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All Journal Buletin Peternakan
Jamhari Jamhari
Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
Veterinary Other

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The Potential of Hydrolysate from Rabbit Meat Protein as an Angiotensin Converting Enzyme Inhibitor Edy Permadi; Jamhari Jamhari; Edi Suryanto; Zaenal Bachruddin; Yuny Erwanto
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.31495

Abstract

This research aimed to investigate the rabbit meat hydrolysate potential as an angiotensin-converting enzyme (ACE) inhibitor. Indonesian local rabbit meats were used in this study. The research was conducted in Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, from August 2016 to February 2017. The local rabbit meats were hydrolyzed by pepsin, trypsin, and pancreatic. The obtained hydrolysates were then analyzed to identify the water-soluble protein content. The molecular weight of the hydrolysates were also confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The ACE inhibitory properties of the hydrolysates were analyzed in vitro. The results showed that pepsin, trypsin, and pancreatic hydrolysis showed a significant effect on the water-soluble protein content of rabbit meat (p<0.05). The water-soluble protein of rabbit meat hydrolysed by pepsin, trypsin, and pancreatic were 9.41, 7.66, and 9.75 mg/mL respectively. The molecular weight of the rabbit meat hydrolysate were increased from 10 to 43 kDa; 17 to 43 kDa; and 10 to 43 kDa, after hydrolysed by by pepsin, trypsin, and pancreatic respectively. Furthermore, the ACE inhibitory properties ) of the hydrolysed rabbit meat by pepsin, trypsin, and pancreatic were 439, 170, and 380 μg/mL, respectively. The rabbit meat hydrolysate showed a potential to be ACE inhibitor after hydrolyzed with pepsin, trypsin and pancreatic. Moreover, it also showed a promising potential to be used as bioactive components in different pharmaceutical applications. The highest ACE inhibitory capability was showed on trypsin hydrolysis with the total of 65.45% and 170 μg/mL ACE inhibition
Physicochemical Properties and Antioxidant Activity of Chicken Sausage with Addition of Roselle Extract (Hibiscus sabdariffa) and Different Filler Ratio Jamhari Jamhari; Aryanti Candra Dewi; Setiyono Setiyono
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i1.33800

Abstract

 This study was done to determine the effect of Roselle extract level (Hibiscus sabdariffa), and the wheat flour and mocaf flour ratio, as well as the interaction between the Roselle extract level and the wheat flour and mocaf flour ratio on the chemical, physical, and antioxidant activity of chicken sausage. The ingredients used were chicken meat, wheat flour, mocaf flour, Roselle extract, skim milk powder, and spices. Roselle extract levels were 0, 4, and 8% (v/w of total dough) and ratio of wheat flour : mocaf flours were 100:0, 50:50 and 0:100. Parameters observed included chemical composition (moisture, protein, and fat contents), physical properties (pH value and tenderness), and antioxidant activity of chicken sausage. Data were analyzed by analysis of variance of 3x3 factorial and continued by Duncan's new multiple range test test. The results of statistical analysis showed that Roselle extract level gave a significant effect (P<0.05) on moisture content, pH value, and antioxidant activity of chicken sausage. Ratio of wheat flour : mocaf flour gave a significant effect (P<0.05) on protein content,  tenderness and antioxidant activity of chicken sausage. There was no interaction between Roselle extract level and the wheat flour : mocaf flour ratio on the physicochemical properties and antioxidant activity of chicken sausage. The conclusion of this study is the addition of Roselle extract upto 8% will increase moisture content and antioxidant activity, but it decreases the pH value and tenderness of chicken sausage. Subtitution of wheat flour with mocaf flour will reduce protein content, tenderness and antioxidant activity of chicken sausage.
Co-Authors Aryanti Candra Dewi Edy Permadi Setiyono Setiyono Suryanto, Edi Yuny Erwanto Zaenal Bachruddin