Ketut Dharma Susila
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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PHOSPHATASE ACTIVITY AND PHOSPHATE SOLUBILITY BY PHOSPHATE SOLUBILIZING RHIZOBACTERIA IN VOLCANIC SOILS OF PANCASARI, BALI Ketut Dharma Susila; I Made Sudana; Ni Putu Ristiati; I Made Adnyana
International Journal of Biosciences and Biotechnology Vol 4 No 1 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Phosphatase in the soil was found as extracellular enzymes produced by soil micro-organisms both in acidic or alkaline conditions. Phosphatase is an enzyme complex that plays an important role in deciding soil-bound phosphate bond organic compounds to form orthophosphate which is available to the plant. Mineralization of organic forms into a inorganic-P determined by the ability of these bacteria to produce phosphatase. Therefore, observations of phosphatase activities are important to know how intensely P mineralization process takes place in the soil. Phosphate solubilizing rhizobacteria (PSR) has been widely known to affect mobilization of insoluble inorganic phosphates become available to plants. Although phosphate solubilizing rhizobacteria is widely available in the soil, their activity is usually not strong enough to compete with other bacteria commonly located in the root zone (rhizosphere). That is why, screening of phosphatase enzyme activity and various phosphate solubilizing ability to obtain better strains of bacteria are still needed. This study was conducted to determine the ability of phosphate solubilizing rhizobacteria, its potential as a biofertilizer inoculant and characterize its capacity to dissolve P-insoluble inorganic and produce phosphatase enzymes in the growing medium. Potential phosphate solubilizing rhizobacteria was tested for their ability in vitro using Pikovskaya media containing insoluble forms of inorganic phosphate as a source P. The results of this study indicate that there are some isolates phosphate solubilizing rhizobacteria that are capable of dissolving the insoluble inorganic-P with different abilities. The highest potential successively displayed by isolates TbPP-4.1; BdPP-2.1; and SBPP-1.3 that significantly has the same ability to soluble inorganic phosphate in the soil. The isolates BdPP-2.1 and TbPP-4.1 are significantly higher than the other isolates for producing phosphatase. Both isolates TbPP-4.1 and BdPP-2.1 have the same pontenty as a biofertilizer based on its capacity to mineralize forms of soil organic phosphorus by phosphatase activity.
PENERAPAN INOVASI TEKNOLOGI LUBANG RESAPAN BIOPORI UNTUK MENJAGA EKOSISTEM TANAH DAERAH RAWAN BANJIR DI BANJAR WIRASATYA, DENPASAR SELATAN Tatiek Kusmawati; Tati Budi Kusmiyarti; M.Tri Gunasih; I B. P. Bhayunagiri; K.Dharma Susila; N. W. Sri Sutari
Buletin Udayana Mengabdi Vol 11 No 1 (2012): Volume 11 No.1 – April 2012
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Our planet is heating up. We need to do something to avoid a total collapse of environment and our life. The climate changes, have been felt by us in Indonesia, and also happened in the earth side of the world. The effect has come continuously with uncertain time and spread all over the Indonesian territory along 2006 up to 2007 only.The real fact that often meet in our general society, no matter with what that happen around us, the most important thing is we can do some activities as usual, and can buy everything that we want and feel fresh, that all. We also often forget the disaster happening quickly. The global climate changest caused by global warming requires us to change life`s habit that does not care to avoid the rate of global warming. It can be done by illumination and training activities to improve the understanding of that condition and also develop the awareness of the society, especially to introduce Biopori Leach Holes technology done in Banjar Wirasatya Suwung Sidakarya. Biopori leach holes are appropriate technology and environmentally friendly way to cope with flooding (1) improve water infiltration, (2)convert organic waste into compost and reduce emissions of greenhouse gases (CO2 and methane), and (3) utilize the role of activity soil fauna and plant roots, and overcome the problems caused by inactive water such as dengue fever and malaria.
