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Maman Turjaman
Badan Litbang Kehutanan Gunung Batu, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Social Sciences

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Surface Sterilization to Isolate Fungal Root Endophytes of Meranti Tembaga (Shorea leprosula Miq.) in Dramaga Experimental Forest Safinah S. Hakim S Hakim; Sri Wilarso Budi; Maman Turjaman
Jurnal Silvikultur Tropika Vol. 5 No. 1 (2014): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.5.1.%p

Abstract

Root fungal endophyte were isolated from seedling of Meranti Tembaga (Shorea leprosula Miq.) taken from Dramaga experimental Forest, Bogor with five difference surface sterilization methods. Recommended surface sterilization to isolate root fungal endophyte was performed by following immersion process : 96% ethanol for 1 min, 20% H2O2 for 3 min, 96% ethanol for 0.5 min, and rinse with sterile water for 5 min. Endophyte frequency was low (6-11%) and four morphological types of fungi were found during this research.
Co-Authors Safinah S. Hakim S Hakim Sri Wilarso Budi