Encilia; Gloria Animalesto; Pangda Sopha Sushadi; Anang Setyo Budi; Syaiful Rizal; Ujang Nurhaman; Alamsyah Elang Nusa Herlambang; Ayu Savitri Nurinsiyah
ZOO INDONESIA Vol 29, No 2 (2020): Desember 2020
Publisher : Masyarakat Zoologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52508/zi.v29i2.4019

Abstract

The Cikaniki resort is one of the most accessible research stations located in the Gunung Halimun Salak National Park (GHSNP). It is in adjacent with Citalahab village. The Research Center for Biology, Indonesian Institute of Sciences and other institutions have conducted intensive research on the fauna diversity of GHSNP from this station. Here we formulate a checklist on fauna diversity surrounding the Cikaniki Research Station and Citalahab, GHNSP from various sources, i.e. field work, museum collections (Museum Zoologicum Bogoriense), scientific publications, and technical report. The study was conducted from October 2019 until October 2020. The latest field work was conducted from 8-10 October 2019 under the framework of the Jungle Survival and Biological Collection Management 2019 program. In total, 821 fauna species were recorded in Cikaniki-Citalahab areas which comprises of 48 species of Mollusca, five species of Malacostraca, 523 species of Insects, 22 species of Actinopterygii, 63 species of Amphibia and Reptiles, 115 species of Aves and 45 species of Mammals. The diversity contributes 62.1% of the total 1,323 known fauna species in GHSNP. Five number of species were assigned as endangered and three species critical endangered by IUCN. In addition, 123 species were endemic to Java and 34 species protected by Regulation of the Ministry of Environment and Forestry Republic of Indonesia Number P.106/MENLHK/SETJEN/KUM.1/12/2018. The areas of Cikaniki and Citalahab are rich in biodiversity. Although both areas are in close intact with human activity, research and ecotourism, the need of continuously spreading awareness and enforce species and area conservation is inevitable.