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Grace Tj. Sulungbudi
Pusat Sains dan Teknologi Bahan Maju-BATAN, Kawasan Puspiptek Serpong, Tangerang Selatan
Chemical Engineering, Chemistry & Bioengineering Education Social Sciences

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Pengaruh Rasio Doxorubisin:Apoferritin terhadap Kapasitas dan Efisiensi Enkapsulasi Doksorubisin dalam Apoferritin Novita Wiwoho; Maria Christina Prihatiningsih; Mujamilah Mujamilah; Grace Tj. Sulungbudi
Jurnal Rekayasa Proses Vol 11, No 1 (2017)
Publisher : Departemen Teknik Kimia Fakultas Teknik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.842 KB) | DOI: 10.22146/jrekpros.24868

Abstract

Doxorubicin is a chemotherapy drug which is very toxic and causes many side effects. To reduce side effects, doxorubicin can be encapsulated by apoferritin into apoferritin doxorubicin (Apo-Dox) system. In this research, various mass of doxorubicin i.e. 0.17 mg (S1), 0.26 mg (S2), 0.35 mg (S3), and 0.52 mg (S4) were encapsulated with 21.50 mg apoferritin. Encapsulation process was carried out by lowering pH medium for apoferritin dis-assembly, doxorubicin addition and dialysis for gradual and controlled pH-increase of medium to support re-assembly of apoferritin and doxorubicin encapsulation. End-result samples were then centrifuged and washed to separate the unreacted doxorubicin and apoferritin’s subunits. Doxorubicin encapsulation efficiency was determined using microplate reader spectrophotometry. The highest encapsulation capasity was 3.87 g dox/mg apo for S4 samples. Increasing the weight of doxorubicin gives more significant effect on increasing the reactive weight of apoferritin, which reached 93.73% (S4 sample). Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis confirm that apoferritin is in the Apo-Dox sample. ABSTRAKDoksorubisin merupakan obat kemoterapi yang sangat toksik dan menimbulkan berbagai efek samping. Untuk mengurangi efek samping tersebut, doksorubisin dapat dibungkus dengan protein apoferritin menjadi Apoferritin-Doksorubisin (Apo-Dox). Dalam penelitian ini, doksorubisin dengan berat 0,174 mg (S1), 0,261 mg (S2), 0,349 mg (S3), dan 0,523 mg (S4) dienkapsulasi apoferritin dengan berat 21,487 mg. Proses enkapsulasi dilakukan dengan mengubah pH larutan melalui penambahan glisin asetat, tris base, dan dialisa dalam tris asetat. Larutan di centrifuge dan di cuci untuk menghilangkan doksorubisin dan sub-unit apoferritin yang tidak bereaksi. Konsentrasi doksorubisin yang terenkapsulasi dalam apoferritin (μg dox/mg apo) ditentukan menggunakan microplate reader spectrophotometry. Konsentrasi cenderung mencapai optimum mulai sampel S2 dan hanya sedikit meningkat untuk sampel S3 dan S4 dengan nilai maksimum pada S4 sebesar 3,87 μg dox/mg apo. Peningkatan jumlah doksorubisin akan meningkatkan jumlah apoferritin yang bereaksi, dan diperoleh nilai efisiensi reaksi apoferritin tertinggi 93,73% untuk sampel S4. SDS-PAGE membuktikan bahwa apoferritin berada pada sampel Apo-Dox.
Pengaruh Rasio Doxorubisin:Apoferritin terhadap Kapasitas dan Efisiensi Enkapsulasi Doksorubisin dalam Apoferritin Novita Wiwoho; Maria Christina Prihatiningsih; Mujamilah Mujamilah; Grace Tj. Sulungbudi
Jurnal Rekayasa Proses Vol 11, No 1 (2017)
Publisher : Departemen Teknik Kimia Fakultas Teknik Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jrekpros.24868

