Billy Sujatmiko
Bagian Konservasi Gigi, Program Studi Kedokteran Gigi, Universitas Sriwijaya, Palembang, Sumatera Selatan

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Pengaruh lama aplikasi bahan remineralisasi casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACPF) terhadap kekerasan email Miftah Wiryani; Billy Sujatmiko; Rini Bikarindrasari
Majalah Kedokteran Gigi Indonesia Vol 2, No 3 (2016): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.138 KB) | DOI: 10.22146/majkedgiind.11250

Abstract

The effect of application time of CPP-ACPF on enamel hardness. Remineralization process can increase the hardness of enamel due to demineralization process. CPP-ACPF is a material used for enhancing remineralization. However, the application time of CPP-ACPF remain controversial among previous studies. This study was aimed to investigate the effect of various application times of CPP-ACPF on enamel hardness. Thirty premolar teeth were mounted on self cure acrylic resin, and were divided into 5 groups. Demineralization process was performed, and enamel hardness (pre-est) was measured by Vickers Hardness Tester. Remineralization was performed using CPP-ACPF in various application times: 3, 15, 30, 60 minutes, and the control group was only immersed in artificial saliva for 60 minutes, then enamel hardness was measured (posttest). Data were analyzed using paired t-test, one-way ANOVA, and post-hoc Bonferroni. The result of paired t-test showed that all the groups, except the control group, have an increasing enamel hardness that was statistically significant. One-way ANOVA results showed no statistically significant difference among the groups at pretest, but one-way ANOVA results showed statistically significant difference at posttest. Post hoc Bonferroni showed that the significantly difference at posttest occurred between all the treatment groups against the control group, but there were no significant differences between the 3 minutes group to 15 minutes group, between 15 minutes group to 30 minutes group, and between 30 minutes group to 60 minutes group. It was concluded that various application times of CPP-ACPF had an effect on increasing enamel hardness. ABSTRAKProses remineralisasi dapat meningkatkan kekerasan email yang menurun akibat demineralisasi. Bahan remineralisasi yang ideal adalah CPP-ACPF. Terdapat perbedaan lama aplikasi CPP-ACPF dalam berbagai penelitian, selain itu total lama aplikasi yang dibutuhkan CPP-ACPF dalam mekanisme remineralisasi belum diketahui. Penelitian ini dilakukan untuk mengetahui pengaruh lama aplikasi CPP-ACPF terhadap kekerasan email. Tiga puluh mahkota gigi premolar yang ditanam dalam resin akrilik self cure dibagi menjadi lima kelompok, kemudian dilakukan proses demineralisasi. Kekerasan email kemudian diukur menggunakan alat Vickers Hardness Tester. Proses remineralisasi menggunakan CPP-ACPF dilakukan pada masing-masing kelompok dalam berbagai lama aplikasi yaitu 3 menit, 15 menit, 30 menit, 60 menit, serta perendaman dalam saliva buatan selama 60 menit (kontrol). Kekerasan email kemudian diukur kembali (posttest). Data diuji secara statistik menggunakan t-test berpasangan, one-way ANOVA dan post hoc Bonferroni. Hasil paired t-test menunjukkan bahwa seluruh kelompok, kecuali kelompok kontrol, mengalami peningkatan rata-rata kekerasan email secara signifikan. Hasil uji one-way ANOVA pada pretest menunjukkan tidak ada perbedaan kekerasan email yang signifikan. Hasil uji one-way ANOVA pada posttest menunjukkan terdapat perbedaan kekerasan email yang signifikan. Hasil uji post Hoc Bonferroni menunjukkan bahwa terdapat perbedaan kekerasan email yang signifikan pada seluruh kelompok perlakuan terhadap kelompok kontrol, tetapi perbedaan kekerasan email antara kelompok lama aplikasi 3 menit dengan 15 menit, antara lama aplikasi 15 menit dengan 30 menit, serta antara lama aplikasi 30 menit dengan 60 menit tidak menunjukkan perbedaan kekerasan email yang signikan. Kesimpulan penelitian ini adalah berbagai lama aplikasi CPP-ACPF berpengaruh terhadap peningkatan kekerasan email.
Perbandingan kekerasan mikro dentin mahkota setelah aplikasi berbagai bahan bleaching intrakoronal Apriko Merza; Billy Sujatmiko; Rinda Yulianti
Majalah Kedokteran Gigi Indonesia Vol 2, No 3 (2016): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.29 KB) | DOI: 10.22146/majkedgiind.11261

