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. Suswardana
dr. mintohardjo Navy Hospital, Jakarta
Immunology & microbiology Neuroscience

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The effect povidone-iodine on the wound healing process: A study on fibroblast populated collagen lattice (FPCL) model Retno Danarti; . Suswardana; Arief Budiyanto; Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.34 KB) | DOI: 10.19106/JMedScie.004603201401

Abstract

Povidone-iodine (PI) 10% solution is an effective antiseptic. However, it appears to be toxic tothe cells involved in wound healing. The aim of this study is to evaluate the toxicity of PI oncultured human fibroblast using fibroblast populated collagen lattice (FPCL) model. The culturedhuman fibroblast was divided into 6 groups i.e. 5 groups were exposed by PI 1, 0.1, 0.01, 0.001and 0.0001%, and 1 group was exposed by phosphate-buffered-saline (PBS). Twenty-four hourslater, the media was washed using PBS. The size of the FPCL media on each group wasobserved over time by serial photographs, which then were measured by Image-J computerprogram. Exposure of 0.1, 0.01, 0.001 and 0.0001 PI caused an obvious reduction of fibroblast’scontraction capability on FPCL media, which described temporary fibroblast injury, that showinga concentration-dependent recovery phenomenon after 48th hour. Furthermore, 1% PI exposureleads to a permanent fibroblast injury. In conclusion, PI exposure in concentration more than0.1% has a permanent toxic effect on fibroblast that clearly observed using a simple FPCLmodel.
Co-Authors Arief Budiyanto Yohanes Widodo Wirohadidjojo