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PRODUKSI BIOHIDROGEN DARI HIDROLISAT AMPAS TAHU SECARA FERMENTASI ANAEROB MENGGUNAKAN KULTUR CAMPURAN Amir Husin; Sarto Sarto; Siti Syamsiah; Imam Prasetyo
Reaktor Volume 15, No.2, OKTOBER 2014
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (599.85 KB) | DOI: 10.14710/reaktor.15.2.87-96

Abstract

Ampas tahu merupakan produk samping industri pengolahan tahu berbahan dasar kacang kedelai (Glysine max.).  Dalam studi ini, ampas tahu yang telah mengalami perlakuan-awal dengan metode yang berbeda dikonversi menjadi hidrogen menggunakan kultur campuran.  Pengaruh perlakuan-awal asam satu-tahap (0,5% dan 10% berat HCl) dan dua-tahap (tahap I 0,5%  dan tahap II 10% berat HCl) terhadap sakarifikasi ampas tahu juga diinvestigasi.  Lebih lanjut, pengaruh perlakuan awal asam satu-tahap maupun dua-tahap terhadap produksi hidrogen dan efisiensi degradasi gula tereduksi dibandingkan.  Yield total gula-tereduksi 503,73 mg/g ampas tahu diperoleh dari perlakuan-awal asam dua-tahap.  Nilai ini kurang lebih 4 kali lebih tinggi dibanding hasil dari perlakuan-awal asam satu-tahap menggunakan 0,5% berat HCl. Studi produksi biohidrogen dilakukan secara batch menggunakan kultur campuran dengan kondisi reaksi  35oC dan pH awal 6,5,  Hasil percobaan menunjukkan, bahwa yield hidrogen kumulatif  maksimum 0,928  mmol/g  ampas tahu diperoleh dari perlakuan  asam dua-tahap  atau meningkat 1,8 kali dibanding perlakuan satu-tahap menggunakan 0,5% berat HCl. Key words : ampas tahu,  perlakuan asam, produksi hidrogen Abstract Tofu solid waste is one of the by-products of tofu-processing industry.  In this study, batch experiments were carried out to convert tofu solid waste pretreated by different methods into hydrogen using mixed culture.  The effects of one-stage (0.5% or 10% HCl) and two-stage (first stage 0.5% HCl) and second stage 10% HCl) pretreatments on the saccharification of tofu solid waste were also studied.  Furthermore, the effects of  and/or  two- stages acid pretreatments on hydrogen production and degradation efficiencies the reducing-sugar (RS) were compared.  A maximum total RS yield of 503.73 mg/g-tofu solid waste was obtained from substrate pretreated with two-stages method.  It was approximately 4-fold greater than that from substrate pretreated with one-stage method using 0.5% wt HCl. At the reaction condition of 35oC, initial pH =  6.5, and RS concentration based on 2 grams of tofu solid waste pretreated, a maximum cumulative hydrogen yield was 0.928  mmol/g-tofu solid waste from substrate  pretreated  with  two-stages method.  It was approximately 1.8-fold greater than that from substrate pretreated with one-stage method using 0.5% wt HCl. The results show that two-stage acid preteatment can enhancing the amount of  reducing sugar in the mixture and hydrogen yield from tofu solid wastes. 
PENGARUH HYDRAULIC RETENTION TIME PADA PRODUKSI BIOHIDROGEN DARI SAMPAH BUAH MELON (Cucumis melo L.) MENGGUNAKAN REAKTOR ALIR PIPA SECARA KONTINYU Nurkholis Nurkholis; Sarto Sarto; Muslikhin Hidayat
Jurnal Inovasi Teknik Kimia Vol 1, No 2 (2016)
Publisher : Fakultas Teknik Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31942/inteka.v1i2.1652

Abstract

Penelitian ini bertujuan untuk mengevaluasi pengaruh hydraulic retention time (HRT) terhadap laju produksi dan yield bio-H2 dari sampah buah melon secara kontinyu menggunakan reaktor alir pipa, termasuk juga produksi asam-asam organik sebagai hasil samping (by-product.. Penelitian dilakukan dalam 2 tahap, yaitu pra-perlakuan dan pengkayaan inokulum campuran sludge dari biodigester sampah buah, tahu dan kotoran sapi dengan menggunakan HCl 37% 2M (acid treatment) dan tahap proses produksi H2. Proses produksi H2 dilakukan secara fermentasi gelap, dengan terlebih dahulu dilakukan aklimatisasi (start-up) mikroorganisme yang terlibat dalam proses fermentasi gelap. Proses pengumpanan substrat selanjutnya dilakukan dengan variasi HRT 7 hari, 5 hari dan 3 hari secara bertahap dalam waktu 21 hari. Sampel gas dan cairan diambil dari reaktor untuk dilakukan analisis kadar H2, volatile solid (VS) dan volatile fatty acid (VFA). Dari hasil penelitian dapat disimpulkan bahwa, laju produksi dan yield bio-H2 optimal pada HRT yang singkat yaitu 3 hari, dimana secara berturut-turut mencapai  224,8587 mL dan 50,4097 mL/ g VS, sedangkan konsentrasi VFA tertinggi mencapai 47.000 mg/ L pada HRT 7 hari, dimana asam asetat merupakan konstituten domian. Hal ini dapat menyebabkan inhibisi yang dapat menurunkan produksi gas. Kata kunci: bio-H2, sampah buah melon, hydraulic retention time (HRT).
Emisi Gas Metana dan Karbon Dioksida pada Proses Pengolahan Limbah Cair Pabrik Pengolahan Kelapa Sawit PT. Bio Nusantara Teknologi Bengkulu Tengah Linarsih Makmun; Sarto Sarto
Berita Kedokteran Masyarakat (BKM) Vol 34, No 3 (2018)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.123 KB) | DOI: 10.22146/bkm.28299

