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All Journal Jurnal Kimia (Journal of Chemistry)
I M. Sukadana
Program Studi Kimia FMIPA Universitas Udayana, Jimbaran, Bali, Indonesia
Chemistry

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SENYAWA ANTIMAKAN TERHADAP Epilachna Sparsa DARI BATANG GAYAM (Inocarpus fagiferus Fosb.) I M. Sukadana; S. R. Santi; N. N. T. Hanayanthi
Jurnal Kimia (Journal of Chemistry) Vol.14 No.1 Januari 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (78.751 KB) | DOI: 10.24843/JCHEM.2020.v14.i01.p09

Abstract

Epilachna sparsa is one of the plant pests that damage the epidermal layer on the bottom side of the leaves, so it can lead to damage of leaf tissue and only the skeleton remains. Gayam (Inocarpus fagiferus) is one of the species of the Legumineceae family which has insecticide activity. This study aims to isolate and identify the antifeedant triterpenoid compound from stem of gayam (Inocarpus fagiferus Fosb.) against Epilachna sparsa larvae. Dried powder of 1200 g gayam stems produced 17.82 g of crude methanol extract with antifeedant activity of 60.74% at a concentration of 5% (w/v) which was further separated by partition to obtain n-hexane, ethyl acetate, and water extracts. Extracts of n-hexane, ethyl acetate, and water at 5% (w/v) concentration showed antifeedant activity of 46.74%, 18.09%, and 8.31% respectively. Futhermore, the most active n-hexane extract was identified by phytochemical tests and LC-MS/MS. The phytochemical test showed n-hexane extract contains triterpenoid compounds. The mass spectra from LC-MS/MS chromatogram peaks with MassLynx V4.1 software and Chemspider database for n-hexane extracts which were previously purified by SPE (Solid method) Phase Extraction) using methanol and dichloromethane (DCM) eluents show the presence of components of flavonol pyrogallol, sucrose2,3,3 ', 4', 6-pentaacetate, soyasaponin I, and a compound with a molecular weight of 685,225 g/mol which cannot be identified further because there is no match spectra with the database. Keywords: antifeedant, Epilachna sparsa, gayam, Inocarpus fagiferus Fosb., triterpenoid.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F.) TERHADAP BAKTERI Staphylococcus aureus N. M. Puspawati; N. L. P. F. Widiari; I M. Sukadana
Jurnal Kimia (Journal of Chemistry) Vol.14 No.1 Januari 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.825 KB) | DOI: 10.24843/JCHEM.2020.v14.i01.p10

Abstract

This study aims to examine the antibacterial activity of n-hexane, ethyl acetate, and n-butanol extract of tenggulun leaves (Protium javanicum Burm. F.) in inhibiting the growth of S. aureus bacteria and determine the minimum inhibitory concentration of the most active extracts and identify its active compounds. A total of 1000 g of tenggulun leaf powder was macerated with methanol and 91.78 g of crude methanol extract was obtained which was further partitioned into n-hexane, ethyl acetate, and n-butanol extracts. Antibacterial activity test was carried out using the diffusion well method and the result showed that the ethyl acetate extract at a concentration of 20% was strongly able to inhibit the growth of S. aureus bacteria with inhibition zones of 20.08 mm, followed by n-butanol 16.66 mm, and n-hexane 13.33 mm. The minimum concentration of ethyl acetate extract was 0.2% in inhibiting the growth of S. aureus with inhibition zone diameter of 6.20 mm. Separation of the active ethyl acetate extract was conducted by silica gel column chromatography with mobile phase of n-hexane: ethyl acetate (7.5: 2.5) gave 6 combined fractions. Fractions B, G, H and I showed antibacterial activity at concentrations of 50% with a diameter of inhibition zone of 5.62 mm, 6.87 mm, 8.50 mm, and 6.75 mm respectively. The results of mass spectra analysis from the chromatogram peaks of LC-MS / MS suggested that the G and I fractions were tentatively identified as crotarin, benzophenone-2, and medroxyprogesterone acetate which may contributed to the antibacterial activity by acting synergisticly. Keywords: antibacterial, tenggulun, Staphylococcus aureus, LC-MS/MS.
Co-Authors N. L. P. F. Widiari N. N. T. Hanayanthi Ni Made Puspawati S. R. Santi