Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Hemera Zoa
F. Indrafuri
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Status Meiotik oosit kucing setelah masturasi in vitro pada waktu yang berbeda S. Gustari; S. Budhi; F. Indrafuri; N.W.K Karja
Hemera Zoa Vol. 1 No. 1 (2009): Jurnal Hemera Zoa
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1857.96 KB)

Abstract

The purpose of the research was to determine meiotic status of cat oocytes maturated in vitro at various duration times. Cat ovaries were collected from sexually mature queens after routine ovariohysterectomy. Oocytes were collected from those ovaries by slicing method and only good oocytes were maturated in maturation medium and incubated at 38,5oC in 5% CO2 humidified incubator in various duration. The durations were at 18-20 hours, 22-24 hours and 28-30 hours. At the end of maturation, the oocytes were fixed and stained with acetic orcein. The percentage of oocytes that reached metaphase II for 18-20 hours, 22-24 hours and 28-30 hours maturation were 60% ± 0.04 ; 68% ± 0.31 and 77% ± 0.2, respectively. There were no significant differences (P>0.05) on the percentage of oocytes in reaching the stage of germinal vesicle, anaphase-telophase and methapase II. Keywords : in vitro maturation, cat oocytes and meiotic status of the oocytes.
Co-Authors N.W.K Karja S. Budhi S. Gustari