Pengaruh Pupuk Hayati dan Pupuk Anorganik Terhadap Beberapa Sifat Kimia Tanah Serta Hasil Tanaman Bayam Cabut (Amaranthus tricolor) di Tanah Inceptisol Desa Pedungan SEINA RIZKY PRIAMBODO; KETUT DHARMA SUSILA; NI NENGAH SONIARI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.1, Januari 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Effect of Biofertilizers and Anorganic Fertilizers to Several Soil Chemical Properties and Spinach (Amaranthus tricolor) in Pedungan Village Inceptisol The aim of this study is to know the effect of interaction between biofertilizers and inorganic fertilizers on soil chemical properties and yield of spinach in Pedungan Inceptisol. This research was conducted from October 2017 to February 2018 at the greenhouse. The experiment located in Pedungan Village and the Soil and Environmental Laboratory, Agriculture Faculty of Udayana University. Randomized Block Design (RBD) with 9 treatments and 3 replications were used in this experiment. The data were analyzed by using the Costat program and continued by the LSD test at 5% level if the treatment was significantly different. The variables observed in this study consisted of soil chemical properties and production of plants. The result of this experiment showed that there are no interaction between biofretilizer and inorganic fertilizer on all of parameters observed. The effect of biofertilizers had a significant diferences on all of parameters, while in using inorganic fertilizers only on the half parameters. The treatment of biofertilizers at a dose of 20 mL had a significant effect on the improvement on all parameters, while inorganic fertilizer treatment at half of the recommended dose had the same effect as the treatment of real recomended dose for increasing the total N, K, soil pH, and plant height.
Evaluasi Status Kesuburan Tanah Pada Lahan Pertanian di Kecamatan Denpasar Selatan I DEWA AYU SRI PURNAMI PINATIH; TATI BUDI KUSMIYARTI; KETUT DHARMA SUSILA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Evaluation of Soil Fertility Status At Agriculture Land in South Of Denpasar District This study aims to evaluate the status of soil fertility in South Denpasar District. The method used in the research is soil sampling survey with started making land unit acquired by overlaying several thematic maps, then conducted some soil sampling individuals for each land unit who then composited. Soil samples have been obtained and analyzed in the laboratory analysis of soil chemical properties include: 1). Cation Exchange Capacity (CEC), 2). Base saturation (BS), 3). Soil Organic Matter, 4). Total Soil Phosphorus, 5).Total Soil Potasium. The results analysis of soil fertility status chemical characteristic are then clasified of soil fertility status based on Bogor Soil Research Center Prosedures (1995). The results showed that in South Denpasar District there are two classes of soil fertility status of medium fertility class (S) which includes land unit DS1 (Subak Renon, Intaran, Sidakarya, Wangbiga), DS2 (Subak Suwung), DS3 (Sanur Kauh) while high soil fertility is also found on the land unit DS1 sample DS1d . Two parameters of soil fertility which is the limiting factor is the low chriteria of soil organic matter status and total soil phosporus. Alternative of management land to address both the limiting factors are the addition of organic matters and phosphorus fertilization. Keywords: soil fertility status, South  of Denpasar district, agriculture land
Evaluasi Kualitas Tanah di Lahan Sawah Simantri dan Non Simantri di Subak Riang Desa Riang Gede, Kecamatan Penebel NI LUH AYU PADMAWATI; I DEWA MADE ARTHAGAMA; KETUT DHARMA SUSILA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.2, April 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Soil Quality Evaluation of Paddy Soil Under “Simantri’s” and Non “Simantri’s” System in Subak Riang, Riang Gede Village, Penebel Subdistrict This experiment conducted to evaluate the differences between soil quality of paddy soil sunder “Simantri’s” and “non Simantri’s” activities, as well as to evaluate the differences among major physical, chemical and biological soil properties which determining their quality. There were three steps applied in this study including: field survey to determine the research area and sampling points; Minimum Data Set to determine the soil quality and t-test to determine the dominant factor which influencing the soil quality. The results of this study showed: the soil quality of faddy soil under “Simantri’s”activity is better than“non Simantri’s” conditions. Other Than That, it was found the soil total nitrogen significantly higher under “Simantri’s” than “non Simantri’s” conditions.
