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Ketut Wella Mellisandy
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Identifikasi Spesies Stafilokokus Pada Ikan Kerapu Di Kabupaten Karangasem Dengan Analisis Sekuen 16S rRNA (SPECIES IDENTIFICATION OF STAPHYLOCOCCUS ON GROUPER IN KARANGASEM REGENCY BASED ON 16S RIBOSOMAL RNA SEQUENCE ANALYSIS) I Nengah Kerta Besung; Ketut Wella Mellisandy; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study aims to identify the species Staphylococcus grouper in Karangasem Regency.Faeces samples were collected and grown on media Blood order and identified by Gram staining.Suspected staphylococcal bacterial culture, included in the 10% Chelex media. The 16S rRNAgene fragments amplified from DNA (deoxyribonucleic acid) by Polymerase Chain Reaction(PCR) with specific primers that is Tsta G422 and Tstag 765. PCR (Polymerase Chain Reaction)products were sequenced in Barkley Sequencing Facility in the USA The result of sequencing wasedit and sorted using MEGA 5.0. Final sequence from each isolate was aligned using database in GenBank by fitur of BLAST. Several of standard sequences were downloaded to construct thefilogenetik tree. Species which can be confimed was Staphylococcus warneriwith homology valueof 100% with the data in GenBank.
Co-Authors I Gusti Ngurah Kade Mahardika