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Christy Priskila Kairupan
Universitas Sam Ratulangi

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UJI DAYA HAMBAT EKSTRAK ETANOL DAUN KEMBANG SEPATU (Hibiscus rosa-sinensis L) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli Kairupan, Christy Priskila
PHARMACON Vol 3, No 2 (2014)
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/pha.3.2014.4779

Abstract

The prevalence of diarhea in Indonesia is considered high and one cause is Escherichia coli. The aim of this research was to determine anti-bacterial inhibition, effective concentration, and the effect of increasing concentration of ethanol extract of Hibiscus rosa-sinensis to the growth of E. coli. Extract of H. rosa-sinensis was obtained by masseration using 95% ethanol as solvent. Bacterial growth inhibition employed Kirby-Bauer Method using agar diffusion through wells. Measurement of bacterial inhibition was analysed using one way anova and continued with Least Significantly Difference. Anova showed inhibition by 5%, 10%, 20%, 40%, 80% of extracts. Effective concentrations occured on 20% and 40%. The increasing of extract concentration showed correlation with the increasing of inhibition zone diameters.   Key words : Hibiscus rosa-sinensis leaf, ethanol extract, inhibition, E. coli