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All Journal International Journal of Biosciences and Biotechnology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Planta Tropika Agroteknika Prosiding Seminar Nasional Program Pengabdian Masyarakat Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi
Nike Triwahyuningsih
Department Of Forestry, Faculty Of Forestry, Institut Pertanian INTAN Yogyakarta, Jl. Magelang Km 5,6 Yogyakarta
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Veterinary

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Karakteristik Kimiawi Kompos Enceng Gondok dan Jerami Hasil Dekomposisi Dengan Aktivator Alami dan Buatan Triwahyuningsih, Nike
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 1, No 1 (2005)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v1i1.3109

Abstract

A research to study the chemical properties of hyacinth- and straw-compost decomposed by natural and artificial activators was conducted at the Research Field of Faculty of Agriculture, Muhammadiyah University of Yogyakarta. Cow manure was used as natural activator, while Stardec (powdered) and EM4 (liquid) as artificial ones. The treatment was arranged in 2x3 factorial completely randomized design. The hyacinth and straw organic sources were decomposed by cow manure, Stardec and EM4 activators. Organic matters were incubated for 5-6 weeks then pH, C-organic, N-total, available P and K, CEC, and the .exchngeable  Ca,  K and Mg were measured. The results showed that the hyacinth-compost had the better maturity according to the C/N ratio (C/N 20) than the straw-compost (C/N 20), but the two kinds of compost had the moderate mature rate of humic substances.  The straw-compost had better quality though as shown in its higher available phosphorus and excheangable potassium, especially when decomposed by EM4 artificial activator. Application of artificial activators Stardec and EM4 enhanced the maturity rate of hyacinth-compost.
Kajian Aktivitas Infeksi Rhizobium sp. Indigenus Pada Kerandang (Pueraria phaseoloides) Triwahyuningsih, Nike; Pristiwantoro, Eko Cahyo; Utari, Lilik
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 1, No 2 (2005)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v1i2.3121

Abstract

Kudzu, a kind  of  legume   cover  crops  (LCC),   plays  important   roles  in  soil and  water conservation. It is effective  for  inhibiting  erotion,  compaction  and leaching  of nutrients,  as well as to suppress weeds.  Plants growing with legume  ground covers usually show  better nutrition,   growth and yield. Recently,  this cover crops have been widely  used to prevent  the negative effects  and to improve  soil physical  and nutritional  characteristics. As a legume,  bacterial  inoculation   is to enhance  the infection and nodulation activity. A research  to observe  the role of indigenous  Rhizobium   on infection and nodulation  activity of kudzu was carried  out in September  till December 2004.  The research  was done in Regosol  volcanic  soil in Tlatar  Village  of Sawangan in Magelang district of Central  Java. A pot experiment   was arranged in 4x2 factorial    completely   randomized   design  which  was repeated   three times.   The  seedling   innoculation   with  root  nodules  endophytic   bacteria   was  to increase  nodulation  and to enhance  Kudzu  plant growth.   The seedlings  were inoculated  with  1, 2 and 3 ml/pot of grinded  root nodules  of wild plants  containing  endophytic  Rhizobium  sp.  The wild kudzu  plants were taken  from  coastal  sandy  land  of southern   Yogyakarta.   All treated seedlings were planted  in a sterilized  medium  to be compared  to the unsterilized  one in order to examine  the role of indigenous  bacterias. Observation  on bacterial infection activity, nodulation,  and plant growth were taken  at week  4, 6, 8, and  10. All the  datas  were  variance   analyzed   and the  treatments average  were  tested  with 5% level  of Duncan's multiple range test. The  results  showed  that  application   of 2 ml/pot  of innoculum significantly   increased  the infection  activity, nodulation  and plant growth. Sterilized  soil decreased  the infection  activity.   Since the innoculation  of endophytic Rhizobium  sp.  in unsterilized  soil significantly   increased the infection actifity,  nodulation  and plant growth,  so the indigenous  Rhizobium sp. in rhizhosfer  was needed to sinergisticaly  worked  well with inoculum  to increase  infection  activities   and root nodulation.
ISOLATION, CHARACTERIZATION AND INOCULUM FORMULATION OF NODULE FORMING BACTERIA OF KUDZU (Pueraria phaseoloides (Roxb.)Benth.) FOR COASTAL SANDY LAND CONSERVATION Nike Triwahyuningsih; Tati Budi Kusmiyarti
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.08 KB) | DOI: 10.24843/IJBB.2018.v06.i01.p02

