Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal International Journal of Biosciences and Biotechnology
Lia Kusumaningrum
Chemistry Department, Faculty of Mathematics and Natural Science, Udayana University
Biochemistry, Genetics & Molecular Biology Environmental Science

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
ISOLATION OF PROTEASE ENZYME FROM CHAYOTE FRUIT (Sechium edule (Jacq.) Sw.) WITH AMMONIUM SULFATE FRACTINATION METHOD Ketut Ratnayani; Lia Kusumaningrum
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.676 KB)

Abstract

Protease is an enzyme that is capable to hydrolyze (breakdown) protein molecules intosimpler compounds such as small peptides and amino acids. The aim of the research was toisolate protease enzyme from chayote (Sechium edule (Jacq.) Sw.) using fractinationammonium sulfate method and to find out the optimum saturation level of the ammoniumsulfate. P rotease activity examination of each fraction of ammonium sulfate was performedusing Anson method. Protein content assay was determined using Biuret method. The resultsshowed that crude extract protease of chayote had specific activity of 3,7338 x 10-3 U/mg. Theoptimal saturation levels of ammonium sulfate for protease chayote precipitation was 40-50%. At this saturation level, the highest enzyme spesific activity were 16,00 x 10-3 U/mg,with four times purifying of protease enzyme from the crude extract protease.
Co-Authors Ketut Ratnayani