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Irfanul chakim
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VARIASI GENETIK ANOPHELES SUNDAICUS BERDASARKAN SEGMEN ITS2 DNA RIBOSOM DAN GEN COI DNA MITOKONDRIA Irfanul chakim
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Entomological study with laboratory analysis done on Anopheles sundaicus mosquitoes as a malaria vector. The aim is to determine the existence of genetic variation in the Anopheles sundaicus species. PCR was used for the molecular identification of An. Sundaicus using COI and ITS2 genes. COI and ITS2 sequences in the study group compared with An. Epiroticus from GenBank. The comparation were done using a multiple sequence alignment program of Bioedit 7.1.9. The phylogenetic tree were constructed using neighbor-joining and bootstrap- tested by MEGA6. ITS2 sequence alignment results showed a single nucleotide substitution that can be used as markers for species determination. Phylogenetic tree analysis based on COI gene showed that An. Sundaicus in this study with isolates from GenBank, separate into two different groups or are polyphyletic. This study proved that An. Sundaicus from field collection was An. Epiroticus species by molecular identification. This study can be used to help public health policy related to vector control.Keywords: PCR, COI, ITS2, An. Sundaicus, An. Epiroticus
Molecular Surveillance of Pyrethroid Resistance of Dengue Vector [Aedesaegypti] and its Implication To Public Health Sayono -; Ulfa Nurullita; Irfanul Chakim
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract.Resistance of Aedesaegypti mosquito to pyrehtoid compounds have been a common problem in controlling thisdisease among the tropical countries, including Indonesia. Knockdown resistance alleles of voltage-gated sodium channel gene have been proposed as an effective marker for early detection of this problem. This study aimed to understand the pyrethroid resistance status of Aedesaegypti population among Dengue endemic areas in Central Java Province. The house hold larval surveys were conducted in Temanggung, Kendal and Jepara districts and Semarang municipal based on the Dengue cases. Mosquito larvae were reared to be 3-5 days old imago stage in entomologic laboratory, and then subjected to molecular experiment for detection of kdr alleles of domain II VGSC gene. This study found two single nucleotide polymorphisms, namely S989P and V1016G. These SNPs indicated that Aedesaegypti population has developed resistant to pyrethroid compounds. Generally, we found the high percentage of those SNPs, namely 26 and 93 percents. It is an important data input for public health officer in planning of Dengue prevention.
Co-Authors Sayono Sayono Ulfa Nurullita