Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Indonesian Journal of Biotechnology Jurnal Nasional Teknologi Terapan
Medania Purwaningrum
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Immunology & microbiology Materials Science & Nanotechnology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method Diana Savitri; Irhamna Putri; Warih Pulung Nugrahani; Medania Purwaningrum; Aris Haryanto
Indonesian Journal of Biotechnology Vol 26, No 1 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.54611

Abstract

Sex identification of endangered and protected birds in captivity is very important for conservation programs. Half of the world’s bird species are monomorphic, where male and female are difficult to distinguished morphologically, including cockatoos. Sex identification using molecular bird sexing is more accurate and applicable because it directly targets the sex chromosomes. The purpose of this study was to determine the sex of Sulphur‐crested cockatoo (Cacatua galerita) by detecting differences in the intron size of the chromodomain helicase DNA‐binding 1 (CHD1) gene on the Z and W chromosomes by polymerase chain reaction (PCR) method and to compare of plucked feathers and blood samples as DNA sources for molecular bird sexing. DNA was extracted from feather and blood samples from four C. galerita. Extracted DNA was amplified on the CHD1 gene by PCR method with P2, MP, and NP primers, which were visualized using agarose gel 1.5% under UV transilluminator with a wavelength of 280 nm. The resulting PCR product was detected at 392 bp for the CHD1 Z gene segment and 297 bp for CHD1 W gene segments, where males showed a single DNA band (ZZ) and females showed a double DNA band (ZW). Four C. galerita were 100% successfully determined, consisting of one female and three males. Electrophoresis results showed DNA bands from blood samples were thicker and brighter than DNA bands from feather samples.
Deteksi Molekuler Gen Fusion (F) dan Analisis Perbandingan Beberapa Enzim Restriksi sebagai Penentu Patotipe Virus Newcastle Disease Medania Purwaningrum; Verawati Verawati; Aris Haryanto
Jurnal Nasional Teknologi Terapan (JNTT) Vol 2, No 3 (2018): NOVEMBER
Publisher : Penelitian dan Pengabdian Kepada Masyarakat Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.57 KB) | DOI: 10.22146/jntt.44935

Abstract

Newcastle disease (ND) is a contagious viral disease caused by Avian Paramyxovirus Serotype-1 (APMV-1). This viral infection is responsible for devastating outbreak by attacking nerve, respiration, and also digestive system. This disease often followed with decreasing of eggs production and also responsible for economic losses in the poultry industries around the globe. The main goal was to differentiate virulent or avirulent strain of ND virus from F gene, which is the virulent marker of ND virus, by Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Restrion Enzyme Analysis using BamH1, Hin 1l, and Apa 1. Ten ND virus samples came from Animal Disease Investigation Center (ADIC) Wates virus collection, collected from field case in 2012-2013. Newcastle disease virus was collected by extraction from the samples. The RNA product of extraction were used as a template for amplification in RT-PCR. The target of RT-PCR amplification was F gene. The results indicated positive reaction due to existing of DNA fragment band in size of 767 bp. RT-PCR and Restrion Enzyme Analysis can be used as tool to determine the pathotype of ND virus showed different restriction visualized by gel agarose electrophoresis.
Co-Authors Aris Haryanto Diana Savitri Irhamna Putri Verawati Verawati Warih Pulung Nugrahani