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Kajian Produksi Aflatoksin B1 Kasar dari Isolat Kapang Aspergillus Flavus Lokal pada Media Jagung dan Jagung+Kacang Tanah listya purnamasari; Ali Agus; Cuk Tri Noviandi
Buletin Peternakan Vol 40, No 2 (2016): BULETIN PETERNAKAN VOL. 40 (2) JUNI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i2.9354

Aflatoxin, which known as toxigenic compound, is a secondary metabolite produced by Aspergillus flavus and A. parasiticus. Aflatoxin is found in both food and feed stuffs, such as: corn and peanut. Aflatoxin standard is needed in every research of aflatoxin contamination. But it is rather difficult to get. It is imported, high costs, and take times. The aim of the research is to assess the potential local isolate of A. flavus to producing aflatoxin that can be used to be the alternative aflatoxin standard. Local mold of A. flavus FNCC 6122 and FNCC 6109 are got from PAU University of Gadjah Mada. Isolate was enriched on PDA medium for 5 days and move to corn medium of corn+peanut combine medium for 10-15 day at 25ºC. The variables was the content of AFB1 by ELISA test. The result is shown that isolat FNCC 6122 produced higher AFB1 than isolate FNCC 6109. The combine of corn and peanut medium stimulated FNCC 6122 to produce higher AFB1 and has potency to be the candidate of standard aflatoxin. In conclusion, the peanut addition on the medium would initiate increasing of the aflatoksin B1 level.

TEKNOLOGI PEMANFAATAN LIMBAH PADAT PENGOLAHAN KERIPIK SINGKONG MENJADI PAKAN PELET AYAM PEDAGING DI DESA BARATAN KABUPATEN JEMBER Listya Purnamasari; Nurul Pratiwi; Tri Agus Siswoyo
Journal of Livestock Science and Production Vol 2, No 1 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v2i1.687

Baratan Village, Patrang Sub-district, Jember District has a high potential production of cassava chips by Home Industry (IRT). Solid waste of cassava (cassava husk) from cassava processing into cassava chips has not been utilized properly. Cassava husk can be used as raw material for animal feed. The purpose of this program is the empowerment of the community of Baratan IRT in the processing of cassava husk into broiler feed and to increase the value of the waste and can improve the economic of the IRT in Baratan Village. Cassava husk has a deficiency because of the antinutrient content (Cyanide Acid) and dangerous when consumed. The methods used in this program are counseling and chipping of cassava with chipping tools, counseling and training of utilitation of cassava waste, counseling and training of broiler feed formulation, pelet making, mentoring, monitoring and evaluation. The result of this program is the IRT Baratan Village Jember Regency is able to process the solid waste from processing of cassava chips into broiler feed (pelet). Guidebooks can be used by IRT well so they can treat the waste into feed independently. IRT cassava chips as a partner of this program can implement and apply the technology that has been given and strive to always continuous in the manufacture of feed and pellet product development in an effort to improve the welfare and sustainability of the program.Keywords: cassava waste, pelet feed, Baratan Village

Physiological Response on Broiler Chicken’s Liver Supplemented Amino Acid Metionine-Cystine in Feed Contaminated with Aflatoxin B1 Listya Purnamasari; Ali Agus; Cuk Tri Noviandi
Journal of Livestock Science and Production Vol 3, No 1 (2019): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v3i1.1375

The content of AFB1 in feed with low levels and spend a long time, will cause primary damage or primary liver carcinoma. This study aims to reduce the toxicity of AFB1 with amino acid methionine-cystine supplementation in broiler chicken feed. This study used a 3 × 3 factorial design with methionine-cystine amino acid levels (M + C: 75, 100 and 125%) and AFB1 levels (0, 200, and 400 ppb). The variables collected were liver physiology, liver histopathology, SGPT levels, and SGOT levels. Observations of liver physiology showed that feed containing aflatoxin without methionine-cystine amino acid had a paler yellowish color (T4, T7 and T8). Pathological examination resulted that aflatoxicosis will attack the liver. Transition amino acid cystines in chicken feed contaminated with AFB1 did not occur in blood SGPT levels. Blood SGOT levels were highest at 21 days of age, namely T2 (M + C 100%) and T3 (M + C 125%) at AFB1 0 ppb which showed excess liver damage. The administration of methionine-cystine amino acids of 75, 100 and 125% in chicken feed contaminated with AFB1 0, 200 and 400 ppb consumed by broilers carries a risk of physiological and pathological damage to chicken liver.

