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Yustinus Marsono

Jurusan Teknologi Pangan dan Hasil Pertanian, Universitas Gadjah Mada, Yogyakarta

Author-ID : 2870952

Agriculture, Biological Sciences & Forestry

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Pengaruh Diet Protein Kedelai terhadap Profil Lipida Tikus Diabetik Induksi Alloxan Ni Wayan Wisaniyasa; Yustinus Marsono; Zuheid Noor
agriTECH Vol 25, No 2 (2005)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Hypolipidemic of soybean protein were evaluated in alloxan-induced diabetic rats. The objectives of this research were to evaluate the effects of soybean protein feeding on total, HDL, LDL cholesterol and triacylglycerol serum level in both normal cholesterol and hypercholesterol alloxan-induced diabetic rats. Twenty-eight male Sprague-Dawley rats (250-300 g) were used and divided into four groups of seven rats. They were (1) Standard-Placebo (STP), (2) StandardAlloxan (STA), Soybean protein-Alloxan (PKA) and (4) Soybean protein- Hypercholesterol-Alloxan (PKHKA) groups. Three groups were diabetic induced by alloxan injection (80 mg/Kg of body weight by intra-muscular injection) and one group was injected with aquabidest (Placebo). PKHKA group was fed hypercholesterol diet for 2 weeks prior to the alloxan injection. STP and STA groups were fed standard diet whereas PKA and PKHKA were fed soybean protein diet for 28 days. Concentration of lipids were determined before injection (0 day ) and 1, 7, 14, 21, and 28 days after injection. It was found that alloxan injection increased serum total and LDL cholesterol and triacylglycerol concentration of STA, PKA and PKHKA rats. In 28 days intervention, soybean protein diet decreased the serum total cholesterol by 12% (PKA) and 33% (PKHKA), LDL cholesterol by 15% (PKA) and 48% (PKHKA); and triacylglycerol by 31% (PKA) and 37% (PKHKA). In addition, the diet increased the serum HDL cholesterol by 3% (PKA) and 8% (PKHKA). On the other hand, standard diets were failed to decreased the serum total cholesterol, LDL cholesterol and triacylglycerol.

Konsentrat Serat Kedelai : Preparasi dan Pengaruhnya terhadap Sifat Fisik dan Kimia Digesta pada Tikus Sri Budi Wahjuningsih; Yustinus Marsono; Zuheid Noor
agriTECH Vol 25, No 2 (2005)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Soy fiber concentrate (SFC) was isolated from soy flour by extraction in alkali solution (pH 9), centrifuged and washed the solid in ethanol 80% then in acetone 33%, and dried. Effects of soy fiber diet on physical and chemical properties of the rat digesta were evaluated. The objective of this study were (I) to determine the composition of the SFC , (2) to evaluate the effect of soy fiber diets on physical properties (water content and weight) and chemical properties ( pH and SCFA) of the caecal digesta in rats. Four groups of jive male Sprague Dawley rats were provided. They were given differ rent diets Le. soybean, low and high soybean fiber and control diets ad libitum for 21 days period. In the end of experiment they were killed, the caecum were cut and the caecal digesta were taken and analyzed for moisture content, weight, pH and SCFA concentrations. It was found that soy fiber concentrate contains 71.52% total dietary fiber (62.56% insoluble fiber and 8.96 soluble fiber). Weight and moisture contents of the digesta were not affected by the soyfiber diets, but the diets decreased the pH of the digesta significantly. The diet also did not affect the molar proportion of the SCFA. SCFA molar ratio of the caecal digesta were: 62-63: 26-27: 10-11 for acetic: propionic: butyric acid

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