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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Indonesian Journal of Biotechnology Pharmacon Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Paediatrica Indonesiana Jurnal Surya Medika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Majalah Farmaseutik JSFK (Jurnal Sains Farmasi & Klinis) PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ilmu Kefarmasian Indonesia Jurnal Mandala Pharmacon Indonesia ad-Dawaa : Journal of Pharmaceutical Sciences Indonesian Journal of Hospital Administration JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Ahmad Dahlan Medical Journal Berita Kedokteran Masyarakat Mandala of Health : A Scientific Journal Journal of Clinical Microbiology and Infectious Diseases Indonesian Journal of Biomedicine and Clinical Sciences Indonesian Journal of Pharmacology and Therapy
Titik Nuryastuti
Departemen Mikrobiologi, Fakultas Kedokteran, Kesehatan Masyarakat Dan Keperawatan (FKKMK), Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Current in vitro assay to determine bacterial biofilm formation of clinical isolates Nuryastuti, Titik
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.978 KB) | DOI: 10.19106/JMedScie004603201406

Abstract

Biofilm mode of growth is bacterial lifestyle widespread in microbial world and represents a concernin health care. Recently, serious problem concerning biofilm-related infection is increasing significantly.However complexity of biofilm makes it difficult to conduct proper analysis. Although biofilm representsa major challenge for microbiologist, methods aimed to determine biofilm formation and developmentare not standardized yet. The aim of the present review was to provide an overview of the advantagesand disadvantages of the difference in vitro methods for determining biofilm forming ability ofclinical isolates.
High Frequency Spontaneous Deletions within the IcaADBC Operon of Clinical Staphylococcus epidermidis Isolates. Nuryastuti, Titik; van der Mei, Henny C.; Busscher, Henk J.; Kuijer, Roel; Aman, Abu Tholib; P. Krom, Bastiaan
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.791 KB)

Abstract

Staphylococcus epidermidis has been shown to undergo a phase variation correlating with expression of the icaADBC operon which contributes to biofilm formation. Biofilm formation of Enterococcus faecalis is related to heterogeneity in electrophoretic mobility. Here the relationship between phase variants of clinical isolates of S. epidermidis, icaADBC presence and electrophoretic mobility distributions is investigated. Of 105 S. epidermidis clinical isolates, 5 showed phase variation on Congo Red agar plate. Biofilm forming capability of the blackcolonies and inability of the red colonies were confirmed using a microtiter plate assay and confocal laser scanning microscopy. Upon analysis of electrophoretic mobility distributions, the black colonies displayed heterogeneity at pH 2 which was absent in the red colonies of the same strain. Surprisingly, it was shown that in all red colonies had lost the icaADBC genes. Determination of gene copy number using Real Time PCR targeting icaA showed reduction of gene copy within a culture with phase variation. In conclusion, using three fundamentally different approaches phase variation of the five clinical isolates was observed. Variants appeared through loss of icaA and icaC gens. To our knowledge this is the first report indicating S. epidermidis strains irreversible switching from biofilm + to biofilm – phenotype by deletion of ica genes. Key words: deletion, ica genes, Staphylococcus epidermidis, IcaADBC operon
The frequence of human papillomavirus (HPV) DNA on cervical cancer patients from several hospitals in Yogyakarta by PCR test. Titik Nuryastuti, Titik Nuryastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 04 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.208 KB)

Abstract

Background : Cancer of the uterine cervix (cervical cancer) is one of the most common malignant neoplasm in women in developing countries and remains a major public health problem worldwide. Several studies have proved the role of HPV as an agent STD (sexually transmitted disease), in the pathogenesis of Cervical cancer, beside the other risk factors.Objective : The aim of this study was to know the frequence of HPV infection in Yogyakarta.Material and Methods : 44 samples of Cervical cancer biopsies were collected from Sardjito General Hospital, Bethesda Hospital and private doctors, and stored frozen. DNA isolated from the biopsies of cervical cancer was amplified by PCR using consensus primer (CP I and CP IIG) from El ORF (188 bp) of HPV.Result : HPV DNA was detected in 84.1 % of the samples. In CIN I, 20 % samples were infected with HPV, 100 % in Ca epidermoid, 66.4 % in adeno Ca and 0 % in Ca adenosquamosaConclusion : The frequency of HPV in cervical Ca biopsies of certain histopathological status in Yogyakarta by PCR method was proved to be very high.Key words : HPV infection - cervical Ca - biopsy - PCR
IN VITRO EFFECT OF Chloroprocta SP. MAGGOTS SECRETION ON Staphylococcus epidermidis BIOFILM AND THE EXPRESSION LEVEL icaA OF GENE Anjarwati, Dwi Utami; Setiawati, Setiawati; Mujahidah, Mujahidah; Hapsari, Rebriarina; Nuryastuti, Titik
Indonesian Journal of Pharmacy Vol 25 No 2, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1014.224 KB) | DOI: 10.14499/indonesianjpharm25iss2pp76