RESPON FISIOLOGIS DAN PALATABILITAS DOMBA EKOR TIPIS TERHADAP LIMBAH TAUGE DAN KANGKUNG KERING SEBAGAI PAKAN PENGGANTI RUMPUT Listya Purnamasari; Sri Rahayu; Muhammad Baihaqi
Journal of Livestock Science and Production Vol 2, No 1 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v2i1.684

Physiological response are influenced by environmental conditions, such as climate change and the feed. Limitied of land crop and farm cause difficulties to find grass as fiber feed. This research aimed to study the effect of dried kale and bean sprouts waste for substitution the grass on the physiological response of male javanese thin thailed sheep (under one years old) and their palatability. Feed treatment were : P1 (50% concentrate+50% grass), P2 (50% concentrate+50% dried kale), and P3 (50% concentrate+50% bean sprouts waste) respectively. The parameters were palatability, rectal temperature, heartbeat and respiration frequency. Analyze method used completely randomized design with three treatments of feeding and 4 replications was used. Data processed by ANOVA and Tukey’s for further test. The experimental result showed that feed treatment were not significant (P>0.05) to rectal temperature, heartbeat and respiration frequency. The best palatability seen on P2 feed (50% consentrate + 50% mung bean sprout waste). The conclusion were bean sprout waste and dried kale can be used to substitute grass, they had a good palatability and they did not give bad effect to physiological of javanese thin tailed sheep.The conclusion of this study, bean sprout waste and dried kale has a good palatability and were not significant to physiological response of javanese thin tailed sheep so it can be used to substitute grass.Keywords: dried kale, physiological responses, javanese thin tailed sheep, bean sprout waste, palatability

Effect of transgenic and non-transgenic corn hybrids on the performance of quails and chicken: A review: Pengaruh hibrida jagung transgenik dan non-transgenik terhadap performa puyuh dan ayam: Review Himmatul Khasanah; Joaquin Rio V. Torres; Joseph F Dela Cruz; Listya Purnamasari; Desy Cahya Widianingru,
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 12 No. 3 (2022): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v12i3.308

