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Supargiyono Supargiyono
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Cell mediated immunity in malaria : 1. Changes in numbers of mononuclear phagocytes during plasmodium vinckei petteri infection in immunized and non-immunized mice Supargiyono Supargiyono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Penelitian bertahap telah dilakukan'untuk mempelajari reaksi-reaksi immunitas selular yang terjadi selama infeksi Plasmodium vinckei petteri malaria pada mencit LACA. Fokus kajian pada penelitian tahap pertama ini adalah mempelajari profil perubahan jumlah sel fagosit mononuklear (mononuclear phagocytes) dalam sumsum tulang, darah tepi dan dalam limpa, selama infeksi malaria pada mencit yang diimunisasi dan yang tidak diimunisasi. Imunisasi dilakukan dengan penyuntikan secara intravena 108 parasit stadium darah yang dibebaskan dari eritrosit dengan larutan saponin dan difiksasi dengan larutan formalin. Kedua kelompok mencit, yang diimunisasi dan yang tidak diimunisasi diinfeksi dengan 103 eritrosit terinfeksi, pada hari ke 15 sesudah imunisasi. Jumlah sel fagosit berinti satu beserta sel prekursornya dievaluasi berturut-turut pada hari ke 0, 3, 6, 9 dan 12 sesudah infeksi. Hasil penelitian menunjukkan bahwa selama infeksi P. v. petteri, jumlah sel fagosit mononuklear meningkat di dalam sumsum tulang, kemudian di dalam darah tepi dan dalam limpa. Derajat peningkatan jumlah sel fagosit tersebut lebih tinggi pada mencit yang telah diimunisasi sebelumnya dibandingkan dengan pada mencit yang tidak diimunisasi. Tingginya peningkatan jumlah sel fagosit mononuklear pada mencit yang diimunisasi tersebut nampaknya berkaitan dengan keberhasilan sistem kekebalan tubuh hospes dalam menurunkan parasitemia. Peningkatan jumlah sel fagosit tersebut didahului dengan peningkatan jumlah sel prekusornya di dalam sumsum tulang, sehingga bisa diantisipasikan bahwa produksi dan proliferasi dari sel prekursor di dalam sumsum tulang juga meningkat dalam usaha memenuhi kebutuhan sel d.ewasa di dalam darah dan jaringan selama infeksi malaria.Key words: Plasmodium vinckei petteri -- malaria -- LACA mice -- mononuclear phagocyte -- cellular immunity
Protective immure responses against fatal malarial infection in mice with blood stage malaria vaccin. Supargiyono Supargiyono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 26, No 03 (1994)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Studies were carried out to establish suitable infection and immunization method to facilitate investigation on the protective host immune responses against acute malarial infection. Combinations of experimental model using two species of rodent malaria parasites Plasmodium vinckei vinckei and Plasmodium vinckei petteri, two immunization methods were tested, to determine which model are suitable for studying any aspect of host immune responses during protective malarial immunization.The results indicated that the infection of P. v. vinckei and P. v. petteri in LACA or in BALB/c mice generally produce acute and lethal infections. Infection of P.v.vinckei in these experimental models seems more severe than P. v. petteri, and neither intravenous nor intraperitoneal immunization gave significant protection against homologous infection. However, immunization using 108 blood stage parasite P. v. petteri could protect LACA mice from the lethality of homologous challenge, and it seems that cell mediated mechanisms play an important role in host responses against infection. Therefore, P. v. petteri infection in LACA mice and immunization using 10 blood stage parasites given intravenously or intraperitoneally represent a suitable model to study some aspects of host immune response mechanism during malarial immunization.Key Words : Plasmodium vinckei vinckei - Plasmodium vinckei petteri - immune response - cellular immunity - homologous parasites
Pengaruh Asap Obat Nyamuk Bakar Terhadap Lama Hidup, Kernampu.an Bertelur, Daya Tetas Telur dan Daya Tetas Larva Aedes aegypti Supargiyono Supargiyono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 20, No 02 (1988)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Mosquito incense coil had been widely use among the households of Indonesian community as an effort to prevent mosquito bites. Nevertheless, its definite influence to the mosquito's biology is still not yet known.This study was conducted to ensure the influence of mosquito incense coil smoke to the longevity, egg production capability, egg hetching capacity and instar emerging capacity of Aedes aegypli mosquito in Yogyakarta.Aedes aegypli mosquitos were collected either from urban or rural area, reared in the laboratory and prepared for laboratory testing. Incense burning was done in a closed-testing room, in six different periods from 5 minutes to 180 minutes. Some biological aspects such as longevity, egg production, egg hetching and instar emerging were observed.It can be concluded that mosquito's incense smoke has either chase out effect or killing effect to Ae. aegypti mosquitos. It is also clear that with the incense burner period of 30 minutes will prolong the longevity, reduce egg production capability, reduce egg hetching capacity and reduce instar emerging capacity. On the incense burning period of 120 minutes, 81.7% of Ae. aegypti tested were killed, and furthermore, in the incense burning period of 180 minutes, all Ae. aegypti tested were killed.Key Words: egg production capability -- egg hetching capacity — instar emerging capacity chase out effect — mosquito incence coil
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