Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Kedokteran Brawijaya Medical Journal of Indonesia
Setyawati S. Karyono
Laboratorium Farmakologi FK Unibraw Malang
Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Prevention of endothelial dysfunction in streptozotocin-induced diabetic rats by Sargassum echinocarpum extract Firdaus, Muhamad; Astawan, Made; Muchtadi, Deddy; Wresdiyati, Tutik; Waspadji, Sarwono; Karyono, Setyawati S.
Medical Journal of Indonesia Vol 19, No 1 (2010): February
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.838 KB) | DOI: 10.13181/mji.v19i1.382

Abstract

Aim This study aimed to elicit the protective effect of Sargassum echinocarpum extract on endothelial dysfunction in thoracic aorta of streptozotocin-induced diabetic rats.Methods The animals were divided into 5 groups. The first was normal, the second was diabetic non treated animals. The third to fifth groups were the diabetic animals which given Sargassum echinocarpum extract (150; 300, and 450 mg kg-1 body weight, respectively) by oral gavage and extract treatment was given for 12 weeks. Diabetes was induced by single administration of streptozotocin (45 mg kg-1, i.p.), dissolved in freshly prepared 0.1 M citrate buffer, pH 4.5. Diabetes was confirmed ten days latter in streptozotocin induced animals showing blood glucose levels > 200 mg dL-1 (11.1 mmol L-1) as monitored in the blood from tail vein using glucometer. After the treatment period, the blood serum acquired was used for antioxidant enzymes assays and the thoracic aorta was used for vasorelaxation assay.Results There was a significant decrease in the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) in diabetic rats (3.31 ± 0.12;67.17 ± 0.62;35.10 ± 0.83) comaped to control rats (9.97 ± 0.12;185.31 ± 0.23;116.38 ± 0.88). Administration of Sargassum extract increased the activity of SOD, CAT, and GSH-px. The diabetic rats exhibit endothelial dysfunction as shown by loss of vasodilatory response to acethylcholine (ACH). This was restored by administration of Sargassum extract.Conclusion Sargassum echinocarpum extract ameliorates oxidative stress and reverses the endothelial dysfunction associated with diabetes. This effect appears to be due to its antioxidant properties. (Med J Indones 2010; 19:32-5)Keywords: oxidative stress, sargassum echinocarpum, endothelium dependent relaxation, thoracic aorta
EFEK EKSTRAK DAUN CIPLUKAN (Physalis minima L) TERHADAP RELAKSASI OTOT POLOS TERPISAH TRAKEA MARMUT (Cavia porcellus) Priyantoro, Sigit Tri Yus; Sudjari, Sudjari; Karyono, Setyawati S.
Jurnal Kedokteran Brawijaya Vol 20, No 1 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.636 KB) | DOI: 10.21776/ub.jkb.2004.020.01.7

Abstract

Physalis minima L’s leaf contains atropin and skopolamin which possess anti cholinergic effect. Cholinergic agent has constriction effect to the smooth muscle of respiratory tract. In asthma bronchiale there is an airway constriction. This experiment was to observe the effect of Physalis minima L’s ethanol extract on the relaxation of the guinea pig’s trachea’s smooth muscle which was induced with histamin by the method of in vitro. Isolated trachea of 5 male guinea pig’s was used as samples in this experiment. Transducer, amplifier and mac lab computer were used to create  the trachea’s contraction–relaxation curve. The independent variabel was Physalis minima L’s leaf ethanol extract dosage andthe dependent variabel was the trachea’s contraction. 4 dosage of Physalis minima L’s leaf ethanol extract were used (1 control, 3 comparable dosage), the dosage of 0.5 % Physalis minima L’s leaf extract showed significant relaxation of trachea (anova, p<0.05). There was a positive corelation between theincreasing dosage of Physalis minima L’s leaf ethanol extract and the relaxation of trachea (regression, r = 0.770, p< 0.05). The conclusion of this study is that the Physalis minima L’s leaf ethanol extract has positive effect in relaxing isolated trachea of guinea pig’s induced by histamin and there is a positive correlation between increasing dosageof Physalis minima L’s leaf ethanol extract and the relaxation of isolated trachea. Keywords:trachea smooth muscle’s relaxation, histamine, Physalis minima L’s leaf ethanol extract.
Co-Authors DEDDY MUCHTADI MADE ASTAWAN Muhamad Firdaus Sarwono Waspadji Sigit Tri Yus Priyantoro Sudjari Sudjari Tutik Wresdiyati