Abstract

Kudzu plantation (Pueraria phaseoloides) as legume cover crop is one of alternatives in coastal sandy land conservation. The crops are known to associate with a root nodule-forming bacteria (Rhizobium sp.) which give some benefits to nutrient cycling i.e. : atmospheric N2 fixing and play role as soil conditioner; soil Nitrogen enrichment; nutrient cycling; and increasing other nutrients availability. A research to study the isolation dan bacterial inoculum multiplication from wild kudzu root nodules, compatible isolates screening and selected isolates multiplication, and examining the form, amounts and most proper inoculum application method was conducted in Greenhouse and Laboratory of Microbiology in Yogyakarta province.The research were held in four phases : (1) isolation, purification and characterization of isolates; (2) reinoculation dan compatibility testing of isolates to kudzu seeds; (3) inoculum multiplication; and (4) examination of the form, amounts and most proper inoculum application method. Physical and biochemical properties of the isolates were observed during the isolation phase. Infection and nodulation activity were observed during the reinoculation phase. Indirectly counting of the microbial numbers to obtain the cell numbers was conducted during the inoculum multiplication. While infection and nodulation activity and plant growth were observed during the inoculum testing phase.Isolates purification on Yeast Mannitol Agar + congo-red media gave 5 different isolates named RP-Etp1, RP-Etp2, RP-Etp3, RP-Etp4, RP-Etp5. The RP-Etp4 isolate had the highest compatibility to the kudzu seeds (number of effective nodules >100 per plant), followed by RP-Etp5 (medium compatibility, number of effective nodules 50–100 per plant), RP-Etp1 and RP-Etp3 (low compatibility, number of effective nodules < 10). Isolate RP-Etp2 was incompatible to the kudzu.Optimum cell numbers was reached in 48 hours incubation time. Application of broth/liquid inoculum of Rhizobium sp. has advantages over solid inoculum (in peat) as it gives the highest number of nodules, and the optimum dosage was 2 – 4 ml per plant. The highest infection-nodulation activity and plant growth were reached in 4 ml inoculum per plant (direct application) or 2 ml inoculum per plant (weekly applied in two weeks).
Karakteristik Kimiawi Kompos Enceng Gondok dan Jerami Hasil Dekomposisi Dengan Aktivator Alami dan Buatan Nike Triwahyuningsih
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 1, No 1 (2005)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v1i1.3109

Abstract

A research to study the chemical properties of hyacinth- and straw-compost decomposed by natural and artificial activators was conducted at the Research Field of Faculty of Agriculture, Muhammadiyah University of Yogyakarta. Cow manure was used as natural activator, while Stardec (powdered) and EM4 (liquid) as artificial ones. The treatment was arranged in 2x3 factorial completely randomized design. The hyacinth and straw organic sources were decomposed by cow manure, Stardec and EM4 activators. Organic matters were incubated for 5-6 weeks then pH, C-organic, N-total, available P and K, CEC, and the .exchngeable  Ca,  K and Mg were measured. The results showed that the hyacinth-compost had the better maturity according to the C/N ratio (C/N20) than the straw-compost (C/N20), but the two kinds of compost had the moderate mature rate of humic substances.  The straw-compost had better quality though as shown in its higher available phosphorus and excheangable potassium, especially when decomposed by EM4 artificial activator. Application of artificial activators Stardec and EM4 enhanced the maturity rate of hyacinth-compost.
Pengembangan “Sekolah Sampah” Dalam Mendukung Program Pendidikan Karakter Peduli Lingkungan (Pendampingan Pendirian Sekolah Pengelolaan Sampah Ar Raihan Bantul) Nike Triwahyuningsih
Prosiding Seminar Nasional Program Pengabdian Masyarakat 2019: 6. Penanggulangan Bencana dan Pelestarian Lingkungan Hidup
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.488 KB) | DOI: 10.18196/ppm.26.527