Abstract

Biofilm formation and the expression of icaA gene can be induced by environment conditions that are potentially toxic for bacterial cells. The effect of green flies maggots secretion to biofilm was studied some years ago to investigate in vitro effect of secretion of Chloroprocta sp. maggots on the formation of Staphylococcus epidermidis biofilm (phenotype) and the expression level of icaA gene (genotype) for indicating its mechanism on bacterial biofilm eradication. Microtiter plate biofilm assay was used to measure the effect of Chloroprocta sp. maggots secretion at various concentration on S. epidermidis biofilm. The expression level of icaA gene was performed by Real TimePCR using lightcycler method. The biofilm susceptibility test was done against maggots excretion/secretion  using MTT assay. Whereas planktonic  susceptibility testing was carried out  using Kirby Bauer method. In the presence of maggots secretion at low concentration (5%), biofilm formation of S. epidermidis 734 was induced. In contrast, the expression level of icaA gene in  production of maggots excretion/ secretion at concentration of 5% was lower than that of without maggots secretion (1/2 Fold). Eradication of bacterial biofilm was demonstrated after 48h incubation (MD=-0,011;P<0,05), but planktonic cell. In vitro difference effect of the Chloroprocta sp. maggots secretion at low concentration to phenotype and genotype of S. epidermidis biofilm showed that the possibility of maggots secretion ability to eradicate bacterial biofilm was not mainly due to the expression level of icaA gene.
High Frequency Spontaneous Deletions within the IcaADBC Operon of Clinical Staphylococcus epidermidis Isolates. Titik Nuryastuti; Henny C. van der Mei; Henk J. Busscher; Roel Kuijer; Abu Tholib Aman; Bastian P. Krom
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.791 KB) | DOI: 10.22146/ijbiotech.7856

Abstract

Staphylococcus epidermidis has been shown to undergo a phase variation correlating with expression of the icaADBC operon which contributes to biofilm formation. Biofilm formation of Enterococcus faecalis is related to heterogeneity in electrophoretic mobility. Here the relationship between phase variants of clinical isolates of S. epidermidis, icaADBC presence and electrophoretic mobility distributions is investigated. Of 105 S. epidermidis clinical isolates, 5 showed phase variation on Congo Red agar plate. Biofilm forming capability of the blackcolonies and inability of the red colonies were confirmed using a microtiter plate assay and confocal laser scanning microscopy. Upon analysis of electrophoretic mobility distributions, the black colonies displayed heterogeneity at pH 2 which was absent in the red colonies of the same strain. Surprisingly, it was shown that in all red colonies had lost the icaADBC genes. Determination of gene copy number using Real Time PCR targeting icaA showed reduction of gene copy within a culture with phase variation. In conclusion, using three fundamentally different approaches phase variation of the five clinical isolates was observed. Variants appeared through loss of icaA and icaC gens. To our knowledge this is the first report indicating S. epidermidis strains irreversible switching from biofilm + to biofilm – phenotype by deletion of ica genes. Key words: deletion, ica genes, Staphylococcus epidermidis, IcaADBC operon
The frequence of human papillomavirus (HPV) DNA on cervical cancer patients from several hospitals in Yogyakarta by PCR test. Titik Nuryastuti Titik Nuryastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 04 (2002)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.208 KB)

Abstract

Background : Cancer of the uterine cervix (cervical cancer) is one of the most common malignant neoplasm in women in developing countries and remains a major public health problem worldwide. Several studies have proved the role of HPV as an agent STD (sexually transmitted disease), in the pathogenesis of Cervical cancer, beside the other risk factors.Objective : The aim of this study was to know the frequence of HPV infection in Yogyakarta.Material and Methods : 44 samples of Cervical cancer biopsies were collected from Sardjito General Hospital, Bethesda Hospital and private doctors, and stored frozen. DNA isolated from the biopsies of cervical cancer was amplified by PCR using consensus primer (CP I and CP IIG) from El ORF (188 bp) of HPV.Result : HPV DNA was detected in 84.1 % of the samples. In CIN I, 20 % samples were infected with HPV, 100 % in Ca epidermoid, 66.4 % in adeno Ca and 0 % in Ca adenosquamosaConclusion : The frequency of HPV in cervical Ca biopsies of certain histopathological status in Yogyakarta by PCR method was proved to be very high.Key words : HPV infection - cervical Ca - biopsy - PCR
Current in vitro assay to determine bacterial biofilm formation of clinical isolates Titik Nuryastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.978 KB) | DOI: 10.19106/JMedScie004603201406

