Muhlisin Muhlisin
Faculty Of Animal Sciences, Universitas Gadjah Mada

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Gamma Radiation Effect on Growth, Production and Lignin Content of Sorghum sudanense at Different Harvest Ages Maudi Nayanda Delastra; Andriyani Astuti; Bambang Suwignyo; Muhlisin Muhlisin; Nafiatul Umami
Buletin Peternakan Vol 45, No 3 (2021): BULETIN PETERNAKAN VOL. 45 (3) AUGUST 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i3.62627

Abstract

 The purpose of this study was to determine the effect of gamma-ray irradiation on Sorghum sudanense in the first offspring (F1) on plant growth, production, and lignin content at different harvest ages. This study used a split-plot design in which varieties of Sorghum sudanense with gamma irradiation and Sorghum sudanense without gamma irradiation are the main plot. Meanwhile, the harvest age is the subplot. The planting area was 1.5x1.5 m, each with 3 replications. The materials used were Sorghum sudanense without gamma-ray irradiation and the first generation seeds (F1) of Sorghum sudanense with gamma irradiation. The method used was irradiating Sorghum sudanense seeds with gamma-ray with a dose of 300 Gy, planting, maintenance, and harvesting. The harvest ages were 50, 70, and 90 days. The data observed were plant growth, namely plant height and length, dry matter (DM) and organic matter (OM) production, and lignin content. Sorghum sudanense with gamma irradiation had higher plant height, plant length, also DM, and OM production (P<0.05) than Sorghum sudanense without gamma irradiation. The lignin content of Sorghum sudanense with gamma irradiation was lower (P<0.05) than Sorghum sudanense without gamma irradiation. Longer harvest age increased (P<0.05) plant height, plant length, production, and lignin content. In conclusion, there were characteristics differences between Sorghum sudanense with gamma irradiation and without gamma irradiation (parents). The longer harvest led to higher plant height, length, production, and lignin content. There was an interaction (P<0.05) between varieties and harvest ages. Gamma irradiated Sorghum sudanense had a peak production at the age of 70 days, with a lignin content of 3.63%.
The Effect of Protected Lemuru Fish Oil Supplementation on In Vivo Nutrient Digestibility and Sheep Blood Profile Ratri Ratna Dewi; Kustantinah Kustantinah; Muhlisin Muhlisin
Buletin Peternakan Vol 46, No 1 (2022): BULETIN PETERNAKAN VOL. 46 (1) FEBRUARY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i1.70339

Abstract

This research was intended to observe the effect of protected lemuru fish (Sardinella longiceps) oil for ruminants, especially for sheep. This study aimed to evaluate the digested nutrients and blood profile of sheep. This study was conducted in September-October 2020 in Dumbira Farm, Kalasan, Yogyakarta, using 12 sheep divided into 3 treatments with 4 replications each. Treatment P0 was Total Mixed Ration (TMR) without protected Lemuru fish oil (control), treatment P1 was TMR with 5% protected Lemuru fish oil, and treatment P2 was TMR with 10% protected Lemuru fish oil. The data were statistically analyzed using one way analysis of variance and continued with Duncan new Multiple Range Test for significant results. The results of this study indicated that the addition of 10% protected Lemuru fish oil in TMR feed had a significant effect (P<0,05) on the increased value of in vivo digestion of crude fiber and crude fat, but did not affect the digestibility of dry matter, organic matter, and protein. The addition of protected Lemuru fish oil did not cause hematological disorders showed by the blood profiles were in the normal range. In conclusion, protected lemuru fish oil supplementation had a favorable influence on the production performance without affecting blood profile of sheep.
Maja Fruit Extracts Inhibit Escherichia coli, Reduce Fly Larvae Population, and Ammonia Emission of Chicken Excreta B. L. Syaefullah; Y. Erwanto; Wihandoyo; Muhlisin; R. A. Prasetyo; N. Kurniawati; N. A. Fitriyanto
Tropical Animal Science Journal Vol. 43 No. 4 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2020.43.4.369

Abstract

The characteristic of chicken excreta has a very potential as a breeding media for flies and is known for causing odorous pollutants (NH3 emission) from undigested protein and the activities of urease microorganisms. This study is focused on extracting Maja fruit, to quantify marmelosin from different fruit conditions using HPLC, and to determine the biological activity for handling the chicken excreta problems. In this study, the Kirby-Bauer Test was used to observe the antibacterial activity of marmelosin, the NH3 trapping method was used to determine ammonia emission, and the larvae population was determined by the Fly-Grill method. Marmelosin contents in MFE from immature, mature, and fermented fruit condition were 108.65 μg/g; 65.83 μg/g, and 23.02 μg/g, respectively. The increasing level of marmelosin addition to 50, 100, 150, and 200 μg/mL caused the higher diameter of inhibition zone against E. coli (p<0.05), which were 2.50, 2.90, 5.06, and 7.27 mm, respectively. The increasing level of MFE addition at 5, 7.5, and 10% (v/v) showed a higher inhibition effect on the NH3 emission from the excreta. The addition of MFE up to 10% (v/v) had no significant effect on the total larvae population of flies that existed in the excreta. It can be concluded that the highest marmelosin content was confirmed in the immature fruit condition. The highest antibacterial activity of marmelosin from MFE was shown at the concentration of 200 µg/mL. The application of 10% (v/v) MFE to the excreta gave the highest inhibition of NH3 emission and minimized the average larvae population of flies.