Andrianus Sembiring
Biodiversitas Indonesia (Bionesia), Denpasar, Bali – Indonesia

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Identifikasi dan Keragaman Genetik Longtail Tuna (Thunnus tonggol) Yang Didaratkan di PPI Kedonganan dan PPP Muncar Menggunakan Marka D-loop Mitokondria Enex Yuniarti Ningsih; Elok Faiqoh; Ida Ayu Astarini; Putu Dian Pertiwi; Andrianus Sembiring; Ni Luh Astria Yusmalinda; Muhammad Danie Al Malik
Journal of Marine and Aquatic Sciences Vol 7 No 1 (2021)
Publisher : Fakultas Kelautan dan Perikanan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/jmas.2021.v07.i01.p13

Abstract

Longtail tuna (Thunnus tonggol) is one of the oceanodromus neritic species and the migration pattern follows the water currents. Currently, this species has not been widely studied in Indonesian waters, so it is nesessary to study the identification of morphology and genetic diversity. The molecular approach employed in this study is DNA barcoding using mitochondrial D – loop locus. This study can explain the importance of species genetic information in stability and resilience. This study aims to determine the identification of morphology, phylogenetic and genetic diversity of longtail tuna at two locations in PPI Kedonganan, Bali dan PPP Muncar, Banyuwangi. The molecular analysis was done in several stages, i.e DNA extraction, Polymerase Chain Reaction, electrophoresis and sequencing. Based on the result of sequencing and analysis, 33 samples of longtail tuna was found. The result of phylogenetic tree reconstruction from two locations showed one clade with genetic distance value among longtail tuna species ranging from 0.000 – 0.042 for all close kinship samples. The haplotype diversity (Hd) value of longtail tuna was 0.9905 and nucleotide diversity (?) was 0.020. The high value of genetic diversity indicated that two longtail tuna populations have a high survival ability to adapt on environmental changes. Index fixation analysis (Fst) has a value of 0.0299, p - value > 0.05. The index fixation value indicated no significant population difference. The result of this study can be use as basic data in planning genetic conservation strategies with sustainable fisheries management efforts. Keywords: Tuna; Thunnus tonggol; Genetic Marker
Keragaman Genetik Ikan Tongkol Abu-Abu (Thunnus tonggol) yang Didaratkan di Pasar Ikan Sagulung, Batam, Kepulauan Riau Berdasarkan DNA Mitokondria Suryany Devy; Ida Ayu Astarini; I Nyoman Giri Putra; Andrianus Sembiring; Luh Astria Yusmalinda; M. Danie Al Malik; Ni Putu Dian Pertiwi
Journal of Marine and Aquatic Sciences Vol 7 No 2 (2021)
Publisher : Fakultas Kelautan dan Perikanan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/jmas.2021.v07.i02.p06

Abstract

Longtail Tuna (Thunnus tonggol) is one of the neritic species of tuna from the Scrombridae family. T. tonggol is oceanicromus and ussually found in tropical and subtropical waters in the Indo-Pacific region. Although known as one of the tuna species, the information of this species is very lacking, especially in Indonesia. Therefore, this study aims to determine the genetic diversity and kinship relationships of longtail tuna (T. tonggol) collected at Sagulung fish market, Batam, Riau Islands. Molecular analysis were carried out using genetic markers namely mitochondrial D-loop (control regian) with the stages of DNA extraction, PCR (Polymerase Chain Reaction), electrophoresis, sequencing, and data analysis. A total of 21 longtail tuna samples were identified using molecularly and confirmed as T. tonggol species, which have a base length ranging from 482 - 523 bp (base pairs). The value of haplotype diversity (Hd) of longtail tuna was 1,00000 and the value of nucleotide diversity (?) was 0,01654. The results of phylogenetic tree reconstruction showed that all samples collected were in the same group (clade) with the average genetic distance in one T. tonggol clade obtained a value of 0.010 which has a close relationship between samples. This result can be use as additional information for longtail tuna species in Indonesia.