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All Journal International Journal of Public Health Science (IJPHS) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of the Medical Sciences (Berkala Ilmu Kedokteran) INDONESIAN JOURNAL OF PHARMACY Journal of Community Empowerment for Health
Hakim, Mohamad Saifudin
Department Of Microbiology, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Codon Optimization and Epitope Identification of L1 Protein Human Papillomavirus Type 52 for Expression in Lactococcus lactis: Codon Optimization and Epitope Identification L1 Protein HPV52 Prastyowati, Anika; Hakim, Mohamad Saifudin; Wijayanti, Nastiti; Wibawa, Tri
Journal of Tropical Life Science Vol. 15 No. 3
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.03.03

Abstract

Human papillomavirus (HPV) type 52 is among the top three high-risk oncogenic types that are associated with cervical cancer worldwide, especially in Indonesia. Designing a vaccine against HPV using an Lactococcus lactis expression system is a promising strategy, as this bacterium is well-known for its probiotic properties and can be safely utilized as a delivery carrier for oral vaccines. This study aimed to optimize the codon usage of the L1 protein of HPV type 52 for expression in L. lactis expression system and to identify potential epitopes using bioinformatics tools. To determine the codon optimization, the L1 protein sequence of HPV type 52 was retrieved from the National Center for Biotechnology Information (NCBI) database. Furthermore, codon optimization was conducted once the model had been established using OPTIMIZER, Clustal Omega, Restriction Mapper, and the ExPASy tool, based on L. lactis. The final construct was predicted to have a B-cell epitope by Ellipro tools, antigenicity by VaxiJen v.2.0, allergenicity by AllerTOP v.2.0, and toxicity by ToxIBTL. This resulted in a potential vaccine candidate with a Codon Adaptation Index (CAI) of 0.754 and GC content of 39.0%. Identification of epitopes from the optimized gene resulted in two peptide sequences, WRPSEATVYLPPVPVSKVVS and GTLGDPVPGDLYIKGSNSGNTATVQ, as components of the vaccine candidate.
Co-Authors Agit S. Adisetiadi Anika Prastyowati, Anika Edwin W. Daniwijaya Eggi Arguni Fitra Duhita Iryna Dzyublyk Luthvia Annisa Maria E. Pranasakti Maryia Leleka Mumtihana Muchlis Mumtihana Muchlis Nastiti Wijayanti Oktaviarum S. Utama Rakhmat Ari Wibowo Rita Dian Pratiwi Rita Dian Pratiwi Serhii Soloviov Siswanto Siswanto Supriyati Titik Nuryastuti Tri Wibawa Viktor Trokhymchuk Yati Soenarto