Garuda - Garba Rujukan Digital

Dhirgo Adji

Universitas Gadjah Mada

Author-ID : 2895394

Veterinary

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Articles

THE EFFECT OF HIGH LIPID DIET LIPOPOLYSACCHARIDE AND LAPARATOMY SURGERY ON THE LEVEL OF C-REACTIVE PROTEIN SERUM IN SPRAGUE DAWLEY RATS EKD Dhirgo Adji
Jurnal Sain Veteriner Vol 25, No 2 (2007): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

C-reactive protein (CRP) is a member of the family of proteins known as pentraxins, and originally defined as a substance, observed in the serum or plasma of patient with acute infections, that reacted with the Cpolysaccharide of the pneumococcus. Serum concentrations of CRP increase as part of the inflammatory response to infection or acute injury. The aim of the study was to see the differences between acute and chronicstimuli in the increasing of CRP concentration in serum of Sprague Dawley rat. Twenty male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted for 5 days and given basal diet,containing normal fat(5% of fat) and water ad libitum. Rats were then divided into 4 groups of 5 each. Group 1 was used as control animal. The animals in this group were fed basal diet for 59 days without any othertreatments. Group 2 was animals fed high fat diet containin g20Yo of fat, for 59 days without any other treatments. Group 3 was animals fed basal diet for 59 days and in the day of 60 then injected with LPS from Escherichia coli 0111:B4, 1 mg/kg BW intraperitoneally, and Group 4 was animals fed basal diet for 59 days and in the day of 60 animals then laparatomized. Twenty four hours after all treatments, blood sample were collected from orbitally vein, serum was separated and used to analyze CRP concentration. Statistical analyzes using onew ay analyzeso fvariances howed that there were significance differences among Group 1,2,3 and 4 (p<0.05). Mean of CRP concentrations respectively from the highest to the lowest were: Group 3, Group 4, Group 2 and Group 1. Fromthe result of the study it can be concluded that CRP concentration of acute stimulus was higher than chronic stimulus.Key words : C-reactivep rotein, lipopolysaccharides, high fat diet, laparatomy.

IMUNOHISTOCHEMTSTRY METHOD TO DETECT C-REACTIVE PROTEIN IN ATHEROMA PLAQUES OF SPRAGUE DAWLEY RATS FED HIGH LIPID RATION Dhirgo Adji
Jurnal Sain Veteriner Vol 25, No 1 (2007): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Since Inflammation is believed to have role in pathogenesis of cardiovasculare vents, measurement of markers of inflammation has been proposed as a method to improve the prediction of the risk of these events. C-reactive protein (CRP) a major acute phase protein, has been associated with the presence and severity of atherosclerosis, and has been found to predict acute vasculare vents in prospective studies. The aim of this study was to analyse the relationship between appearance of CRP in aorta and atheroma plaque formation in Sprague Dawley rats after 59 days induction with high fat diet. Ten male Sprague Dawley rats, 1.5 months of age were used as experimental animals. Rats were adapted in l0 single cages for 5 days and given basal diet containing normal fat and water ad libitum. After adaptation, rats were divided in to 2 groups (group 1 and group 2) of 5 each. Group I was fed basal diet containing normal fat(4.5% of fat) and group 2 was fed diet containing high fat (20% of fat). After 59 days, all rats were killed, the heart including aorta were taken out for histophatologic (HE) and immunohistochemistry analyses. The result of this study showed that all rats in Group 1 did not have atheroma plaque and CRP negative, but rats in G"cup 2, all have atheroma plaque (100%) but only 60% CRP positive. From those result it concluded that although all aortas have atheroma plaque, the CRP may not be detected in the plaque.Keywords: atheroma plaques, inflammation, C-reactive protein.

PERBANDINGAN EFEKTIVITAS STERILISASI ALKOHOL 70%, INFRAMERAH, OTOKLAF DAN OZON TERHADAP PERTUMBUHAN BAKTERI Bacillus subtilis Dhirgo Adji; . Zulianti; Henry Larashanty
Jurnal Sain Veteriner Vol 25, No 1 (2007): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Penelitian ini bertujuan untuk membandingkan efektivitas antara sterilisasi menggunakan alkohol 70%, otoklaf, inframerah dan ozon terhadap pertumbuhan bakteri berspora Bacillus subtilis. Lima belas buah jarum jahit yang masing-masing mengandung biak murni Bacillus subtilis dimasukkan ke dalam tabung steril. Tiga buah jarum digunakan sebagai kontrol positif tanpa perlakuan sterilisasi (Kelompok I), tiga buah jarumdisterilisasi menggunakan alkohol 70% selama 3 jam (Kelompok II), tiga buah jarum disterilisasi menggunakan inframerah selama 15 menit (kelompok III), tiga buah jarum disterilisasi menggunakan otoklaf selama 20 menit pada suhu 121◦C (Kelompok IV), dan tiga buah jarum disterilisasi menggunakan ozon selama 45 menit (Kelompok V ). Hasil dari penelitian ini menunjukkan bahwa sterilisasi dengan alkohol 70%, Bacillus subtilis masih tetap tumbuh. Sterilisasi dengan inframerah menunjukkan tidak ada pertumbuhan bakteri Bacillussubtilis. Sterilisasi dengan otoklaf satu sampel positif tumbuh sedangkan dua sampel yang lain negatif (bakteri tidak tumbuh), dan sterilisasi dengan ozon menunjukkan Bacillus subtilis tetap tumbuh. Dari penelitian ini disimpulkan bahwa sterilisasi menggunakan inframerah adalah yang paling efektif diantara metodas terilisasi yang lain.Kata kunci : sterilisasi, alkohol inframerah, otoklaf, ozon, Bacillus subtilis

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