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Sumartono Sumartono
Bagian Parasitologi Fakultas Kedokteran Hewan UGM Yogyakarta
Veterinary

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THE EFFECT OF INCUBATION TIME ON EXCYSTATION OF EIMERIA TENELLA SPOROZOITES IN VITRO Joko Prastowo; Mufasirin Mufasirin; Sumartono Sumartono
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8596

Abstract

This purpose of the research is to know the effect of incubation time on excystation of Eimeria tenella sporo-zoites in vitro.Eimeria tenella oocystes treated in clorox were broken by glass beads. Sporocystes liberated from oocys were divid­ed in six groups (I - VI), each ghroup contained 1.68.106 sporocystes. All of the groups were treated by 0,75% taur-ocholat sodium and 0,25% trypsin. Those sporocystes were incubated at 41°C for 15, 30, 45, 60, 75 and 90 minutes respectively. The data collected was the percentage at the number of sporozoites found in each group.The result showed that the increase of the number of sporozoites was paralelly with the incubation time. The highest number of sporozoites excystation was found in the 90 minutes of incubation group.
Co-Authors Joko Prastowo Mufasirin