Abstrak Ayam broiler, layer dan puyuh (Coturnix japonica) adalah jenis unggas yang semakin populer untuk dipelihara karena performa yang baik seperti produksi yang cepat, dan kemudahan perawatan, baik untuk penghasil daging dan telur di berbagai negara Asia dan di dunia. Unggas sering dipelihara dengan menggunakan jagung (Zea mays) sebagai sumber energi utama dalam ransum. Namun, dalam produksinya, jagung sering menghadapi masalah yang melibatkan hama arthropoda seperti Ngengat Penggerek Jagung Asia (Ostrinia furnacalis), dan oleh karena itu, teknologi rekayasa dalam bidang pertanian telah banyak mengembangkan banyak galur jagung transgenik yang telah ditanam dan dibiakkan agar tahan terhadap hama ini. Artikel ini bertujuan untuk mengetahui kinerja jagung transgenik sebagai pakan, keamanan dan kesetaraan gizi pada unggas baik sebagai penghasil daging maupun telur. Hasil review, menunjukkan bahwa di berbagai penelitian, tidak ada perbedaan yang signifikan antara parameter produksi pada ayam pedaging, petelur dan puyuh yang diberi pakan jagung non-transgenik konvensional dan transgenik. Kesamaan ini terlihat pada jenis unggas pedaging dan petelur. Penelitian untuk menganalisis efek jagung transgenik pada parameter komposisi kuning telur, dan komposisi otot ayam pedaging masih perlu dilakukan. Hasil kajian ini juga mengamati bahwa tidak ada gen dan protein transgenik yang tersisa setelah pemotongan unggas di dalam otot dan jaringan, yang menunjukkan bahwa masalah keamanan gen dan protein transgenik tidak ditransfer dari jagung ke produk unggas. Kata kunci: Broiler; GMO; Kualitas daging; Pakan; Produksi telur Abstract Broiler, Layer and Japanese Quail (Coturnix japonica) are species of poultry that have become increasingly popular to raise due to their performance such as fast production, and ease of care, either for meat and egg producers in many Asian countries and worldwide. As poultry, they are often raised using corn (Zea mays) as the primary energy source in the ration. However, corn often faces problems involving arthropod pests such as the Asian Corn Borer Moth (Ostrinia furnacalis), and as such, agricultural engineering technology has developed many strains of transgenic corn that have been grown and bred to be resistant to these pests. This article aimed to determine the transgenic corn performance as feed, safety and nutritional equivalence on poultry (quails and chicken) for meat and egg producer. The review determined that across various studies, there were no significant differences between production parameters in the broiler, layer and quails fed a conventional non-GMO diet and entirely transgenic corn. This similarity was seen in both meat and layer types. Though, research still needs to be done to assess transgenic corn's effects on parameters of yolk composition and breast muscle composition. The study also observed that no transgenic genes and proteins remained after the slaughter of the poultry in the muscle and tissues, indicating the safety concerns of transgenic genes and proteins not being transferred from the corn to poultry. Keywords: Broiler; Egg production; Feed; GMO; Meat quality

Evaluation of coffee bean husk fermented by a combination of Aspergillus niger, Trichoderma harzianum, and Saccharomyces cerevisiae as animal feed Himmatul Khasanah; Desy Cahya Widianingrum; Listya Purnamasari; Ali Wafa; Seong-Gu Hwang
Jurnal Ilmu-Ilmu Peternakan Vol. 32 No. 3 (2022): December 2022
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2022.032.03.13

Abundant coffee bean husk acquires an alternative source of fiber for livestock feed, but a high level of the crude fiber of it became an obstacle. Solid-state fermentation technology using lignocellulolytic fungi is known to be able to improve the nutritional quality of feedstuff that have high fiber content. Its mechanism is through the degradation of the lignocellulose fraction and enhance protein content. This study aimed to determine the nutritional quality of fermented coffee bean husk with a combination of fungi and yeast. The fermentation method used a solid-state fermentation consisting of 7 different inoculums, namely: P0: Unfermented coffee bean husk, P1: Aspergillus niger, P2: Saccharomyces cerevisiae, P3: Trichoderma harzianum, P4: Aspergillus niger + S. Cereviciase, P5: Aspergillus niger + Trichoderma harzianum, P6: Saccharomyces cerevisiae + Trichoderma harzianum and P7: Aspergillus niger + Saccharomyces. Cereviciase + Trichoderma harzianum. The nutritional quality of the fermented coffee bean husk was determined by proximate analysis, lignocellulolytic fraction, and digestibility. The data obtained were analyzed by ANOVA and followed by Tukey's post hoc test. The crude fiber content of fermented coffee bean husk (P1-P7) was lower than unfermented (P0). There was no significant difference among treatments in crude fat and protein. Treatment P3 has the highest total digestibility nutrient (70) and the lower crude fiber (15.03). A combination of Aspergillus niger and Saccharomyces cerevisiae reduce lignin content by about (4,16%). In conclusion, the fermented coffee bean husk can be utilized as animal feedstuff with higher nutritional quality than unfermented.