Abstract

Pendidikan karakter peduli lingkungan, yang bertujuan mencetak generasi peduli lingkungan danproduktif, dilaksanakan melalui pengembangan “Sekolah Pengelolaan Sampah Ar Raihan Bantul”.Sasaran program ini adalah seluruh warga sekolah, pegiat sampah dan masyarakat umum. Pelaksanaankegiatan meliputi: observasi, diskusi kelompok, sosialisasi program, pelatihan/praktik aplikasi teknologi,pendampingan dan monitoring evaluasi. Observasi dan diskusi dengan pengelola yayasan dilakukanuntuk mengidentifikasi potensi dan merumuskan bentuk program, sosialisasi kepada seluruh anggotayayasan dilakukan untuk menyebarluaskan rancangan program. Kegiatan penyuluhan dan pelatihanaplikasi teknologi dilakukan untuk memberikan bekal penguasaan teknologi bagi calon pelatih darisekolah sampah. Praktik aplikasi teknologi bagi warga sekolah telah dilaksanakan mulai Januari-Juli2019 dan akan dilaksanakan secara berkala dengan sasaran lebih luas. Pelaksanaan kegiatanpendidikan, yang dilaksanakan dalam bentuk integrasi program Sekolah Sampah ke dalam kurikulumformal sekolah serta kegiatan ekstra-kurikuler bagi siswa, sudah dimulai pada semester genap 2018-2019.Pendampingan dan monitoring evaluasi dilakukan untuk mengkaji perkembangan pelaksanaan kegiatandan tingkat capaian sasaran. Hasil kegiatan menunjukkan bahwa pendirian dan pengembangan SekolahSampah memperoleh dukungan penuh dari pengurus yayasan dan sekolah. Sekolah Pengelolaan SampahAr Raihan Bantul telah berbadan hukum (Nomor AHU-0002746.AH.01.07.TAHUN 2019).Pendampingan pendirian Sekolah Pengelolaan Sampah ditandai dengan peresmian oleh Bupati Bantulpada 27 Juli 2019.
Inovasi Pengendalian Rayap Kayu Kering (Cryptotermes spp.): Pengujian Multiaspek Biopestisida Nabati Berbasis Ecoenzyme Terhadap Mortalitas, Repelensi, dan Perlindungan Substrat Triwahyuningsih, Nike; Shafira, Amrina Yasmine; Kusmiyarti, Tati Budi; Maulana, Alief Yahya
Agroteknika Vol 8 No 3 (2025): September 2025
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/agroteknika.v8i3.583

Abstract

Rayap kayu kering (Cryptotermes spp.) merupakan hama perusak kayu yang menyebabkan kerugian ekonomi signifikan, dan penggunaan pestisida kimia konvensional menimbulkan masalah lingkungan dan kesehatan. Inovasi penggunaan biopestisida perlu dilakukan untuk meningkatkan efikasi dan stabilitasnya. Sebuah penelitian dilakukan untuk mengevaluasi pengaruh pemberian biopestisida berbasis ecoenzyme terhadap mortalitas, repelensi, dan daya cegah serangan rayap kayu kering. Biopestisida-ecoenzyme dibuat dengan cara memfermentasi 7 bahan nabati sebanyak 12,5% di dalam ecoenzyme selama 30 hari. Pada uji aplikasi biopestisida dan penentuan LC50, percobaan disusun menurut rancangan acak lengkap dua faktor dengan tiga ulangan. Faktor pertama adalah cara pemberian (uji mortalitas, uji repelensi, dan uji proteksi) dan faktor kedua adalah dosis biopestisida (0, 10, 20, 30, 50, dan 75%). Hasil penelitian menunjukkan bahwa biopestisida-ecoenzyme secara signifikan efektif dalam mengendalikan populasi rayap kayu kering melalui tiga mekanisme utama, yaitu: 1) bio-pestisida mampu menyebabkan kematian pada rayap kayu kering dengan nilai LC50 5,90%; 2) biopestisida mampu mengusir rayap kayu kering dengan nilai LC50 35,77%; 3) biopestisida mampu mencegah serangan rayap kayu kering dengan nilai LC50 56,97%. Dengan demikian biopestisida berbasis ecoenzyme memiliki potensi besar sebagai agen pengendali hama rayap kayu kering yang efektif. Pada uji frekuensi pemberian biopestisida, percobaan disusun menurut rancangan acak lengkap dua faktor dengan tiga ulangan. Faktor pertama adalah dosis biopestisida (0, 6, 36, dan 57%) dan faktor kedua adalah frekuensi pemberian (1, 2, dan 3 minggu sekali). Hasil pengujian frekuensi pemberian biopestisida menunjukkan mortalitas rayap sangat tinggi (lebih dari 80%) dengan aplikasi hanya setiap 3 minggu sekali menggunakan konsentrasi 6%.
Pengolahan Sampah Organik Sebagai Upaya Peningkatan Ketahanan Iklim Masyarakat Triwahyuningsih, Nike; Shafira, Amrina Yasmine; Tati Budi Kusmiyarti
ABDIKAN: Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Vol. 4 No. 3 (2025): Agustus 2025
Publisher : Yayasan Literasi Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55123/abdikan.v4i3.6171

