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All Journal Jurnal Teknologi Dan Industri Pangan Journal of Food and Pharmaceutical Science Journal of Food and Pharmaceutical Sciences
Jenny Elisabeth
Faculty of Agriculture, Universitas Sumatera Utara, Medan, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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The specificity of Several Kinds Lipases on n-3 Polyunsaturated Fatty Acids Jenny Elisabeth; T Yuliani; P M Tambunan; J M Purba
Jurnal Teknologi dan Industri Pangan Vol. 12 No. 1 (2001): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Several lipases from microbial and plant, i.e Rhizomucor miehei, Pseudomonas sp., Candida antartica, rice bran, and Carica papaya latex (CPL) were examined for synthesis of omega-3 (n-3) PUFA-rich glyceride by hydrolysis and acidolysis reaction. Tuna oil was used in hydrolysis reaction, whereas tuna and palm oils were used as source of triglyceride (TAG) molecules and n-3 PUFA concentrate from tuna oil as source of EPA and DHA in acidolysis reaction.For hydrolysis reaction, the rice bran and CPL lipases showed the lowest hydrolytic activity of the tuna oil, whereas the R. miehei lipase showed the highest hydrolytic activity but was unable to hydrolyze EPA and DHA. On the contrary, the C. antartica and Pseudomonas sp. lipases acted stronger on hydrolysis of DHA ester bond than EPA.For acidolysis reaction, all the lipases showed ability to incorporate n-3 PUFA into tuna and palm oils. C. antartica lipase had the maximum DHA incorporation into tuna and palm oils, rice bran lipase had relatively similar ability with R. miehei lipase, and the CPL lipase had the lowest ability. This study proved that rice bran and CPL lipases also had transesterification activity and showed the feasibility of the rice bran lipase to be a biocatalyst for n-3 PUFA-rich glyceride production. Increasing the substrate ratio, of n-3 PUFA concentrate and tuna or palm oil, could increase the EPA and DHA incorporation. The R. miehei, rice bran, and CPL lipases unabled to incorporate DHA into DHA-containing glyceride molecule, whereas C. antartica lipase had the capability in high ratio of n-3 PUFA concentrate to oil. Therefore, the lipases were easier to incorporate n-3 PUFA into palm oil than tuna oil, since the TAG molecules of palm oil was not as complex as tuna oil. It could be suggested that the lipases did not only have acyl chain and positional specificity, but also the whole glyceride structure specificity.Keywords : acidolysis, lipase, n-3 PUFA, palm oil, specificity, tuna oil.
PEMANFAATAN BAHAN TUMBUHAN SEBAGAI BIOKATALISATOR DALAM PRODUKSI MINYAK SAWIT KAYA ASAM LEMAK OMEGA-3 1) [Using of Plant Biocatalisator for Omega-3 PUFA -Rich Palm Oil Production] Jenny Elisabeth; D. Siahaan; D. R. H. Simajuntak
Jurnal Teknologi dan Industri Pangan Vol. 13 No. 2 (2002): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Incorporaton  of omega-3 polyunsaturated fatty acids (n-3 PUFA) into red palm oil was investigated by using acidolysis process. Rice bran and Carica papaya latex (CPL) were used as biocatalyst. Acidolysis between red palm  oil and n-3 PUFA concentrate in free  fatty acid form was conducted without solvent, with weight ratio of n-3 PUFA concentrate to palm oil being 1:1. The n-3 PUFA concentrate was prepared from tuna oil by crystallization method. The study has demonstrated  that rice bran lipase showed higher activity to incorporate n-3 PUFA into red palm oil than CPL. The extent of EPA (eicosapentaenoic acid, C20:5) and DHA (docosahexaeboic acid, C22:6) incorporation were 3.4% and 12.7% with the rice bran lipase, and 1.7% and 3.2%  with the CPL. Furthermore, rice bran from several varities of paddy (Mamberamo, IR-64, Merah Munte , and Cirata) produced equal  incorporation of EPA and DHA into red palm oil. Rice bran from germinated seed of paddy  did not increase the n-3 PUFA incorporation into red palm oil. It means that rice bran in its natural form could be used and act as immobilized lipase. It is easier to remove the rice bran from reaction mixture and reuse the bran for 14 times without decreasing its enzyme activity.  
Study of Crude Palm Oil (CPO) Handling and Storage Process in Palm Oil Mills in an Effort to Improve CPO Quality and Reduce the Risk of Contaminants Formation Anita Syafrianti; Zulkifli Lubis; Jenny Elisabeth
Journal of Food and Pharmaceutical Sciences Vol 9, No 2 (2021): J.Food.Pharm.Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.2091

Abstract

Crude Palm Oil (CPO) is palm oil obtained from the extraction or compression process of oil palm fruit flesh and has not been purified. Palm oil is usually used for food, cosmetic industry, chemical industry, and animal feed industry. Increasingly open insight and increasing consumer awareness of the importance of food safety and quality assurance supported by technological developments and laboratory analysis methods, new types of contaminants in food products are also found. Currently, the Indonesian government is facing challenges regarding the issue of 3-monochloro-1,2-propanediol ester (3-MCPD Ester) and glycidyl ester (GE) in palm oil which can hamper Indonesia's palm oil trade in the future. The practice in the field affects the quality of CPO and the retrieval of low-quality CPO is mixed with high-quality CPO. And the length of time the process of pumping CPO from reclaimed tanks to VCT affects the quality of CPO. So this study aims to modify the pumping time and storage time of CPO from Reclaimed tanks to VCT by mixing samples from VCT (fresh CPO) and samples from Reclaimed tanks (lower quality CPO).
Study of Crude Palm Oil (CPO) Handling and Storage Process in Palm Oil Mills in an Effort to Improve CPO Quality and Reduce the Risk of Contaminants Formation Anita Syafrianti; Zulkifli Lubis; Jenny Elisabeth
Journal of Food and Pharmaceutical Sciences Vol 9, No 2 (2021): J.Food.Pharm.Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.2091

Abstract

Crude Palm Oil (CPO) is palm oil obtained from the extraction or compression process of oil palm fruit flesh and has not been purified. Palm oil is usually used for food, cosmetic industry, chemical industry, and animal feed industry. Increasingly open insight and increasing consumer awareness of the importance of food safety and quality assurance supported by technological developments and laboratory analysis methods, new types of contaminants in food products are also found. Currently, the Indonesian government is facing challenges regarding the issue of 3-monochloro-1,2-propanediol ester (3-MCPD Ester) and glycidyl ester (GE) in palm oil which can hamper Indonesia's palm oil trade in the future. The practice in the field affects the quality of CPO and the retrieval of low-quality CPO is mixed with high-quality CPO. And the length of time the process of pumping CPO from reclaimed tanks to VCT affects the quality of CPO. So this study aims to modify the pumping time and storage time of CPO from Reclaimed tanks to VCT by mixing samples from VCT (fresh CPO) and samples from Reclaimed tanks (lower quality CPO).
Co-Authors Anita Syafrianti D. R. H. Simajuntak D. Siahaan J M Purba P M Tambunan T Yuliani Zulkifli Lubis