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All Journal Buletin Peternakan Pastura; Journal Of Tropical Forage Science
Nilo Suseno
Fakultas Peternakan Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Veterinary Other

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Callus Induction and Plant Regeneration of Brachiaria Grass from Immature Inflorescence Explants Nilo Suseno; Nofi Isnaini; Bambang Suwignyo; Bambang Suhartanto; Nafiatul Umami
Buletin Peternakan Vol 45, No 2 (2021): BULETIN PETERNAKAN VOL. 45 (2) MAY 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i2.43461

Abstract

This research was aimed to optimize concentration of phytohormone in callus induction and in vitro regeneration of three species of Brachiaria grass plant, namely Brachiaria brizantha, B. decumbens, and B. ruziziensis. Immature inflorescences were used as explant material. To induce callus, explants were inoculated into Murashige and Skoog (MS) basal medium supplemented with phytohormone combination of 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba and kinetin. Observation of callus induction included percentage of callus formation and callus morphology. The embryogenic calli were then transferred into regeneration media, i.e. MS basal medium supplemented with kinetin and 6-benzylaminopurin (BAP). The result showed that 4 mg/L 2,4-D + 0.2 mg/L kinetin showed highest callus induction in B. brizantha and B. decumbens, namely  76%  and 88% respectively. Whereas in B. ruziziensis, 3 mg/L 2,4-D + 0.2 mg/L kinetin showed highest callus induction, namely 86%. MS medium supplemented with 4 mg/L kinetin showed highest regeneration in all three grass species, namely 92.5% in both B. brizantha and B. ruziziensis, and 88.75% in B. decumbens.
Effect of Organic and Inorganic Fertilizers on Yield and Quality of Synedrella nodiflora (Tropical Weed) Bambang Suwignyo; Galih Pawening; Muhammad Humaidi Haris; Nafiatul Umami; Nilo Suseno; Bambang Suhartanto
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.56115

Abstract

This study aimed to determine the effect of organic and inorganic fertilizers on the cultivation of tropical weed Synedrella nodiflora as forage. The study was conducted from May to July 2018. The treatments of the research were control (C), organic fertilizer (D) and organic fertilizer + urea (DU), with 3 replications that were arranged on experimental design with a completely randomized design, in a unidirectional pattern and continued with least significant different (LSD). Organic fertilizer dosage in this study was 5 tons/ha, while urea fertilizer was 350 kg/ha, with plant spacing was 45x60 cm. The observed parameters were plant height, forage production and chemical composition (5 weeks after planting). Plant height of C, D and DU were 41.59, 47,42, and 50.59 cm respectively. Forage production of dry matter after 5 weeks planting at C, D and DU were 1.69, 1.70 and 2.91ton/ha, with in vitro digestibility values ranging from 51.68 to 57.70% (IVDMD) and 51.71 to 61.98% (OMD) respectively. The chemical composition of native S. Nodiflora were 12.32% of dry matter (DM), 62.45% TDN count for cattle and 67.42% TDN count for sheep. Based on DM, The organic matter was 84.46%, crude protein 20,11%, crude fiber 13.26%, extract ether 7.77%, and nitrogen free extract 37.08%. The combination of organic fertilizer and urea increased the height and fresh and dry matter production S. nodiflora.
MORFOLOGI DAN PRODUKSI BIOMASSA Chloris gayana cv. Callide, DAN Megathyrsus maximus cv. Gatton PADA TAHUN PERTAMA PENANAMAN DI YOGYAKARTA Nafiatul - Umami; Nilo - Suseno
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 11 No 1 (2021): Pastura Vol. 11 No. 1 Tahun 2021
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/Pastura.2021.v11.i01.p02

Abstract

Penelitian ini bertujuan mengetahui karakter morfologi dan produksi biomasa Chloris gayana cv. Callidedan Megathyrsus maximus cv. Gatton yang diintroduksi di Yogyakarta pada tahun pertama penanaman.Tanaman tersebut ditanam dengan menggunakan biji dalam plot ukuran 2x2 m dengan cara menebar bijidan masing-masing spesies ditanam dengan 5 replikasi. Data yang diamati meliputi karakter morfologi,produksi biomasa, kadar bahan kering dan bahan organik. Data yang diperoleh dianalisis secara deskripsidan dengan metode t-test. Rumput yang diintroduksi merupakan tanaman perennial. Pertumbuhan tanamanmenunjukkan pertumbuhan yang baik. Morfologi tanaman normal sesuai karakter kedua tanaman ini. Chloris gayana cv. Callide memiliki produksi hijauan kering 25,21 ton/ha/ tahun pada tahun pertama (kadar BK 19,70% dan kadar BO 88,20%). Megathyrsus maximus cv. Gatton memiliki produksi hijauan kering 32,44 ton/ha/tahun pada regrowth kedua (kadar BK 19,85% dan kadar BO 88,18%). Berdasarkan penelitian dapat disimpulkan bahwa Megathyrsus maximus cv. Gatton memiliki produksi biomassa lebih tinggi dari Chloris gayana cv. Callide. Kata kunci: Chloris gayana, Megathyrsus maximus, morfologi, perennial, produksi biomassa, regrowth
Co-Authors Bambang Suhartanto Bambang Suwignyo Galih Pawening Muhammad Humaidi Haris Nafiatul Umami Nofi Isnaini