Evaluasi Status Kesuburan Tanah untuk Lahan Pertanian di Kecamatan Denpasar Timur DYAH AYU GEDE WIDYANTARI; KETUT DHARMA SUSILA; TATIEK KUSMAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Evaluation of Soil Fertility Status for Agricultural Land at East Denpasar District   Research on Evaluation of Soil Fertility Status for Agricultural Land in East Denpasar District was conducted in January-July 2014. The purpose of this study was to analyze some soil chemical properties that become obstacles soil fertility status, evaluate and map the soil fertility status of the study area. The method used in this study include, methods of survey and soil testing in the Soil and Environment Laboratory, Faculty of Agriculture, Udayana University. The study begins with  the manufacture of the land unit map by clicking the map overlay-type of soil; map slope; and land use maps. Retrieved five land units and each of land units were taken the composite soil samples and analyzed its chemical properties in the laboratory which includes CEC; KB; C-organic; total – P and K of soil. Further evaluation of soil fertility status corresponding technical instructions of Soil Research Center, Bogor (1995).           The results showed that the soil in the study had two soil fertility status, namely the status of medium and low soil fertility. The location study which has the status of soil fertility was covering land unit T1a; T1b; and T1c at Padang Galak Subak Kesiman Kertalangu (Kesiman Village, Kesiman Kertalangu Village, and Kesiman Petilan Village); T1d on Subak Yeh Lauh (Penatih Village), T3 land unit (Kesiman Kertalangu Village and Penatih Dangin Puri Village), and land units T4 on Subak Semaga (Penatih Village), While the research sites that have low fertility status covering, land unit T2 on Subak Delod Sema (Kesiman Village) and T5 land unit (Penatih Dangin Puri Village). Parameters of soil fertility is a constraint in dermining the fertility status of the soil, in the low content of  C-organic and total-P of soils. Management referral of soil fertility for the land unit T2 and T5 to suggest the addition of organic matter and fertilizer P to improve soil fertility status. Key Words : Soil fertility status; land units; agricultural land; East Denpasar District  
Perbedaan Umur Tanaman Penghijauan Terhadap Perubahan Kualitas Tanah di Desa Pelaga, Kecamatan Petang, Badung JUWITA ROHAYATI MARBUN; KETUT DHARMA SUSILA; I NYOMAN SUNARTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Differences of Greening Planting Ages Against Soil Quality Changes in Pelaga Village, Petang District, BadungLand as an environmental component must be maintained, protected and avoided from adverse impacts and must be maintained and enhanced. This study aims to determine the effect of different age of reforestation plants on soil quality changes in Pelaga Village, Petang District, Badung Regency. The method used in this research is observation method with direct observation in field and laboratory analysis. The research was conducted in accordance with several stages, namely the planning and preparation of the initial survey of reforestation land that aims to determine the point of sampling in accordance with the age of reforestation, soil sampling, analysis of physical properties, chemistry, and biology in the laboratory and analysis of research data. The results showed that there was no change in soil quality on land with different plantation age 1 year, 2 years, 3 years, compared with control (land with grass vegetation) and decreased soil quality was observed on land with planting age 4 years and 5 years (after harvest).
Pemetaan Potensi dan Status Kerusakan Tanah Lahan Pertanian Kecamatan Buleleng, Kabupaten Buleleng EDUART NIKO SIAHAAN; KETUT DHARMA SUSILA; IDA BAGUS PUTU BHAYUNAGIRI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.4, Oktober 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Mapping of Potential and Status of Damage to Agricultural Land in Buleleng District, Buleleng Regency Increased biomass production activities can result in soil damage due to a decrease in soil quality and function that can threaten the continuity of human life. The purpose of this study is to determine the potential for soil damage, determine the status of soil damage, and make maps of the potential and status of land damage. The research was conducted in Buleleng District using scoring methods and overlaying of potential soil damage, the parameters analyzed were land use, soil type, slope and rainfall. Determination of the status of soil damage based on the procedure for measuring the standard criteria for soil damage. The parameters analyzed were content weight, solum depth, surface rockness, fraction composition, weight content, total porosity, permeability, pH, DHL, and the number of microbes. In the study area there are 9 homogeneous land units (HLU). Based on the research results obtained 2 classes of potential soil damage, namely low potential soil with a score of 19-24 and moderate with a score of 25-32. Areas in Buleleng District that have low potential for soil damage are found in SLH I, III, V, VI, and IX covering an area of ??1856.12ha (67.13%), and the potential for moderate land damage is in SLH II, IV, VII and VIII covering an area of?? 908.68 ha (32.87%). The status of soil damage in Buleleng District was lightly damaged with a score of 4. The limiting factor for the study area was permeability. These parameters get a relative frequency value of 88.89% with a score of four. Areas in Buleleng District that have low potential for soil damage are found in SLH I, III, V, VI, and IX covering an area of ??1856.12ha (67.13%), and the potential for moderate land damage is in SLH II, IV, VII and VIII covering an area of 908.68 ha (32.87%). The status of soil damage in Buleleng District was lightly damaged with a score of 4. The limiting factor for the study area was permeability. These parameters get a relative frequency value of 88.89% with a score of four.
