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TRIGLYCERIDE COMPOSITION OF SIXTEEN STRAINS OF MARINE DIATOM Lily M.G. Panggabean; Abdullah Rasyid; Zarrah Duniani; Yana Meliana; Indah Kurniasih
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 1 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.952 KB) | DOI: 10.29244/jitkt.v5i1.7762

Abstract

Trigliceride or triacylglicerol (TAG) composition in crude oil of sixteen strain of marine diatom has been detected by spectra analyses on an Electrospray - Ion Trap – Mass Spectrometry (ESI-IT-MS) HCT Bruker-Daltonic GmbH instrument with AgNO3 used as coordination ionization agent. Biomass samples of each microalga strain were taken from early and late stationary cultures in f/2 enriched seawater and algal oils were extracted according to Bligh and Dyer. Results from spectra analysis showed that P-Pt-P (C16:0-C16:1-C16:0) were distinguished in TAG from diatom strains Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.1, Thalasiossira sp.2, Thalasiossira sp.3, Navicula sp. 1, Navicula sp. 2, Navicula sp. 3, Navicula sp. 4, Nitzschia sp. 2 and Amphora sp. In contrast, TAGs in Melosira sp. included P-P-P (C16:0-C16:0-C16:0) and P-P-O (C16:0-C16:0-C18:1) were identified. TAGs from Chaetoceros sp. were the most varies among samples, i.e. P-Pt-P (C16:0-C16:1-C16:0), A-P-M (C20:4-C16:0-C14:0), P-Pt-Lt (C16:0-C16:1-C18:3), P-Pt-A (C16:0-C16:1-C20:4), D-P-P (C22:6-C16:0-C16:0), A-Ln-P (C20:4-C18:2-C16:0). Various TAGs were also detected in Nitzschia sp.2, i.e. P-Pt-M (C16:0-C16:1-C14:0), P-Pt-P (C16:0-C16:1-C16:0), P-Pt-S (C16:0-C16:1-C18:0), P-Pt-A (C16:0-C16:1-C20:4). TAGs composition in Skeletonema strains that similar to those in Nitzschia sp.1 has longer carbon, i.e. P-P-O (C16:0-C16:0-C18:1), P-O-O (C16:0-C18:1-C18:1) and O-O-O (C18:1-C18:1-C18:1). TAGs with longer carbon chain and more double bond including highly unsaturated fatty acid C20:4 were increased with culture age in diatoms Chaetoceros sp.1, Chaetoceros sp.2, Thalasiossira sp.2, Navicula sp.1 and Nitzschia sp. 2.Keywords: diatom, TAG, ESI-IT-MS, f/2, early and late stationary
IDENTIFICATION OF SECONDARY METABOLITES COMPOUNDS, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES ON THE METHANOL EXTRACT OF SEA CUCUMBER Stichopus hermanii Abdullah Rasyid
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 4 No. 2 (2012): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.307 KB) | DOI: 10.29244/jitkt.v4i2.7799

Abstract

Identification of secondary metabolites, antibacterial and antioxidant activities assay of methanol extract of sea cucumber Stichopus hermanii were conducted from May to July 2011 at the Natural Products Laboratory, Research Center for Oceanography, Indonesian Institute of Sciences. Sea cucumber used in this study comes from South Lampung waters. Objective of the study was to get information of secondary metabolites, antibacterial and antioxidant activities of methanol extract of sea cucumber S. hermanii. The extraction method which used in this experiment was the maceration method using methanol solvent. Identification of secondary metabolites was performed through observing reaction of colors, precipitation, and foaming. Antibacterial and antioxidant activities of methanol extract from sea cucumber were tested using agar diffusion method and reducing of free radicals 1.1-Diphenyl-2-Picrylhydrazyl (DPPH) respectively. The results showed that secondary metabolites identified in the methanol extract of sea cucumber S. hermanii were saponin and steroids. Both secondary metabolites had antibacterial activities against Staphylococcus aureus, Vibrio eltor and Bacilus subtilis. The analysis of antioxidant activity showed that the IC50 value of methanol extract of sea cucumber S. hermanii was about 65.08 ppm. It indicated that S. hermanii is having potency as antibacterial and antioxidant. Keywords: antibacterial, antioxidants, sea cucumber Stichopus hermanii, secondary metabolites
Penerapan Media Edukatif untuk Meningkatkan Kesadaran Pengelolaan Sampah pada Siswa SD Negeri Jambewangi, Kecamatan Pakis, Kabupaten Magelang Wisnu Satrio Amaanulaah Akmal; Abdullah Rasyid; Lailani Amalia; Nofithania Syawalaila Ayusandrina; Wakhidatul Arifah; Sulistyowati Sulistyowati; Khusna Zulfatul Arifah; Muhammad Herfa Setiawan; Galang Adri Saputra; Verdhi Adnan Qur Rachim; Olivia Wardhani
Manfaat : Jurnal Pengabdian Pada Masyarakat Indonesia Vol. 2 No. 3 (2025): Agustus : Manfaat : Jurnal Pengabdian Pada Masyarakat Indonesia
Publisher : Asosiasi Riset Ilmu Tanaman Dan Hewan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/manfaat.v2i3.443

Abstract

Schools are not only institutions for the transfer of knowledge but also environments that play a crucial role in shaping students’ behavior and instilling positive habits, one of which is environmental care. One form of environmental concern that can be cultivated from an early age is the discipline of disposing of waste properly. However, SD Negeri Jambewangi, located in Dusun Pesantren, has not yet fully implemented effective waste sorting practices. Waste disposal is still carried out without distinguishing between organic and inorganic waste, which may have negative impacts on the school environment. To address this issue, community service activities were carried out by KKN students from Universitas Tidar through outreach programs and the distribution of sorted waste bins. The socialization activity applied the Extend Parallel Process Model (EPPM) communication strategy using a Severity Model approach, which emphasized the dangers and consequences of improper waste management practices. During the sessions, students were encouraged to discuss and identify various types of waste, as well as the potential environmental and health impacts associated with them. Direct practice was also provided by encouraging students to actively participate in sorting waste before disposal into the designated bins. The implementation of this program showed a positive impact, as students demonstrated an increased understanding of waste management and began to practice sorting waste more consistently. The provision of separate bins for organic and inorganic waste further facilitated this new habit. It is expected that the discipline of sorting and properly disposing of waste will continue to be applied by students, teachers, and the entire school community. Such continuous practices can foster collective awareness, contribute to maintaining environmental cleanliness, and provide long-term benefits not only for the school but also for the surrounding community.