Physiological Response on Broiler Chicken’s Liver Supplemented Amino Acid Metionine-Cystine in Feed Contaminated with Aflatoxin B1 Listya Purnamasari; Ali Agus; Cuk Tri Noviandi
Journal of Livestock Science and Production Vol 3, No 1 (2019): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v3i1.1375

The content of AFB1 in feed with low levels and spend a long time, will cause primary damage or primary liver carcinoma. This study aims to reduce the toxicity of AFB1 with amino acid methionine-cystine supplementation in broiler chicken feed. This study used a 3 × 3 factorial design with methionine-cystine amino acid levels (M + C: 75, 100 and 125%) and AFB1 levels (0, 200, and 400 ppb). The variables collected were liver physiology, liver histopathology, SGPT levels, and SGOT levels. Observations of liver physiology showed that feed containing aflatoxin without methionine-cystine amino acid had a paler yellowish color (T4, T7 and T8). Pathological examination resulted that aflatoxicosis will attack the liver. Transition amino acid cystines in chicken feed contaminated with AFB1 did not occur in blood SGPT levels. Blood SGOT levels were highest at 21 days of age, namely T2 (M + C 100%) and T3 (M + C 125%) at AFB1 0 ppb which showed excess liver damage. The administration of methionine-cystine amino acids of 75, 100 and 125% in chicken feed contaminated with AFB1 0, 200 and 400 ppb consumed by broilers carries a risk of physiological and pathological damage to chicken liver.

Pendampingan pengolahan pakan melalui teknologi Solid State Fermentation limbah kulit kopi di kelompok ternak sapi di Kabupaten Bondowoso, Jawa Timur Himmatul Khasanah; Desy Cahya Widianingrum; Nur Widodo; Roni Yulianto; Dwi Erwin Kusbianto; Listya Purnamasari; Eistifani Fajrin; Purnaning Dhian Isnaeni; Putrika Suryandari
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 9, No 3 (2025): May
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v9i3.30609

Abstrak Permasalahan pakan menjadi salah satu kendala yang dialami oleh masyarakat peternak sapi potong di kabupaten Bondowoso, Jawa Timur. Sistem pengoleksian pakan cut and carry sulit dilakukan pada musim kemarau. Sebagian besar lahan pertanian di kabupaten Bondowoso merupakan lahan Perkebunan kopi. Limbah kulit kopi yang dihasilkan dari industry kopi di kabupaten Bondowoso masih kurang optimal. Kulit kopi memiliki kandungan nutrient yang masih potensial untuk dapat digunakan sebagai pakan ternak. Keberadaan antinutrisi dan rendahnya kecernaan menjadi faktor pembatas dalam pemanfaatannya sebagai pakan ternak. Pengabdian ini bertujuan untuk meningkatkan keterampilan peternak dalam menyediakan pakan ternak sapi potong yang bernilai gizi tinggi asal limbah kulit kopi melalui teknologi solid state fermentation. Kegiatan diawali dengan sosialisasi mengenai pakan komplit untuk ternak sapi potong, pelatihan pengolahan pakan asal kulit kopi, dan monitoring pemberian pakan pada ternak. Hasik kegiatan pengabdian menunjukan bahwa Masyarakat peternak sangat antusias mengikuti kegiatan baik sosialisasi, pelatihan maupun monitoring. Ternak sapi potong yang diberikan pakan komplit asal limbah kulit kopi melalui teknologi SSF selama 4 bulan juga memiliki pertambahan bobot badan yang lebih tinggi yaitu 144 kg dibandingkan ternak sapi yang hanya diberi limbah kulit kopi tanpa diolah yaitu 120 kg. Kata kunci: Bondowoso; sapi; pakan; fermentasi; kulit kopi Abstract The issue of feed availability is one of the major challenges faced by the beef cattle farming community in Bondowoso District, East Java. The cut-and-carry feed collection system becomes particularly difficult during the dry season. Most of the agricultural land in Bondowoso District is used for coffee plantations. However, the utilization of coffee husk waste produced by the local coffee industry remains suboptimal. Coffee husks contain nutrients that have the potential to be used as animal feed. Nevertheless, the presence of antinutritional factors and low digestibility limit their effectiveness as feed. This community service initiative aims to enhance farmers' skills in providing nutritionally rich beef cattle feed by utilizing coffee husk waste through solid-state fermentation technology. The program began with a socialization session on complete feed for beef cattle, followed by training on how to process feed from coffee husks, and concluded with monitoring the implementation of feed usage for livestock. The results of the service activities show that the farming community was highly enthusiastic about participating in all aspects of the program, including socialization, training, and monitoring. Beef cattle that were fed complete feed made from coffee husk waste processed using solid-state fermentation (SSF) technology for four months showed a higher body weight gain of 144 kg, compared to those fed unprocessed coffee husk waste, which gained only 120 kg. Keywords: Bondowoso; cattle; feed; fermentation; coffee husk.

