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Budi Mulyaningsih
Gadjah Mada University (UGM)
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Larvicidal Activity of A Mixture of Cashew Nut Shell Liquid and Water-Soluble Extract of Soap Nut Fruit (Sapindus rarak DC.) Against 3rd Instar Larvae of Aedes aegypti Glory Resia Raraswati; Sudarsono Sudarsono; Budi Mulyaningsih
Biology, Medicine, & Natural Product Chemistry Vol 3, No 2 (2014)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.765 KB) | DOI: 10.14421/biomedich.2014.32.53-57

Abstract

Cashew Nut Shell Liquid (CNSL) which has been known as a waste of processing cashew fruits which is contain phenolic compounds have activity as larvicides. Cashew nut shell liquid is not soluble in the water where the larvae grow. Cashew nut shell liquid mixed with water-soluble extract of soapnut fruit which serves as a natural surfactant that can emulsify oil in water. The test subjects were larvae of Aedes aegypti third instar. Test subjects were divided into treatment group and control group. In the treatment group, test subjects were  the mixture of CNSL and Ethylacetat Soluble Extract (ESE) in tap water. The Larvae mortality observations were done 24 hours after the treatment. LC50 and LC90 as final test data were analyzed using probit analysis. Extract constituents   of CNSL and Water Soluble Extract of SoapNut fruit (WSEoSN) were  investigated using Thin Layer Chromatography (TLC) method. The effect of CNSL as larvicides against third instar larvae of Ae. aegypti with were LC50 of 14,12 ppm, while the LC90 of 24,85 ppm.
Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography Rahmawati Ekaputri; Sudarsono Sudarsono; Budi Mulyaningsih
Biology, Medicine, & Natural Product Chemistry Vol 3, No 1 (2014)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.324 KB) | DOI: 10.14421/biomedich.2014.31.31-33

Abstract

Background: Vinca rosea is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of Vinca rosea was selected to investigation its larvicidal activity against the 3rd instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) Aedes aegypti.  Five concentrations of Vinca fruit extract were tested against the 3rd instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC50 anf LC90) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds  of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC50 and LC90 values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.
Co-Authors Glory Resia Raraswati Rahmawati Ekaputri Sudarsono Sudarsono