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Eryl Sri Rohayati
Bagian Parasitologi Fakultas Kedokteran Hewan UGM Yogyakarta
Veterinary

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THE EFFECT OF SEVERAL DESINFECTANTS ON THE SPORULATION OF THE ERIMERIA TENELLA OOCYST Eryl Sri Rohayati; Julianti Darjono; Wisnu Nurcahyo
Jurnal Sain Veteriner Vol 17, No 1 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8592

Abstract

A research has been conducted to evaluate the effect of certain desinfectants on the speculation of the Eimerla tenclla oocysts.In this research in vitro methode has been used. This research used oocysts Eimeria tenella which have not yet Sporulated. 6 kinds of disinfectants and water as control. These desinfectants were used in the research namely : Lysol, Creolin. Kalium Jodida (KJ). Formalin, Bromoquod and Ca (OH)2.The oocysts which have not yet sporulation were imerted in these desinfectants. The duration of liners ion at each treatment was succescively 10 minutes. 20 minutes and 30 minutes. The desinfectants concentration used is of three degree of strength, in the following proportion = 1:2:4.The data colected was the percentage of oocystys which have sporulated 24 hours, 48 hours and 72 hours, after treatment. The data was analyzed with the Split Plot method (Gill. 1978).The result of research showed that each desinfectant had a different effect on the percentage of the oocyst sporulation (P < 0,01) . Bromoquod was found to be the best desinfectant, and the others were in this following order = Creolin. KJ, Water, Lysol. CafQHJn and formalin. The Concen­tration was also found significantly effective (P < 0,6) in suppressing the sporulation of the oocysts. Creolin concen­tration of the 4tri strength was found to be the best desin­fectant and the others were in this following order : water. KJ. Bromoquod, Lysol, Ca(OH)£ and formalin. The duration of imersion had no significant influence
THE EFFECT OF STORAGE LENGTH ON THE PATHOGENICITY OF EIMERIA TENELLA OOCYSTS Eryl Sri Rohayati
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8598

Abstract

A research has been conducted to evaluate storage length effect on the pathogeniclty of E. tenella oocysts in broiler chickens.Two groups consist eight day old chickens, four chickens in group I and four chichkens in group II were used in this research. At the age of seventeen days, all the chickens in both groups were infected with 20,000 E. tenella oocysts. Group I was infected with Eimeria tenella oocyst that had been stored for 107 days at 4°C in the refrigerator and group II was infected with E. tenella oocysts that had been stored for two days at room temperature.The data collected were the caeca lesion score and the histopathology of the caeca. Of all chichkens in both groups that were killed on the fifth days after infection.The data of the caeca lesion score was analysed using the Rank test (Ferguson and Takane, 1989) while the data of caeca histopathology was analyzed descriptively.The result of the research on the caeca lesion score showed that there was a significant difference between group I and group II (P < 0,01). In group I the caeca lesion score was relative light (0) - (+1) if compared with group two (+4). The result of the research on the caeca histopathology showed that in group I the histopathological changes were relative light if compared with group II. In group II there was a great inflamation reaction and the coccidia stadia varied greatly.The conclusion of the research was when oocysts storage for 107 days will be influence the pathogenicity of the E. tenel1 a oocyst s.
Co-Authors Julianti Darjono Wisnu Nurcahyo