RESPON FISIOLOGIS DAN PALATABILITAS DOMBA EKOR TIPIS TERHADAP LIMBAH TAUGE DAN KANGKUNG KERING SEBAGAI PAKAN PENGGANTI RUMPUT Listya Purnamasari; Sri Rahayu; Muhammad Baihaqi
Journal of Livestock Science and Production Vol 2, No 1 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v2i1.684

Physiological response are influenced by environmental conditions, such as climate change and the feed. Limitied of land crop and farm cause difficulties to find grass as fiber feed. This research aimed to study the effect of dried kale and bean sprouts waste for substitution the grass on the physiological response of male javanese thin thailed sheep (under one years old) and their palatability. Feed treatment were : P1 (50% concentrate+50% grass), P2 (50% concentrate+50% dried kale), and P3 (50% concentrate+50% bean sprouts waste) respectively. The parameters were palatability, rectal temperature, heartbeat and respiration frequency. Analyze method used completely randomized design with three treatments of feeding and 4 replications was used. Data processed by ANOVA and Tukey’s for further test. The experimental result showed that feed treatment were not significant (P0.05) to rectal temperature, heartbeat and respiration frequency. The best palatability seen on P2 feed (50% consentrate + 50% mung bean sprout waste). The conclusion were bean sprout waste and dried kale can be used to substitute grass, they had a good palatability and they did not give bad effect to physiological of javanese thin tailed sheep.The conclusion of this study, bean sprout waste and dried kale has a good palatability and were not significant to physiological response of javanese thin tailed sheep so it can be used to substitute grass.Keywords: dried kale, physiological responses, javanese thin tailed sheep, bean sprout waste, palatability

TEKNOLOGI PEMANFAATAN LIMBAH PADAT PENGOLAHAN KERIPIK SINGKONG MENJADI PAKAN PELET AYAM PEDAGING DI DESA BARATAN KABUPATEN JEMBER Listya Purnamasari; Nurul Pratiwi; Tri Agus Siswoyo
Journal of Livestock Science and Production Vol 2, No 1 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jalspro.v2i1.687

Baratan Village, Patrang Sub-district, Jember District has a high potential production of cassava chips by Home Industry (IRT). Solid waste of cassava (cassava husk) from cassava processing into cassava chips has not been utilized properly. Cassava husk can be used as raw material for animal feed. The purpose of this program is the empowerment of the community of Baratan IRT in the processing of cassava husk into broiler feed and to increase the value of the waste and can improve the economic of the IRT in Baratan Village. Cassava husk has a deficiency because of the antinutrient content (Cyanide Acid) and dangerous when consumed. The methods used in this program are counseling and chipping of cassava with chipping tools, counseling and training of utilitation of cassava waste, counseling and training of broiler feed formulation, pelet making, mentoring, monitoring and evaluation. The result of this program is the IRT Baratan Village Jember Regency is able to process the solid waste from processing of cassava chips into broiler feed (pelet). Guidebooks can be used by IRT well so they can treat the waste into feed independently. IRT cassava chips as a partner of this program can implement and apply the technology that has been given and strive to always continuous in the manufacture of feed and pellet product development in an effort to improve the welfare and sustainability of the program.Keywords: cassava waste, pelet feed, Baratan Village

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