Abstract

Emission of methane and carbon dioxide gas in the process of processing oil palm liquid waste PurposeThe purpose of this study was to know, calculate and evaluate the concentration of methane gas and carbon dioxide produced in each wastewater treatment plant (WWTP) pond.MethodThis research was a qualitative research, using case study design and explanatory approach. The object of this research was the emission of methane gas and carbon dioxide emitted from 12 WWTP ponds.ResultsThe highest COD and BOD decrement occurred in pond 4 of 39% COD and 61.2% BOD. The highest total methane gas emission was 1.49 x 109 kg hours-1 (1.49 x 106 tons hour-1) occurring in the morning, while the highest total carbon dioxide emission was 2.59 x 109 kg per hour (2.59 x 106 ton hour-1). ConclusionThe concentrations of methane and carbon dioxide gas produced by each WWTP pool varied greatly depending on temperature, residence time and the amount of mud. Methane gas emissions and carbon dioxide emissions occurred in each WWTP pool with the highest methane gas emission value occurring in pond 3 in the afternoon at 356,64 x 106 mg m-2minute-1 and the highest carbon dioxide emissions occur in pond 3 in the afternoon at 402.145 x 106 mg m-2minute-1. The decrease of COD value in whole anaerobic pool was 52,1% and the decrease of COD value in aerobic pool was 27,2%.
Analisis risiko kesehatan akibat pajanan timbal (Pb) dalam biota laut pada masyarakat sekitar Teluk Kendari Muhamad Syarifuddin; Sarto Sarto
Berita Kedokteran Masyarakat (BKM) Vol 34, No 10 (2018)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.582 KB) | DOI: 10.22146/bkm.33933

Abstract

Analysis of health risk due to lead (Pb) exposure in marine biota on the community around Kendari BayPurpose: To understand the risk level of health problem because of the lead exposure found on fish and shellfish that was consumed by community around Kendari bay.Methods: This study was an analytical observational through a cross sectional study design by combining Environmental Health Risk Analysis (ARKL) and Environmental Health Epidemiology (EKL) approaches. Population around Kendari bay was taken using proportional sampling criteria. The marine biotas in this study were 30 fish samples of 3 kind of most consumed fish and shellfish from 6 fishponds in Kendari bay. Measurement of lead content on it was made by Atomic Absorption Spectrometry (AAS).Results: The results of the study shows the lead content on fish and shellfish were 0,0027-0,0095 mg/kg and 0,1026-0,1097 mg/kg respectively while intake rapidity of fish and shellfish 0,0051 mg/kg/day and 0,016 mg/kg/day respectively. Health risk level of consuming contaminated fish was 1.29 while the contaminated shellfish was 4.03.Conclusions: The community around Kendari Bay has a risk of health problems (RQ> 1) due to lead exposure, therefore it needs to be controlled.
Anaerob-aerob biofilter evaluation in general hospital of NTB Nurfitria Hariyani; Sarto Sarto
Berita Kedokteran Masyarakat (BKM) Vol 34, No 5 (2018)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.903 KB) | DOI: 10.22146/bkm.35092

Abstract

Evaluating the use of anaerobic-aerobic biofilter to increase the quality of hospital wastewater Purpose: This study aimed to evaluate the anaerobe-aerobic biofilter systems effectiveness to treating parameters that are affected by the existing units. Methods: This study was conducted with quasi-experimental pretest-posttest approach. Sampling was done in peak hour, intermediate, and minimum wastewater discharge The data analysis used a comparison of quality standards and used the effectiveness level of wastewater treatment through the established formula. Results: The results showed that anaerobic-aerobic biofilter was highly effective in treating total coliform at peak discharge (99.9%), intermediate discharge (83.80%), minimum discharge (99.9%) and effective in processing COD parameters 70.13% at intermediate, and 74.60% minimum discharge. The results also showed anaerobic-aerobic biofilter was quite effective in treating BOD (54.59%) and TSS (50%) and less effective phosphate (40.7%). Data analysis showed that the results of wastewater treatment of provincial hospital in West Nusa Tenggara have met the established quality standards. Conclusion: An anaerobic-aerobic biofilter is effective to increase hospital wastewater to reduce environmental pollution. The impact of this processing will reduce environmental pollution.AbstrakTujuan: Penelitian bertujuan untuk mengevaluasi penggunaan sistem biofilter anaerob-aerob terkait efektivitas dalam mengolah parameter yang dipengaruhi oleh unit yang ada. Metode: Penelitian ini merupakan penelitian quasi experimental dengan menggunakan pendekatan pre-test dan post-test. Pengambilan sampel dilakukan pada saat debit puncak, menengah, dan minimum air limbah. Analisis data menggunakan perbandingan baku mutu dan menggunakan tingkat efektivitas IPAL melalui rumus yang telah ditetapkan. Hasil: Hasil penelitian menunjukkan biofilter anaerob-aerob sangat efektif mengolah total koliform pada debit puncak (99,9%), debit menengah (83,80%), debit minimum (99,9%) dan efektif dalam mengolah parameter COD 70,13% pada debit menengah, dan 74,60% debit minimum. Hasil penelitian menunjukkan biofilter anaerob-aerob cukup efektif dalam mengolah BOD (54,59%) dan TSS (50%) dan kurang efektif mengolah fosfat (40,7%). Analisis data menunjukkan bahwa hasil pengolahan air limbah rumah sakit provinsi NTB telah memenuhi baku mutu yang telah ditetapkan. Simpulan: Biofilter anaerob-aerob efektif meningkatkan kualitas air limbah rumah sakit. Dampak dari proses pengolahan ini akan  mengurangi pencemaran lingkungan.