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Yohanes Armawan Sandi
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Chemistry

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HIDROLISIS RUMPUT LAUT (Glacilaria sp.) MENGGUNAKAN KATALIS ENZIM DAN ASAM UNTUK PEMBUATAN BIOETANOL Yohanes Armawan Sandi; Wiwik Susanah Rita; Yenni Ciawi
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.749 KB) | DOI: 10.24843/JCHEM.2016.v10.i01.p02

Abstract

The aim of this research is to determine the effect of enzyme and acids concentration on the yield of glucose produced in the hydrolysis of Glacilaria sp. in the production of bioethanol. The concentrations of cellulase used were 200 units/mL, 400 units/mL, 600 units/mL, 800 units/mL and the concentration of sulphuric acid (H2SO4) and hydrochloric acid (HCl) used were 1%, 3%, 5%, 7% (w/v). The concentration of reduction sugar was determined using Anthrone and analyzed using UV-Vis spectrophotometry and the determination of ethanol concentration was carried out by using gas chromatography. The results showed that the contents of reducing sugar produced by sulphuric acid (H2SO4) hydrolysis were 26,19%; 36,69%; 41,40%; 45,0% (v/v), by hydrochloric acid (HCl) were 12,12%; 14,03%; 15,17%; 16,50% (v/v), and by cellulase enzyme were 46,15%; 46,73%; 47,68%; 48,25% (v/v). Optimum concentration of reducing sugar produced by hydrolysis using 800 units/mL cellulase was 48,25% (v/v). The optimum length of fermentation to produce bioethanol using Glacilaria sp. as raw material was 5 days. In the fermentation, inoculum with a concentrations of 5% and 10% (w/v) produced 0,85% and 1,51% (v/v) ethanol.
Co-Authors WIWIK SUSANAH RITA Yenni Ciawi