Abstract

Comparing microhardness of dentine crown after application of various intracoronal bleaching agents. The aim of this study is to compare microhardness of dentine crown after treatment with intracoronal bleaching agents. The method of this study was an experimental laboratory. Thirty two extracted human mandibular first premolars without caries, sectioned at 2 mm below Cemento-Enamel Junction were divided into four groups and bleaching agents were sealed into the pulp chambers as follows: group A – 45% carbamide peroxide, group B – 35% hydrogen peroxide, group C – sodium perborate mixed aquadest and group D – aquadest. Access cavities were sealed and then stored in aquadest at 37 °C. Bleaching procedures were performed on days 0, 7, 14 and 21. After 28 days, the teeth were sectioned longitudinally, and planted on acrylyc. Microhardness of dentine crown was measured by vickers microhardness tester. One Way ANOVA and LSD were used to evaluate the effect of intracoronal bleaching agents on microhardness of dentine crown. The results showed that average values of microhardness of dentine crown on group A was 45,04 VHN, group B was 45,42 VHN, group C was 55,22 VHN and group D was 55,63 VHN. In clonclusion, there was si gnificantly different microhardness of dentine crown between group 45% carbamide peroxide and 35% hidrogen peroxide with sodium perborate mixed aquadest, but between group 45% carbamide peroxide with 35% hidrogen peroxide there was no significant difference.ABSTRAKTujuan penelitian ini untuk mengetahui perbandingan kekerasan mikro dentin mahkota setelah aplikasi berbagai bahan bleaching intrakoronal. Jenis penelitian ini merupakan penelitian eksperimental laboratoris. Sebanyak 32 gigi premolar pertama mandibula tanpa karies, telah diekstraksi, dipotong 2 mm di bawah cemento-enamel junction dibagi dalam 4 kelompok dan bahan bleaching dimasukkan ke dalam kamar pulpa, yaitu kelompok A – 45% karbamid peroksida, kelompok B -35% hidrogen peroksida, kelompok C - sodium perborat dikombinasikan dengan aquadest, dan kelompok D – aquadest. Akses kavitas ditutup kemudian disimpan di dalam aquadest dengan suhu 37 °C. Prosedur bleaching dilakukan pada hari ke-0, 7, 14 dan 21. Setelah 28 hari, mahkota gigi dipotong secara longitudinal dan salah satu bagian ditanam di akrilik. Nilai kekerasan mikro dentin mahkota diuji menggunakan Vickers microhardnes tester. One way ANOVA dan uji LSD digunakan untuk mengevaluasi pengaruh berbagai bahan bleaching intrakoronal terhadap kekerasan mikro dentin. Hasil penelitian menunjukkan nilai kekerasan mikro dentin mahkota pada kelompok A sebesar 45,04 VHN, kelompok B sebesar 45,42 VHN, kelompok C sebesar 55,22 VHN dan kelompok D sebesar 55,63 VHN. Kesimpulan dari penelitian ini terdapat perbedaan kekerasan mikro dentin mahkota yang signifikan antara kelompok 45% karbamid peroksida dan 35% hidrogen peroksida dengan sodium perborat dikombinasikan dengan aquadest, sedangkan antara kelompok 45% karbamid peroksida dengan 35% hidrogen peroksida tidak terdapat perbedaan yang signifikan.
Perbandingan tingkat kebocoran mikro resin komposit bulk-filldengan teknik penumpatan oblique incremental dan bulk Dimas Puja Permana; Billy Sujatmiko; Rinda Yulianti
Majalah Kedokteran Gigi Indonesia Vol 2, No 3 (2016): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (905.741 KB) | DOI: 10.22146/majkedgiind.11668

