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Hastari Wuryastuti
Fakultas Kedokteran Hewan Universitas Gadjah Mada
Veterinary

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Isolation, Identification and Characterization of the Surface Glycoprotein of Myxobolus koi Insariani Insariani; Khumaira Puspasari; Haririyah Haririyah; Emei Widiyastuti; Chusnul Chotimah; Hastari Wuryastuti
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8529.172 KB) | DOI: 10.22146/jsv.2491

Abstract

Myxobolus koi is considered to be one of the pest diseases of  fish quarantine class I, which has to be destroyed if it is found. Technique that is regularly used for diagnosis M. koi, such as histopathological examination, polymerase  chain reaction (PCR) and sequencing always done in dead fishes. The objective of this study was to isolate the protein of M. koi as the initial step for producing antibody that can be used as a material for immunology-based diagnostic method. In the present study. identification of surface protein of M. koi involved three different stages. The first stage was to isolate and identify M. koi from koi fish using wet mount, and histopathologic, PCR and sequencing techniques. In the second stage, purification and isolation of the surface protein of M. koi  was done by Percoll gradient ultracentrifugation technique. Purification using the technique produced 5 different layers on the surface of the tube . Myxobolus koi spores were found in the 2nd, 3rd and 4th layers. The third stage was to identify and characterize the surface protein of M. koi using SDS PAGE. The results of SDS PAGE analysis showed that spores purified from 3 different layers all have the same profiles. Molecular sizes of the surface protein of M. koi that could be isolated in this study were 12 Kd, 16 Kd, 25 Kd, 27 Kd, 40 Kd and 45 Kd. Among them, the molecular sizes of 12 Kd, 25 Kd and 27 Kd could be seen clearly.Keywords: Myxobolus koi, surface glycoprotein, isolation, Percoll gradient centrifugation, SDS PAGE
The Effect of Turmeric (Curcuma Domestica Val.) Extract on the Histopathological Lesions of Lung Induced by Cigarette Smokes in Wistar Rats laelatul Rahmad; Nida Ul Milah; Agapietta Kusumawardani; Novi Herliyani; Kurniati Sarwendah; Bambang Sutrisno; Hastari Wuryastuti; R Wasito
Jurnal Sain Veteriner Vol 33, No 1 (2015): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.089 KB) | DOI: 10.22146/jsv.8109

Abstract

This present study is aimed to observe and determine the effects of turmeric (Curcuma domestica Val.) extract administration towards the cytotoxicity effect of cigarette smokes in the lungs of Wistar rats. Twenty Wistar rats, male, 3 months old, were used and divided randomly into four groups, of five each. Group I is as a control (K I), Group II was given 9 cigarettes/day without turmeric extract administration (K II), Group III giventurmeric extract 1 ml 0.25%/day (K III) and Group IV was given 9 cigarettes/day and turmeric extract 0.25%/day (K IV) for 60 days. At the end of the present study, all rats were necropsied and organs, particularly the lungs were collected, processed histopatologically and stained routenely with hematoxilyn eosin. The results indicated that the lungs had macrophages infiltration in the septal alveoli and hyperplasia of bronchial associated lymphoidtissue (K II). Whereas, the lungs in the rats in Groups K I, K III and K IV were still within the normal limits. It is concluded, that turmeric extract may have a potential antipulmonary cytotoxity effect due to cigarette smoke.
Co-Authors Agapietta Kusumawardani Bambang Sutrisno Chusnul Chotimah Emei Widiyastuti Haririyah Haririyah Insariani Insariani Khumaira Puspasari Kurniati Sarwendah laelatul Rahmad Nida Ul Milah Novi Herliyani R Wasito