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Karakteristik Bakteri di Perairan Mangrove Pesisir Kraton Pasuruan (Characterization of Bacteria Isolated from Mangrove Coastal Waters of Kraton, Pasuruan) Yahya Yahya; Happy Nursyam; Yenny Risjani; Seomarno Soemarno
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 19, No 1 (2014): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.945 KB) | DOI: 10.14710/ik.ijms.19.1.35-42

Abstract

Bakteri memainkan peran penting dalam ekosistem mangrove terutama dalam mengurai searasah daun. Keberadaan bakteri ini sangat dipengaruhi oleh berbagai faktor lingkungan. Penelitian ini bertujuan untuk mengisolasi dan karakterisasi bakteri dari perairan mangrove dari dengan vegetasi yang berbeda yakni Avicennia marina, Rhizophora apiculata, Avicennia alba dan Sonneratia alba. Pengambilan sampel dilakukan di perairan mangrove desa Bulu Kerto Kraton Pasuruan. Proses karakterisasi bakteri dilakukan di laboratorium mikrobiologi Fakultas Kedokteran Universitas Brawijaya Malang. Metode penelitian yang digunakan adalah eksploratif sedangkan parameter uji adalah isolasi bakteri dan uji morfologi, biokimia, serta deteksi pertumbuhan bakteri yang dominan. Hasil penelitian diperoleh 7 isolat bakteri Avicennia marina yaitu Nitrococcus sp., B. subtilis, B. pumilus, Pseudomonas putida. P.stutzeri, Micrococcus luteus, Vibrio sp, 6 isolat bakteri Rhizophora apiculata yaitu B. megaterium, Nitrococcus sp, Staphylococcus sp., P.putida, Lactobacillus sp., B. Subtilis,  8 isolat bakteri Avicennia alba yaitu B.megaterium, B.pumilus, B. subtilis, Nitrococcus sp., Pseudomonas putida, P. stutzeri, B. mycoides, Micrococcus sp.. dan 6 isolat bakteri di Sonneratia alba yaitu Bacillus megaterium, Nitrococcus sp., B.subtilis, Planococcus citreus, B. mycoides, Lactobacillus plantarum. Hasil ini menunjukkan bahwa jenis bakteri dipengaruhi oleh vegetasi mangrove yang ada di suatu perairan. Kata kunci: bakteri, perairan mangrove, vegetasi Bacteria play an important role in the ecosystem, especially in degrading mangrove leaves (litter). The presence of these bacteria is greatly influenced by various environmental factors. This research aims to isolate and characterized bacteria from mangrove waters with different vegetaion namely Avicennia marina, Rhizophora apiculata, Avicennia alba and Sonneratia alba. Characterization of the bacteria were done at microbiological laboratory in Medical Faculty, Brawijaya University. Several tests were done in this explorative research i.e. morphological test, biochemistry, and bacteria growth. The results showed there were 7 isolated bacterias found at Avicennia marina area i.e. Nitrococcus sp, B. subtilis, B. pumilus, Pseudomonas putida. P. stutzeri, Micrococcus luteus, Vibrio sp.; 6 isolated bacterias found at  Rhizophora apiculata area i.e. B. megaterium, Nitrococcus sp, Staphylococcus sp., P. putida, Lactobacillus sp., B. subtilis; 8  isolaed bacterias from Avicennia alba area i.e. B. megaterium, B. pumilus, B. subtilis, Nitrococcus sp., Pseudomonas putida, P. stutzeri, B. mycoides, Micrococcus sp., and 6 isolated bacterias from Sonneratia alba area i.e. Bacillus megaterium, Nitrococcus sp, B. subtilis, Planococcus citreus, B. mycoides, Lactobacillus plantarum. These results indicate that the type of bacteria is influenced by the existing mangrove vegetation. Keywords: bacteria, mangrove waters,vegetation
Peningkatan Kapasitas Produksi Terasi Rebon di Desa Ketapang, Kotamadya Probolinggo Muhamad Firdaus; Candra Adi Intyas; Yahya Yahya
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 6 No 3 (2021): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v6i3.1832

Abstract

Kademangan is a shrimp paste production center in the Probolinggo municipality. The shrimp paste made from this fish processing center is of lower quality than similar products on the market. The varying size of the raw materials is a significant problem in shrimp paste production in this business hub. This activity aimed to improve the quality of the shrimp paste product by introducing and enhancing the partners' skills in using grinders and shrimp powder for rebon shrimp in making rebon shrimp paste. The method of this service activity consists of training, monitoring, and evaluation of the activities. This activity showed that the partners had received training on the production of shrimp paste and its quality and the use of grinding machines and machines for boiling shrimp meal. During the partner monitoring period, it became apparent that the partners had switched the technology from manual production of shrimp paste to machine production. The evaluation results show that the partners are familiar with grinding and grinding machines in making Rebon shrimp paste. The resulting products are of higher quality than those made before the partners used the shrimp grinding shrimp flour machines. The conclusion is that the use of grinding and flour machines for rebon shrimp by partners has increased the production capacity by three times and increased the quality of the rebon shrimp paste.
Fortifikasi Tepung Eucheuma cottonii pada Pembuatan Mie Kering Sebagai Makanan Halal dan Thoyib Muhamad Firdaus; Abdul A. Jaziri; D S Sari; Yahya Yahya; Asep A Prihanto
Indonesia Journal of Halal Vol 1 (2) 2018
Publisher : Pusat Kajian Halal Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.848 KB) | DOI: 10.14710/halal.v1i2.3667

