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All Journal International Journal of Biosciences and Biotechnology
Winny Dewi Widarmi
Corteva Agriscience (PT Dupont Agricultural Product Indonesia), Cibis Nine 10th floor, Jl. TB Simatupang No. 2, East Cilandak, South Jakarta 12560, Indonesia
Biochemistry, Genetics & Molecular Biology Environmental Science

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CYTOLOGICAL VARIATION OF (AAG)7 REPEAT ON LETTUCE CHROMOSOMES BY FLUORESCENCE IN SITU HYBRIDIZATION (FISH) Winny Dewi Widarmi; Shinji Kikuchi; Hidenori Sassa; Takato Koba
International Journal of Biosciences and Biotechnology Vol 8 No 2 (2021): International Journal of Bioscience and Biotechnology
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2021.v08.i02.p06

Abstract

Cytological studies using fluorescence in situ hybridization (FISH) technique provides phylogenetical information in closely related taxa and have been widely applied for karyotyping and studying chromosomal organization and evolution in plant species. In the present study, FISH using a microsatellite sequence of (AAG)7 as the probe was performed in order to discriminate the chromosomes in four Lactuca species, i.e., L. sativa, L. serriola, L. saligna, and L. virosa. The experiment was carried out in April to September 2018 at Laboratory of Genetic and Plant Breeding of Breeding of Graduate School of Horticulture, Chiba University, Japan. Different distribution patterns of (AAG)n signals were shown on the chromosomes in the four Lactuca species studied, In L. sativa and L. serriola, FISH with (AAG)7 sequences revealed dispersed distribution patterns with one pair of bright signals, respectively. While in L. saligna and L. virosa, distinct signals with different intensities were observed in two pairs of chromosomes of L. saligna and five pairs of chromosomes of L. virosa. In conclusion, the AAG repeat signals could be used as cytogenetic landmarks for chromosome identification in Lactuca species.
Co-Authors Hidenori Sassa Shinji Kikuchi Takato Koba