Abstract

Community empowerment activities aimed at enhancing the knowledge and skills of the community in managing and processing household organic waste have been conducted in Notoprajan Village, Yogyakarta City. The target of this activity is the PKK members and community groups in the village. The activity was conducted using extension and education methods on waste management, which included waste reduction and handling, followed by practical training on processing organic waste. Through this activity, products have been produced in the form of Ecoenzyme scrub, used cooking oil candles, gkalranulated compost, pasteurized liquid organic fertilizer, tutorial videos, and publications in national journals. It is concluded that this training activity can enhance the knowledge and skills of the residents of Kalurahan Notoprajan in managing and processing organic waste. The impact of the success of this activity is contributing to the direct reduction of organic waste volume, minimizing methane (CH4) production and other greenhouse gas emissions, supporting climate change mitigation efforts at the community level, providing practical skills that can open new economic opportunities for the community, transforming waste into sellable products, and enhancing financial independence, as well as improving climate resilience through increased environmental awareness, independence, and active community participation in maintaining the cleanliness and health of their own environment
COMPOSITION, STRUCTURE, AND CARBON SEQUESTRATION OF DIFFERENT RAINFOREST ECOSYSTEMS IN THE GUNUNG GEDE PANGRANGO NATIONAL PARK, INDONESIA Sunyata, Agus; Rakhmi Zulhida, Isna; Triwahyuningsih, Nike; Raharja, Kanda
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.2042

Abstract

ARTICLE HIGLIGHTS- High demand for environmental services makes the park vulnerable to human activities.- Both ecosystems are well regenerated; seedling > sapling > pole > tree (inverted J)- Both ecosystems show normal diversity conditions and stable species distribution.- Growth of Maesopsis eminii needs monitoring to preserve forest purity.- Montane forests have greater biomass, carbon stocks, less anthropogenic disturbanceABSTRACTThe Gunung Gede Pangrango National Park (GGPNP) area is one of the vital ecosystems that support the environment in West Java Province, Indonesia. It is a unique area that has multiple forest ecosystems, including lowland rainforest and montane rainforest ecosystems. Despite the GGPNP’s status as a conservation area, the high demand for the GGPNP’s environmental services makes the region vulnerable to disturbances from human activities. Several studies have been conducted in the GGPNP area (lowland and montane forest ecosystems), however, the results of this study are still necessary to explain the forest dynamics and forest carbon sequestration in this location. The objective of this research was to analyze the structure, composition, and carbon sequestration of stands in the lowland and montane rainforest ecosystems in the GGPNP area. Data processing and analyses were conducted using diversity indices, biomass-carbon stock estimation, and carbon dioxide sequestration estimation. The results showed that the GGPNP lowland and montane rainforest ecosystems were well regenerated. The number of seedlings > saplings > poles > trees and the graph showed a reverse “J” pattern. The GGPNP lowland rainforest ecosystem was dominated by Neonauclea lanceolata and had relatively higher species diversity. The GGPNP montane rainforest ecosystem was dominated by Castanopsis acuminatissima with a higher individual density, denser canopy, and more complex canopy strata. The lack of regeneration in several species of trees heightens the threat to these species’ existence in the future. Biomass, carbon stocks, and carbon sequestration in the GGPNP montane rainforest were greater than those in the GGPNP lowland rainforest. The GGPNP montane rainforest ecosystem had older forest stands, a larger average tree diameter, and lower potential for anthropogenic disturbances.
Co-Authors Maulana, Alief Yahya Pristiwantoro, Eko Cahyo Raharja, Kanda Rakhmi Zulhida, Isna Shafira, Amrina Yasmine Sunyata, Agus Tati Budi Kusmiyarti Utari, Lilik