Abstract

Micoleakage comparison of bulk-fillcomposite beetwen oblique incremental and bulk placement techniques. Resin composite bulk-fill was a new type of resin composite that speed up application process of composite. The concept of bulk-fill composite allows composite to fill at a depth of 4 mm and minimizes polymerization shrinkage. This study aims to determine the comparison of placement techniques (oblique incremental/bulk) of bulk-fill composite on microleakage in class I preparations. Thirty two human maxillary premolar were stored in distilled water, then Class I preparations were made with the depth of the cavity which was 4 mm (3 x 3 x 4). The teeth were randomly divided into two groups, group 1 uses oblique incremental placement technique and group 2 with bulk placement technique. Samples were stored in an incubator at a temperature of 37 °C for 24 hours, then it was thermocycled manually, 100 cycles at temperature between 5 °C and 55 °C. Microleakage was measured using a digital microscope with a 100 X magnification in millimeters using a microscope micrometer calibration ruler with 0,1 mm level of accuracy after immersion in 0,3% methylene blue and sectioned using separating disc. The result of this study revealed that in group 1 microleakage range was 1.0 mm - 2.7 mm with an average 1.625 mm, and in group 2 microleakage range was 3.6 mm - 4.0 mm with an average of 3.763 mm. The data were analyzed using T-test. The analysis showed a significant difference between two groups (p <0.05). The conclusion of this study was bulk-fill composite in class I cavities with oblique incremental placement technique produces less microleakage than bulk placement technique. ABSTRAKResin komposit bulk-fill adalah resin komposit yang dirancang untuk mempercepat proses aplikasi resin komposit. Konsep bulk-fill memungkinkan resin komposit ditumpat sekaligus 4 mm dan mengalami pengerutan polimerisasi minimal. Penelitian ini bertujuan mengetahui efek teknik penumpatan (oblique incremental/bulk) pada tingkat kebocoran mikro resin komposit bulk-fill. Sampel berjumlah 32 gigi premolar rahang atas disimpan di dalam aquades, dipreparasi kavitas kelas I berbentuk persegi, kedalaman kavitas 4 mm (3 x 3 x 4). Sampel dibagi dalam dua kelompok, kelompok 1 restorasi teknik oblique incremental dan kelompok 2 teknik bulk. Sampel kemudian disimpan di dalam inkubator pada suhu 37 °C selama 24 jam, kemudian dilakukan prosedur thermocycling manual 100 putaran pada suhu 5 °C dan 55 °C. Kebocoran mikro diukur menggunakan digital microscope pembesaran 100 x dalam satuan milimeter menggunakan penggaris mikrometer mikroskop dengan ketelitian 0,1 mm setelah sampel direndam dalam larutan methylene blue 0,3% selama 24 jam dan dipotong pada pertengahan restorasi menggunakan separating disc. Hasil pengukuran tingkat kebocoran mikro menunjukkan pada kelompok 1 nilai kebocoran mikro yang terjadi berkisar 1,0 mm – 2,7 mm dengan rata-rata 1,625 mm, pada kelompok 2 kebocoran mikro yang terjadi berkisar 3,6 mm – 4,0 mm dengan rata-rata 3,763 mm. Data di analisis menggunakan uji T-test. Hasil analisis menunjukkan terdapat perbedaan yang signifikan antara kedua kelompok (p < 0,05). Kesimpulan penelitian ini adalah restorasi resin komposit bulk-fill pada kavitas kelas I dengan teknik oblique incremental menghasilkan tingkat kebocoran mikro yang lebih kecil dibandingkan dengan teknik bulk.
Perbandingan efektivitas berbagai obat kumur terhadap kadar Imunoglobulin A pada saliva penderita kariesComparison of mouthwashes effectiveness to the level of salivary immunoglobulin A in caries patients Siti Rusdiana Puspa Dewi; Abu Bakar Lutfi; Veronita Veronita; Fahma Alfarizqy Amarel; Tissa Indira; Debby Handayani Harahap; Theodorus Theodorus; Billy Sujatmiko
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 30, No 2 (2018): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.893 KB) | DOI: 10.24198/jkg.v30i2.17063