Abstract

Permintaan makanan halal dan thoyib semakin meningkat di dunia. Mie kering adalah salah satu makanan yang sangat digemari masyarakat. Namun, masih ada beberapa produk mie kering yang ditengarahi mengandung bahan baku tidak halal, khususnya mie impor. Selain itu, mie kering memiliki nilai indeks glikemik tinggi, yang kurang aman dan berisiko terhadap kesehatan dan juga rendah akan kandungan yodium. Oleh karena itu, diperlukan fortifikasi dengan rumput laut (E. cottonii). E. cottonii memiliki kandungan karagenan yang sangat besar sehingga kaya akan serat. Serat memainkan peranan penting untuk menurunkan nilai indeks glikemik. Di sisi lain, E. cottonii mengandung yodium yang tinggi. Fortifikasi E. cottonii dalam mie kering diharapkan juga dapat menambah nilai fungsional. Tujuan penelitian ini adalah untuk mengetahui pengaruh berbagai konsentrasi fortifikasi tepung E. cottonii pada indeks glikemik dan gizi mie kering. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan rancangan acak lengkap (RAL). Variabel bebas dalam penelitian ini adalah konsentrasi fortifikasi tepung rumput laut pada adonan mie kering yaitu 5%; 10%; dan 15%. Variabel dependen adalah indeks glikemik, kadar air, kadar lemak, kadar protein, kadar abu, kadar karbohidrat, kadar serat kasar, kandungan yodium, kehilangan masak, tarik, rasa, aroma, warna dan tekstur. Hasil penelitian ini menunjukkan bahwa perlakuan terbaik pada mie kering yang diperkaya dengan 15% tepung E. cottonii, yang terdiri dari 36,14 indeks glikemik dan 45,64 ppm yodium. Adapun kandungan gizi mie kering meliputi 8,95% kadar air, 0,27% lemak, 13,54% protein, 5,72% abu, 71,52% karbohidrat, dan 9,17% serat kasar. Selain itu, karakteristik dari mie kering 4,17% dari cooking loss dan 0,37 N dari kekuatan tarik. Selain itu, analisis organoleptik mie kering adalah 2,7 warna, 2,4 aroma, 2,5 tekstur, dan 2,70 rasa. Nilai indeks glikemik dalam penelitian ini sangat penting untuk menentukan mie kering sebagai makanan halal dan thayyib karena nilai tinggi indeks glikemik dalam makanan akan menyebabkan lonjakan kuat dalam gula darah, peningkatan risiko diabetes tipe 2, penyakit jantung dan kelebihan berat badan. Oleh karena itu, kami simpulkan bahwa formulasi terbaik untuk mie kering dengan 15% E. cottonii sebagai makanan halal dan thoyib. Kata kunci: Mie kering, E. cottonii, fortifikasi, halal, thoyib AbstractThe demand for halal and thayyib food is increasing in the world. Dried noodle is one of favorite food. However, some dried noodles contained non halal ingredients, especially imported noodles. In addition, dried noodles have high glycemic index, which are less safe and risky to health and also low in iodine content. Therefore, we focus to fortify with seaweed (E. cottonii). E. cottonii has  a number of carrageenan content and rich in fiber. Fiber plays an important role to lower the glycemic index. On the other hand, E. cottonii contains high iodine. Fortification of E. cottonii in dried noodle is also expected to add functional value. The purpose of this study was to determine the effect of various concentrations of E. cottonii flour fortification on glycemic index and nutrition of dreid noodle. The method used in this research is an experimental method with complete randomized design (RAL). The independent variable in this research is the concentration of seaweed flour fortification in dried noodle that is 5%; 10%; and 15%. Dependent variables are glycemic index, proximate composition, iodine content, and sensory analysis. The results of this study indicate that the best treatment of dried noodles enriched with 15% E. cottonii flour, consisting of 36.14 glycemic index and 45.64 ppm of iodine. The nutrient content of dry noodles include 8.95% moisture, 0.27% fat, 13.54% protein, 5.72% ash, 71.52% carbohydrate, and 9.17% crude fiber. In addition, the characteristics of dry noodle was 4.17% of cooking loss and 0.37 N of tensile strength. In addition, the organoleptic analysis of dry noodles was 2.7 colors, 2.4 aromas, 2.5 textures, and 2.70 flavors. The value of the glycemic index in this study is crucial for determining dried noodles as halal and thayyib food because the high value of the glycemic index in food will cause a strong spike in blood sugar, an increased risk of type 2 diabetes, heart disease and overweight. Therefore, we conclude that the best formulation for dry noodles with 15% E. cottonii as halal and thayyib food. Keywords: Dreid noodle, E. cottonii, fortification, halal, thayyib