A K Kataria
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Assessment of Oxidative Stress in Peste des petits ruminants (Ovine rinderpest) Affected Goats A K Kataria; N Kataria
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.247 KB) | DOI: 10.5398/medpet.2012.35.3.170

Abstract

The aim of the present investigation was to evaluate oxidative stress in goats affected with peste des petits ruminants (PPR). The experiment was designed to collect blood samples from PPR affected as well as healthy goats during a series of PPR outbreaks which occurred during February to April 2012 in different districts of Rajasthan state (India). Out of total 202 goats of various age groups and of both the sexes, 155 goats were PPR affected and 47 were healthy. Oxidative stress was evaluated by determining various serum biomarkers viz. vitamin A, vitamin C, vitamin E, glutathione, catalase, superoxide dismutase, glutathione reductase and xanthine oxidase, the mean values of which were 1.71±0.09 µmol L-1, 13.02±0.14 µmol L-1, 2.22±0.09 µmol L-1, 3.03±0.07 µmol L-1, 135.12±8.10 kU L-1, 289.13±8.00 kU L-1, 6.11± 0.06 kU L-1 and 98.12±3.12 mU L-1, respectively. Each parameter analysis of variance showed highly significant effect (P=0.0001) of health status and age category.  Further interaction between health status and age category was also highly significant (P=0.0001) for each parameter studied. The results indicated that vitamins A, C, E and glutathione levels depressed by 18.95%, 38.67%, 47.64%, and 47.39%, respectively and the serum catalase, superoxide dismutase, glutathione reductase and xanthine oxidase activities increased by 90.79%, 75.11%, 90.34%, and 44.06%, respectively in affected animals as compared to that in healthy ones. On the basis of the altered levels of serum biomarkers of oxidative stress it was concluded that the animals affected with PPR developed oxidative stress.
Evaluation of Antimicrobial Efficacy Against E. coli Isolates of Different Serogroups Obtained from Diarrhoeic Neonates of Calves, Kids, and Lambs R Pachaury; A K Kataria
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.859 KB) | DOI: 10.5398/medpet.2012.35.3.175

Abstract

Fourteen antimicrobials were evaluated their efficacy against 115 Escherichia coli isolates recovered from faecal samples collected from neonatal diarrhoeic calves (66), kids (25) and lambs (24).  Of these isolates 37 were nontypeable, four were of rough type and 74 isolates were typeable for 'O' antigen belonging to 19 different serogroups (O2, O3, O5, O17, O21, O22, O25, O41, O45, O55, O60, O65, O70, O76, O114, O116, O147, O152, and O158). Evaluation of antimicrobial efficacy by disc diffusion method revealed that the most effective antibiotics were ciprofloxacin, sparfloxacin and triple sulpha followed by co-trimoxazole, chloramphenicol, nitrofurantoin, gentamicin, nalidixic acid, polymixin-B and amoxyclav in decreasing order of their efficacy while ampicillin and cephotaxime were found totally ineffective against these isolates. All the isolates showed multiple drug resistance to various antibiotics ranging from two to ten antibiotics. The resistance patterns were independent of serogroups of isolates and the animal species from which isolates were obtained. However, there was a variation in resistance pattern for same antibiotic vis-a vis locations of their isolation.