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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Istriyati Istriyati
Laboratorium Embriologi dan Histologi Hewan, Fakultas Biologi Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Analisis Kuantitatif Sel Purkinje Cerebellum Mencit (Mus musculus L.) setelah Induksi Ochratoksin A Selama Periode Organogenesis Setiawan , Arum; Sagi , Mammed ; Asmara , Widya ; Istriyati, Istriyati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.071 KB) | DOI: 10.24002/biota.v16i2.108

Abstract

Penelitian ini bertujuan mengetahui jumlah sel Purkinje cerebellum anak mencit umur 21 hari (pascasapih) setelah induksi Ochratoksin A selama periode organogenesis. Tiga puluh ekor mencit bunting dibagi secara acak menjadi 5 kelompok perlakuan dengan masing-masing 6 ulangan. Ochratoksin A dilarutkan dalam Sodium Bicarbonat, diberikan secara oral pada saat kebuntingan hari ke 7 sampai hari ke -14. Dosis perlakuan Ochratoksin A adalah 0,5 ; 1,0; 1,5 mg/kg bb dan sebagai kontrol tidak diberi perlakuan, serta kontrol placebo diberi perlakuan pelarut Sodium Bicarbonat. Induk mencit dipelihara sampai melahirkan. Pada umur ke 21 hari (pascasapih), anak mencit dikorbankan dan diambil bagian otaknya. Otak mencit selanjutnya dipreparasi dengan metode parafin dan pewarnaan menggunakan pewarnaan Haematoksilin Eosin. Data jumlah sel Purkinje dianalisis dengan Anava Satu Arah dan dilanjutkan dengan uji DMRT untuk mengetahui beda nyata antar perlakuan. Hasil penelitian menunjukkan bahwa Ochratoksin A yang diberikan pada mencit bunting selama periode organogenesis menyebabkan terhambatnya pertumbuhan jumlah sel Purkinje mencit perlakuan yang ditandai dengan semakin menurunnya jumlah sel Purkinje dibandingkan dengan kontrol dan kontrol placebo.
Efek Kurkumin terhadap Pertumbuhan dan Perkembangan Fetus Rattus norvegicus L. Istriyati, Istriyati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i1.2631

Abstract

Curcumin which is isolated from Curcuma longa L. rhizome, has been used as a food additive, medicinal agent, cosmetic and fabric dye, whithout known its side effects. This research is to study the effects of curcumin on the structure of forelimb finger. Twenty virgin female rats, 2 month old, 150 g body weight were mated with fertile male rats. The present of vaginal plug or spermatozoa in the vagina were determined as the first day of pregnancy. Pregnant animals were devided into 4 groups of 5. Treatment were started from 10th to 17th day of pregnancy. The first 2 groups for control and vehicle only. Curcumin was dissolved in aquous 5% Tween 80. The second 2 groups were given curcumin at the dosage of 100 and 150 mg kg -1 bw. Rats were sacrificed at 18th day of pregnancy. Foetuses were dissected out, fixed in 10% formalin. Morphology of the foetuses were observed, forelimb were processed by paraffin method, and plantar paraffin section were made and used for histological observations and cell development of fingers. The results showed that curcumin at the dosage of 100 mg kg-1 body weight caused some foetuses dead, resorption of the embryo, hemorrhagi, unilateral phocomelia. Microscopical observations showed the treatment caused induced of nail formation, inhibition of indensity interdigity, and the increased of new blood vessel formation. It was concluded that the curcumin has no effect on the growth of foetuses. However it was affected the development of forelimb finger which is non dosage dependent.
Co-Authors Arum Setiawan Febri Susilowati, Febri Mammed Sagi Sagi , Mammed Setiawan , Arum Widya Asmara