Uji Efektivitas Jenis Dekomposer pada Proses Pengomposan Sampah Organik di Kota Denpasar TAUFIQ WARDANA; KETUT DHARMA SUSILA; I WAYAN NARKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.1, Januari 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Test the Effectiveness Some Kinds of Decomposers on the Organic Waste Composting Process in Denpasar City This study aims to determine the quality of compost and decomposition rate of compost mix raw materials by using several kinds of decomposers. This research was conducted from November 2020 to February 2021 at the Sari Sedana Garbage Depot, on Bung Tomo IV Street number 1 in Denpasar, and for the analysis of compost samples located at the Soil Laboratory Agricultural Faculty of Udayana University. The design used in this study was a randomized block design. The compost mix raw materials (CM) consisted of 40% organic waste, 40% cow dung, 10 % husk charcoal, 8 % sawdust, 2 % dolomite, and decomposers (according to each treatment) + molasses. There were six treatments of various decomposers with four replications consisting of P0 (control): 100% organic waste (15 kg), P1: CM + EM4 and molasses, P2: CM + Beka and molasses, P3: CM + BA-5 and molasses, P4: CM + Biofit and molasses, P5: CM + Biogenic and molasses. The observed compost quality parameters included: C-organic, N-total, C/N ratio, P-available, K-available, moisture content, and pH. Data were analyzed using analysis of variance (ANOVA), followed by the Duncan’s Multiple Range Test 5% difference test. The results of the research showed that the use of decomposers had a very significant effect on the quality of the compost produced and composting process rate. The rate of decomposition and the best compost quality according to SNI 19-7030-2004 were shown by the P1 treatment with the EM4 as a decomposer.
Studi Keharaan Tanaman dan Evaluasi Kesuburan Tanah di Lahan Pertanaman Jeruk Desa Cenggiling, Kecamatan Kuta Selatan KETUT DHARMA SUSILA
Agrotrop : Journal on Agriculture Science Vol 3 No 2 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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Study on Plant Nutrient and Soil Fertility Evaluation in Citrus Plantation Land at CenggilingVillage, South Kuta District. The objective of the research to elaborate some soil chemical propertiesand classify soil fertility status of citrus plantation lands at Cenggiling Village, South Kuta District. Soilsurvey method and quantitative measurement of some soil fertility elements in laboratory based onBogor Soil Research Center procedure (1995) was done to determined criteria and soil fertility status.The result of study showed that C1 location at “subak abian” Tanijaya have chemical properties namely:CEC; base saturation; P205; K20 and C-organic are 26.13 me/100g (high criteria); 89.18% (highcriteria); 44.20 mg/100g (high criteria); 57.80 mg/100g (high criteria) and 2.51% (medium criteria)respectively with high soil fertility status. On the other hand, C2 location at “subak abian” Selondinghave chemical properties namely: CEC; base saturation; P205; K20 and C-organic are 23.40 me/100g(medium criteria); 79.36% (high criteria); 35.28 mg/100g (medium criteria); 56.35 mg/100g (highcriteria) and 1.66% (low criteria) respectively with low soil fertility status. The last, C3 location at“subak abian” Puluk-puluk have chemical properties namely: CEC; base saturation; P205; K20 and Corganicare 17.80 me/100g (medium criteria); 87.05% (high criteria); 29.37mg/100g (medium criteria);57.91 mg/100g (high criteria) and 1.54% (low criteria) respectively with low soil fertility status.