Abstract

Doxorubicin is a chemotherapy drug which is very toxic and causes many side effects. To reduce side effects, doxorubicin can be encapsulated by apoferritin into apoferritin doxorubicin (Apo-Dox) system. In this research, various mass of doxorubicin i.e. 0.17 mg (S1), 0.26 mg (S2), 0.35 mg (S3), and 0.52 mg (S4) were encapsulated with 21.50 mg apoferritin. Encapsulation process was carried out by lowering pH medium for apoferritin dis-assembly, doxorubicin addition and dialysis for gradual and controlled pH-increase of medium to support re-assembly of apoferritin and doxorubicin encapsulation. End-result samples were then centrifuged and washed to separate the unreacted doxorubicin and apoferritin’s subunits. Doxorubicin encapsulation efficiency was determined using microplate reader spectrophotometry. The highest encapsulation capasity was 3.87 g dox/mg apo for S4 samples. Increasing the weight of doxorubicin gives more significant effect on increasing the reactive weight of apoferritin, which reached 93.73% (S4 sample). Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis confirm that apoferritin is in the Apo-Dox sample. ABSTRAKDoksorubisin merupakan obat kemoterapi yang sangat toksik dan menimbulkan berbagai efek samping. Untuk mengurangi efek samping tersebut, doksorubisin dapat dibungkus dengan protein apoferritin menjadi Apoferritin-Doksorubisin (Apo-Dox). Dalam penelitian ini, doksorubisin dengan berat 0,174 mg (S1), 0,261 mg (S2), 0,349 mg (S3), dan 0,523 mg (S4) dienkapsulasi apoferritin dengan berat 21,487 mg. Proses enkapsulasi dilakukan dengan mengubah pH larutan melalui penambahan glisin asetat, tris base, dan dialisa dalam tris asetat. Larutan di centrifuge dan di cuci untuk menghilangkan doksorubisin dan sub-unit apoferritin yang tidak bereaksi. Konsentrasi doksorubisin yang terenkapsulasi dalam apoferritin (μg dox/mg apo) ditentukan menggunakan microplate reader spectrophotometry. Konsentrasi cenderung mencapai optimum mulai sampel S2 dan hanya sedikit meningkat untuk sampel S3 dan S4 dengan nilai maksimum pada S4 sebesar 3,87 μg dox/mg apo. Peningkatan jumlah doksorubisin akan meningkatkan jumlah apoferritin yang bereaksi, dan diperoleh nilai efisiensi reaksi apoferritin tertinggi 93,73% untuk sampel S4. SDS-PAGE membuktikan bahwa apoferritin berada pada sampel Apo-Dox.
Pengaruh rasio doxorubisin: Apoferritin terhadap kapasitas dan efisiensi enkapsulasi doksorubisin dalam apoferritin Novita Wiwoho; Maria Christina P.; Mujamilah; Grace Tj. Sulungbudi
Jurnal Rekayasa Proses Vol 11 No 1 (2017): Volume 11, Number 1, 2017
Publisher : Jurnal Rekayasa Proses

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jrekpros.24868

Abstract

Doxorubicin is a chemotherapy drug which is very toxic and causes many side effects. To reduce side effects, doxorubicin can be encapsulated by apoferritin into apoferritin doxorubicin (Apo-Dox) system. In this research, various mass of doxorubicin i.e. 0.17 mg (S1), 0.26 mg (S2), 0.35 mg (S3), and 0.52 mg (S4) were encapsulated with 21.50 mg apoferritin. Encapsulation process was carried out by lowering pH medium for apoferritin dis-assembly, doxorubicin addition and dialysis for gradual and controlled pH-increase of medium to support re-assembly of apoferritin and doxorubicin encapsulation. End-result samples were then centrifuged and washed to separate the unreacted doxorubicin and apoferritin’s subunits. Doxorubicin encapsulation efficiency was determined using microplate reader spectrophotometry. The highest encapsulation capasity was 3.87 g dox/mg apo for S4 samples. Increasing the weight of doxorubicin gives more significant effect on increasing the reactive weight of apoferritin, which reached 93.73% (S4 sample). Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis confirm that apoferritin is in the Apo-Dox sample.
Co-Authors Maria Christina P. Maria Christina Prihatiningsih Mujamilah Mujamilah Mujamilah Novita Wiwoho Novita Wiwoho