Abstract

Pendahuluan: Berbagai obat kumur banyak ditemukan di pasaran diantaranya klorheksidin, povidon iodin, cethylpiridinium chloride (CPC), dan oxygenating agent yang telah terbukti memiliki sifat antibakteri. Di dalam rongga mulut, Imunoglobulin A (IgA) pada saliva berfungsi sebagai pertahanan lokal melawan patogen mulut, sehingga mampu mencegah perkembangan karies. Tujuan dari penelitian ini adalah mengetahui perbandingan efektivitas berbagai obat kumur terhadap kadar Imunoglobulin A pada saliva penderita karies. Metode: Penelitian yang dilakukan penelitian Randomized controlled trial (Uji klinik acak berpembanding), paralel dalam bentuk single blind. Populasi dalam penelitian ini adalah 100 mahasiswa Program Studi Kedokteran Gigi Universitas Sriwijaya yang menderita karies. Responden dipilih berdasarkan kriteria akan dibagi menjadi 5 kelompok secara acak, yaitu kelompok 1 berkumur dengan akuades (sebagai kelompok kontrol), kelompok 2 berkumur menggunakan klorheksidin 0,2%, kelompok 3 berkumur dengan povidon iodin 1%, kelompok 4 berkumur dengan cetylpyridinium chloride 0,05%, dan kelompok 5 berkumur dengan dengan oxygenating agent 0,4%. Data dianalisa dengan uji t berpasangan dan uji t tidak berpasangan. Hasil: Hasil uji t tidak berpasangan dilihat bahwa semua kelompok pengguna obat kumur efektif dalam meningkatkan kadar IgA pada saliva penderita karies secara signifikan (p< 0,05) dibandingkan dengan kelompok kontrol. Peningkatan kadar IgA tertinggi adalah pada kelompok yang berkumur dengan CPC, diikuti dengan berkumur klorheksidin, povidon iodin, dan  oxigenating agent. Simpulan: Obat kumur klorheksidin, povidon iodin, cethylpiridinium chloride, dan oxygenating agent efektif dapat meningkatkan kadar IgA pada saliva penderita karies.Kata kunci: Imunoglobulin A, karies, obat kumur. ABSTRACTIntroduction: Various mouthwashes are circulating in the markets including chlorhexidine, povidone iodine, cethylpiridinium chloride (CPC), and oxygenating agent that have been shown to have antibacterial properties. In the oral cavity, salivary immunoglobulin A (IgA) serves as local defense againts oral pathogents, thus preventing caries development. This study was aimed to compared various mouthwashes on the level of salivary IgA in caries patients. Methods: This study was randomized controlled trial, paralleled in the form of single blind. Population of this study was as much as 100 students of Dentistry Study Program of Sriwijaya University with dental caries. Respondents were divided into 5 groups; group 1 was gargled with aquadest (control group), group 2 with 0.2% chlorhexidine, group 3 with 1% povidone iodine, group 4 with 0.05% CPC, and group 5 with 0.4% oxygenating agent. Data were analyzed by the paired t-test and independent t-test. Result: The results of unpaired t-test showed that all mouthwashes were effective in increasing the IgA levels of caries patients significantly (p < 0.05) compared to the control group. The highest IgA level increase was was found in the CPC group, followed by chlorhexidine, povidone iodine, and oxygenating agent. Conclusion: Chlorhexidine, povidone iodine, cethylpiridinium chloride (CPC), and oxygenating agent contained mouthwashes were able to increase the salivary IgA level of caries patients. Keywords: Immunoglobulin A, caries, mouthwash.
Perbandingan Aktivitas Antibakteri Ekstrak Curcuma zedoaria dan Bahan Irigasi Natrium Hipoklorit 2.5% terhadap Enterococcus faecalis Martha Mozartha; Prisisilia Silvia; Billy Sujatmiko
Jurnal Material Kedokteran Gigi Vol 8 No 1 (2019): JMKG Vol 8 No 1 Maret 2019
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v8i1.330

Abstract

Sodium hypochlorite (NaOCl) is a powerful disinfectant used in root canal therapy, yet it has some disadvantages. There are growing interests of using extracts of natural materials containing antibacterial secondary metabolite compounds as an alternative of irrigation solution. Aim, to compare the antibacterial activity of white turmeric extracts and 2,5% sodium hypochlorite irrigant against Enterococcus faecalis. Methods, this was a quasi experimental study. White turmeric extracts at the concentrations of 75%, 50%, 25% and 10% were used as experimental groups, while 2,5% NaOCl and distilled water were the control groups. Suspensions of Enterococcus faecalis were prepared to a 0.5 Mc Farland standard and applied onto petri plates containing Muller Hinton Agar. Six wells were created in each plate, and filled with 20 mL of the test solution. Plates were incubated at 37 ° C for 24 hours. The antibacterial activity was evaluated by measuring the clear zone around the well. Data were analyzed by Kruskall Wallis and continued by Mann U Whitney. Results, the largest growth inhibition zones were produced when the test bacteria were in contact with 2,5% NaOCl (15,0233 mm). White turmeric extract 75% concentration had weak antibacterial effect, while white turmeric extract 50%, 25%, 10% concentration, and distilled water did not has antibacterial properties against Enterococcus faecalis. Conclusion, white turmeric extract 75% concentration can inhibit the growth of Enterococcus faecalis with weak antibacterial activity.