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All Journal Indonesian Journal of Cancer Chemoprevention Biology, Medicine, & Natural Product Chemistry
Chanif Mahdi
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Brawijaya
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

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Kidney Evaluation in Hyperuricemia Rats Treated with Green Tea Leaves (Camellia sinensis L.) Extract Putranty Widha Nugraheni; Chanif Mahdi
Biology, Medicine, & Natural Product Chemistry Vol 11, No 1 (2022)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2022.111.17-26

Abstract

Uric acid is an oxidation product of the xanthine oxidase enzyme found in extracellular fluid, and when it exceeds, uric acid will build up and cause hyperuricemia. TNF-α is released by epithelial cells and mesangial cells when inflammation occurs and causes apoptosis in epithelial cells, causing damage to kidney structures and initiating acute kidney poisoning. Green tea extract (Camellia sinensis L.) contains many antioxidants, especially flavonoids with potent antioxidant properties such as lipid peroxidase and free radical absorbers, inhibiting xanthine oxidase. This study expresses the potential of green tea extract on kidney repair caused by HUA. Twenty-four male albino rats (175-225 g) of Wistar strain being fed a high purine diet in 60 consecutive days and divided into six groups randomly, I: negative control, II: positive control, III: allopurinol, IV: green tea extract 150mg of body weight, V: green tea extract 300mg of body weight, and VI: green tea extract 600mg of body weight. Treatment was done for 14 days and measured by total creatinine levels, malondialdehyde levels, and kidney histopathology. The statistical analysis using One Way ANOVA and Post Hoc Tukey analysis by SPSS 23.0 proved that green tea extract with a dose of 600 mg/kg of body weight green could lower levels malondialdehyde of the kidney as much as 58.85% (p<0.01), decreased creatinine level by 24.5% (p>0.05), and improved kidney histopathology. This study proved that green tea extract is a promising alternative for hyperuricemia while improving kidney tissues and lowering malondialdehyde and creatinine levels.
Effect of Goat Milk Yogurt for Prevention Hypercholesterolamic in Animal Model Rat (Rattus norvegius) Based on the Expression of Inducible Nitric Oxide Synthase (INOS) and a description of Liver Histopathology Chanif Mahdi; Sukarno Hariyanto; Masdiana C. Pandaga
Indonesian Journal of Cancer Chemoprevention Vol 7, No 2 (2016)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev7iss2pp38-45

Abstract

The pattern of high consumption of fat can lead to hypercholesterolemia were accompanied by increased levels of LDL which resulted in LDL oxidation, accompanied by an inflammatory reaction that can lead to liver tissue damage resulting in increased expression of inducible Nitric Oxide Synthase (INOS) and changes in liver histopathology. The purpose of this study was to investigate the influence of goat milk yogurt supplementation on the expression of INOS and liver histopathology picture. This study used 20 male rats, Wistar strain aged 10-12 weeks, weighing 150-200g. Giving goat milk yogurt for 42 days with a dose of 300mg/kg, 600mg/kg, and 900mg/kg and giving dietary hypercholesterolemia by administering poached quail egg yolk, lard, and cholic acid by the stomach sonde for 14 days. Parameters measured were the expression of INOS and liver histopathology picture. The analysis used in this study is the One Way Analysis Of Variance (ANOVA) followed by Tukey test 5%. The results showed that administration of goat milk yogurt as a precaution hypercholesterolemia at a dose of 300mg/kg, 600mg/kg and 900mg/kg give a highly significant effect (p <0.01) on the expression of INOS (p <0.01).The overall Result of each treatment were 0.47 ±0.03; 6.72 ± 0.11; 2.40 ±0.16;1.11 ±0.07and 0.66 ±0.19  Prevention with a dose of 900 mg/kg body weight is the most effective dose inhibits the expression of INOS.  Prevention with a dose of 900 mg/kg showed that the histopathological picture of approaching normal circumstances. The conclusion of the study is the provision of goat milk yogurt as a preventive action with a dose of 900 mg/kg of the most effective to inhibit the expression of INOS and maintain normal liver histology in rats given diets hypercholesterolemia.Keywords: Hypercholesterolemia, Expression of Inducible Nitric Oxide Synthase (INOS), goat's milk yogurt, liver histopathology
Co-Authors Masdiana C. Pandaga Putranty Widha Nugraheni Sukarno Hariyanto