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Tyas Utami
Jurusan Teknologi Pangan dan Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Jl. Flora, Yogyakarta 55281

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Pengaruh Infeksi Escherichia coli dan Pemberian Lactobacillus plantarum Dad 13 terhadap Mikrobiota Feses Tikus Wistar Bekti Tri Sumaryati; Tyas Utami; Suparmo Suparmo
agriTECH Vol 29, No 4 (2009)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.491 KB) | DOI: 10.22146/agritech.9692

Abstract

The antibacterial activity of Lactobacillus plantarum Dad 13 to pathogenic Escherichia coli was studied in vitro using well diffusion method. The effects of E. coli infection and addition of L. plantarum Dad 13 on fecal microbiota of Wistar rats were studied in-vivo. Rats were fed with standard feed. After five days they were divided into two groups. Rats in the first group were infected with 1010  CFU E. coli enterotoxigenic stabile toxin and rats in the second group were fed with 109  CFU L. plantarum Dad 13 for a week. Samples were taken before and after treatment for enumeration of E. coli, coliform, and lactobacilli in the feses. The results showed that L. plantarum Dad 13 could inhibit pathogenic E. coli indicated by clear zones. Escherichi coli infection didn’t significantly increased the count of E. coli and coliform in the feses, but reduced 0.4 log cycle of the total lactobacilli. Lactobacillus plantarum Dad 13 increased 1.2 log cycle of the total lactobacilli, but did not reduced the count of E. coli and coliform. Infection of E. coli and addition of L. plantarum Dad 13 changed the ratio among fecal microbiota of rats.ABSTRAKTujuan dari penelitian ini adalah mempelajari aktivitas antibakteri Lactobacillus plantarum. Dad 13 terhadap Escher­ ichia coli patogen penyebab diare secara in vitro, dan pengaruh infeksi E. coli patogenik dan pemberian L. plantarum Dad 13 terhadap mikrobiota feses tikus Wistar. Tikus percobaan diberi pakan standar selama lima hari, kemudian dibagi menjadi dua kelompok. Kelompok pertama, tikus diinfeksi dengan 1010 CFU E. coli patogen, sedang tikus pada kelompok kedua diberi L. plantarum Dad 13 sebanyak 109  cfu selama satu minggu. Sebelum dan setelah perlakuan, dilakukan enumerasi E. coli, coliform, dan lactobacilli pada feses tikus. Hasil yang diperoleh menunjukkan bahwa secara in vitro Lactobacillus sp. Dad 13 dapat menghambat pertumbuhan E. coli patogenik. Infeksi dengan 1010 CFU E. coli enterotoksigenik stabil toksin pada tikus Wistar tidak meningkatkan jumlah E. coli dan coliform, namun menu­ runkan jumlah lactobacilli sebesar 0,4 siklus log. Pemberian L.plantaraum Dad 13 tidak dapat menurunkan jumlah E. coli dan coliform, namun dapat meningkatkan jumlah lactobacilli sebesar 1,2 siklus log. Infeksi E. coli dan pemberian L. plantarum Dad 13 mengubah rasio mikrobia dalam feses.
Viabilitas Lactobacillus acidophilus SNP 2 dalam Kapsul dan Aplikasinya dalam Es Krim Andhini Banyuaji; Endang S. Rahayu; Tyas Utami
agriTECH Vol 29, No 4 (2009)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1808.381 KB) | DOI: 10.22146/agritech.9693

Abstract

Due to the health benefit reasons, probiotics have been incorporated into a range of dairy products, including yogurt, cheese, and ice cream. However, the viability of probiotics can decrease during ice cream processing. The reduction of viable probiotics after consumption may also be due to the stomach acid and the presence of bile salt. This research studied the encapsulation of Lactobacillus acidophilus SNP 2 probiotic bacteria using extrusion and emulsion tech­ niques, and their effect on probiotic survival in acid pH, and viability during ice cream processing and storage. Encap­ sulation of probiotic bacteria by extrusion technique produced 3010 µm diameter of rough surface capsules with a lot of cavity. Conversely, emulsion technique produced smooth capsules with average diameter of 54.67 µm. About 94.13% and 94.56 % of viable cells were entrapped in the capsule using extrusion and emulsion encapsulation techniques respectively. Emulsion technique relatively produced better survival of encapsulated cells to low pH at 4 °C and 37 °C than extrusion technique did. Encapsulation techniques effectively protect the encapsulated cells from freezing injury during ice cream processing. There was no significance of reduction of viable cells during ice cream storage at -18°C for 18 weeks. Based on the sensory evaluation, there was no significant difference in organoleptic characteristics among ice cream containing free cell probiotics, ice cream containing encapsulated probiotics and ice cream without probiotics.ABSTRAKKarena manfaatnya bagi kesehatan, probiotik telah diaplikasikan pada berbagai produk olahan susu seperti yogurt, keju, dan es krim. Pada pengolahan es krim, viabilitas bakteri probiotik dapat mengalami penurunan karena proses pengadukan dan pembekuan. Turunnya viabilitas bakteri probiotik juga dapat terjadi karena kondisi asam dan garam empedu setelah produk dikonsumsi. Pada penelitian ini dipelajari teknik enkapsulasi probiotik Lactobacillus acido­ philus SNP2 dengan metoda ekstruksi dan emulsi, pengaruhnya terhadap viabilitas sel pada pH rendah, serta selama pengolahan dan penyimpanan es krim. Hasil penelitian menunjukkan bahwa enkapsulasi dengan metoda ekstruksi menghasilkan kapsul dengan diameter 3010 µm, permukaan kasar dan banyak cekungan, sedang kapsul yang diper­ oleh dengan metoda emulsi mempunyai permukaan halus dengan diameter 54,67 µm. Sel yang terjerat dalam kapsul dengan metoda ekstruksi dan emulsi masing­masing adalah 94,13% dan 94,56%. Ketahanan sel terenkapsulasi dengan metoda emulsi, terhadap pH rendah relatif lebih baik dari pada sel terenkapsulasi dengan metoda ekstruksi. Enkapsu­ lasi sel L. acidophilus SNP 2 efektif melindungi sel dari kerusakan karena pembekuan. Viabilitas sel probiotik dalam es krim selama penyimpanan 18 minggu pada suhu ­18°C relatif tidak mengalami perubahan. Berdasarkan hasil uji sensoris terhadap es krim, tidak terdapat perbedaan yang nyata antara es krim probiotik sel bebas, es krim probiotik sel terenkapsulasi dengan es krim tanpa probiotik.