Anggi Wahyu Nur Cahyani
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EFEK ANTIBAKTERI SUPERNATAN JUS ANGGUR MERAH YANG DIISOLASI DENGAN KECEPATAN SENTRIFUGASI 12.000 rpm TERHADAP PERTUMBUHAN S. MUTANS Dini Rachmawati; Sumarno .; Anggi Wahyu Nur Cahyani
ODONTO : Dental Journal Vol 3, No 2 (2016): December 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.3.2.81-87

Abstract

Background: Streptococcus mutans (S.mutans) is a gram-positive bacteria in oral cavity which produced acid as the common cause of dental caries. In order to prevent the growth or kill Streptococcus mutans, antibacterial is required. Red grape (Vitis vinifera) is suspected to contain antibacterial.Method: An experimental study using the post test only control group design is carried out to Streptococcus mutans with tube dilution method and streaking on the Brain Heart Infusion. A range of concentration from 40%, 50%, 60%, 70%, 80%, 90%, 98%. The control group are group of bacteria and red grape juice control.Result: The analysis of Minimum Inhibitor Bactericidal (MIC) is show a difference absorbent value as the given concentration (Anova, p<0,05). The correlation test show is strong reciprocal association between concentration of res grape juice and absorbent value (r=-0,978; p< 0,05). The analysis of Minimum Bactericidal Concentration (MBC) is shows difference of the number of colonies as given concentration (Anova, p<0,05). The correlation reveals a very strong reciprocal association between concentration of red grape juice and the number of Streptococcus mutans colonies growth (r=-0,925; p< 0,05).Conclusion: The conclusion is supernatant of red grape juice has effect the growth as antibacterial agent on Streptococcus mutans in vitro, with MIC at concentration 60% and MBC at concentration 80%
EFEK ANTIBAKTERI SUPERNATAN JUS ANGGUR MERAH YANG DIISOLASI DENGAN KECEPATAN SENTRIFUGASI 12.000 rpm TERHADAP PERTUMBUHAN S. MUTANS Dini Rachmawati; Sumarno .; Anggi Wahyu Nur Cahyani
Odonto : Dental Journal Vol 3, No 2 (2016): December 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.743 KB) | DOI: 10.30659/odj.3.2.81-87

Abstract

Background: Streptococcus mutans (S.mutans) is a gram-positive bacteria in oral cavity which produced acid as the common cause of dental caries. In order to prevent the growth or kill Streptococcus mutans, antibacterial is required. Red grape (Vitis vinifera) is suspected to contain antibacterial.Method: An experimental study using the post test only control group design is carried out to Streptococcus mutans with tube dilution method and streaking on the Brain Heart Infusion. A range of concentration from 40%, 50%, 60%, 70%, 80%, 90%, 98%. The control group are group of bacteria and red grape juice control.Result: The analysis of Minimum Inhibitor Bactericidal (MIC) is show a difference absorbent value as the given concentration (Anova, p<0,05). The correlation test show is strong reciprocal association between concentration of res grape juice and absorbent value (r=-0,978; p< 0,05). The analysis of Minimum Bactericidal Concentration (MBC) is shows difference of the number of colonies as given concentration (Anova, p<0,05). The correlation reveals a very strong reciprocal association between concentration of red grape juice and the number of Streptococcus mutans colonies growth (r=-0,925; p< 0,05).Conclusion: The conclusion is supernatant of red grape juice has effect the growth as antibacterial agent on Streptococcus mutans in vitro, with MIC at concentration 60% and MBC at concentration 80%