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Muhammad Noor Jalaluddin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education

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Akumulasi Logam Berat Krom dan Timbal dalam Sedimen Estuaria Sungai Matangpondo Palu Said, Irwan; Jalaluddin, Muhammad Noor; Upe, Ambo; Wahad, Abdul Wahid
Media Eksakta Vol 5, No 2 (2009)
Publisher : Media Eksakta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (12.331 KB)

Abstract

Telah dilakukan penelitian tentang akumulasi logam berat krom dan timbal dalam sedimen estuaria sungai Matangpondo Palu dengan tujuan untuk mendapatkan informasi besarnya konsentrasi logam krom dan timbal yang terdapat dalam sedimen.  Pengambilan contoh dilakukan pada beberapa titik di sekitar muara sungai Matangpondo di Teluk Palu dengan menggunakan corel sample (modifikasi peneliti). Hasil penelitian menunjukkan bahwa keberadaan logam krom dan timbal berasal dari aktivitas alamiah dan aktivitas antropogenik.. Secara parsial kandungan logam berat krom pada salah satu titik contoh telah melampaui batas konsentrasi maksimum yang diperbolehkan (51 mg/kg) dengan rerata 39,24±23,67 mg/kg dan secara holistik belum melampuai NAB tersebut, dengan demikian perairan estuaria sungai Matangpondo masih aman dari pencemaran oleh logam krom. Sedangkan konsentrasi logam timbal dalam sedimen belum melampaui NAB-nya (33 mg/kg) dengan rata-rata 15,89± 7.43 mg/kg.
Pemurnian dan Karakterisasi Enzim Lipase dari Aspergillus oryzae pada Kopra Berjamur Dali, Seniwati Dali; Patong, Abdul Rauf; Jalaluddin, Muhammad Noor; Parenrengi, Pirman Andi
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.336 KB) | DOI: 10.31258/jnat.14.1.26-31

Abstract

An investigation on purification and characterization of lipase enzyme production Aspergillus oryzae from copra by fermentation of oliveoil has been carried out. This enzyme can be produced by fermenting olive oil in a medium containing Aspergillus oryzae. Crude enzyme isobtained by centrifuging the medium cultures containing Aspergillus oryzae at 3500 rpm for 30 minutes and than adding 0.2 M borat buffer(pH 8.2). Enzyme activity was determined from paranitrophenol as product of lipase catalysis of paranitrophenylbutirat (0.2 M) assubstrate measured by the Vorderwulbecke method. Prepurification process was by ammonium sulphate fractionation. Precipitation60-80% ammonium sulphate produced maximum activity of enzyme. Purification by Q sepharosa FF and sephadex G-75 collumchromatography produced four and three fractions with purifity of 12.85 and 20.25 times than crude enzyme respectively. Characterizationof this enzyme showed optimum condition at pH 8.2, temperature at 350C, the Km value at 0.046 M, and Vmaks is 1.926 μmol/menit and themolecular weight at 40.7 kDa.
Co-Authors Abdul Rauf Patong Abdul Wahid Wahad Anak Agung Istri Sri Wiadnyani Irwan Irwan Pirman Andi Parenrengi Seniwati Dali Dali