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Henna Ria Sunoko
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Rice Leaf Extract for Kidney Damage Prevention in Plumbum-Exposed Rats Budi Santosa; Henna Ria Sunoko; Andri Sukeksi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Abstract. Plumbum (Pb) is a heavy metal affecting renal tubular epithelial cells and causing necrosis. This study aimedto demonstrate the effectiveness of rice leaf extract in degeneration and necrosis prevention of renal tubule epitheliumexposed to lead. Twenty eight rats were divided into a control and 3 treatment groups all exposed to Pb by 0.5 g/ kg/ day,but only the treatment groups had received rice leaf extract in varied doses for 8 weeks. Results from Kruskal-Wallis andsphearman rho tests showed that the number of normal cells, degeneration and necrosis were significantly different (0.03;0.05; 0.04) between control and treatment groups with a strong positive correlation of treatment (r = 0.97) in normal cellsand a strong negative one in cell degeneration and necrosis. (R = -0.92 and r = -0.93). As conclusion, rice leaf extractshowed ability in the prevention of kidney damage in rats exposed to lead.
ANALYSIS, IDENTIFICATION, AND FORMULATION OF METALLOTHIONEIN EXTRACTS ON NUMEROUS VARIETIES OF PADDY 95 LEAVES Budi Santosa; Henna Ria Sunoko
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Background: Vegetable materials, such as rice, corn, bean, and soybean have metallothionein proteins contained in their roots, stems, leaves, flowers, and fruits. Of those vegetable materials, paddy leaves have the most numerous metallotionein proteins. However, the varieties which produce the highest metallotionein proteins have not yet been found. The roles of metallothionein proteins are to bind and to detoxify heavy metals. Objectives:  to analyze, identify, and formulate the metallothionein extractscontained in numerous varieties of paddy leaves. An experimental research method. Each vegetable material of numerous varieties of paddy leaves is made into simplicia and is then processed into infusion. ELISA method is used as a technique to measure the protein level of each infusion. The metallothionein protein levels contained in numerous varieties of paddy leaves are then identified to determine the one with the highest metallothionein protein level. Extract formula is taken from the highest metallothionein level. Results :the highest metallothionein protein level is contained in paddy leaves of IR begendit (28.832 ng) while the lowest one is in paddy leaves of IR-64 SS (0.358 ng). The extracts of Paddy leaves of IR begendit with the highest protein level are then formulated into metallothionein extracts. Conclusion: Analysis on metallothionein levels of numerous varieties of paddy leaves with the highest protein level is contained in paddy leaves of IR begendit (28.832 ng) while the lowest one is in paddy leaves of IR-64 SS (0. 358 ng).Keywords: paddy leaves, metallothionein
Co-Authors Andri Sukeksi Budi Santosa