Abstract

Biofilm mode of growth is bacterial lifestyle widespread in microbial world and represents a concernin health care. Recently, serious problem concerning biofilm-related infection is increasing significantly.However complexity of biofilm makes it difficult to conduct proper analysis. Although biofilm representsa major challenge for microbiologist, methods aimed to determine biofilm formation and developmentare not standardized yet. The aim of the present review was to provide an overview of the advantagesand disadvantages of the difference in vitro methods for determining biofilm forming ability ofclinical isolates.
Antimicrobial activity of bioactive compounds isolated from Swietenia mahagoni (L) Jacq. against Staphylococcus aureus and Pseudomonas aeruginosa Handry Darussalam,; Titik Nuryastuti; . Mursiti; . Mustofa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 04 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.331 KB) | DOI: 10.19106/JMedScie004604201402

Abstract

Widespread bacterial resistance has led to more difficult to treat infectious diseases with availableantibiotics. Therefore, new antibiotics are needed face of the growing antibiotic resistance. Swieteniamahagoni (L.) Jacq. is one of potential medicinal plants as a source new antibiotics. Five compoundshave been isolated from an ethanolic extract of S. mahagoni (L.) Jacq., however its antimicrobialactivity has not been investigated, yet. This study was conducted to evaluate the antimicrobialactivity of these compounds. Minimal Inhibitory Concentration (MIC) and Minimal BactericidalConcentration (MBC) were determined against Staphylococcus aureus and Pseudomonas aeruginosastrains. Among five compounds tested, compound 3 (3,4,5,6,7-pentaethyl-1-methoxy-1H-indazole)and compound 4 (5-ethyl-6-methoxymethyl-2-methyl-1,2-dihydropyridine) were found to be activeagainst the bactrial strains tested with the MICs and MBCs values ranged from 50 to 100 μg/mL. Inconclusion, among five compounds isolated from S. mahagoni (L.) Jacq., compound 3 and 4showed moderate antimicrobial activity against S. aureus and P. aeruginosa strains.
S. epidermidis : how to turn from commensal to be a pathogen lifestyle Titik Nuryastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 1 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1030.186 KB) | DOI: 10.19106/JMedSci005001201813

Abstract

Staphylococcus epidermidis normally is a commensal inhabitant of healthy human skin and mucosa, but also a common nosocomial pathogen in immunocompromised patients, neonates, and patients with indwelling medical devices. To distinguish the pathogen and commensal strain is a big challenge when identifying this agent with its related infection. This mini-review aims to summarize recent research in this area with a special emphasis on the virulence factor of generating genotypic and phenotypic diversity in S. epidermidis.By living between a commensal and pathogen, S. epidermidis needed to establish many strategies to face different clinical environments, including the new ecological niche of biomaterials. In addition, the growing number of immunocompromised patients increased the risk for a very sensitive host. However, further exploration of the relationship between virulence factor and in vivo pathogenesis is still needed. According to the virulence factor of these bacteria, which are considered as a real pathogen, strict control measures should be taken for S. epidermidis infection.
Current understanding of the origin, molecular biology and continuing evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Mohamad Saifudin Hakim; Luthvia Annisa; Endah Supriyati; Edwin W. Daniwijaya; Rakhmat A. Wibowo; Eggi Arguni; Titik Nuryastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 3 (2020): Special Issue: COVID-19
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (740.09 KB) | DOI: 10.19106/JMedSciSI005203202005

Abstract

Recent outbreaks of human coronaviruses, officially named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have put health authorities worldwide on a high alert. Firstly emerged in the city of Wuhan, China, SARS-CoV-2 infection is rapidly escalating into a global pandemic. It is first thought as the result of a zoonotic transmission event, similar to the previous epidemic of coronaviruses. However, a continuously increasing number of confirmed cases indicates that the virus gains capacity of efficient human-to-human transmission. Soon after the pandemic is arising, many efforts are focused on identifying the origin of SARS-CoV-2 infection in the human population. Current evidence suggests that the virus is probably derived from bat or pangolin coronaviruses as the natural host. Whether intermediate host(s) exist in the transmission cascade from bat or pangolin to humans is, to a great extent, elusive. This information is essential as the basis for infection prevention and control measures. In this review, we discuss our recent understanding of SARS-CoV-2 biology, highlighting its origin and molecular evolution.
Co-Authors . Mursiti Abu Tholib Aman Ade Christanti Putri Sidabutar Afdina Melya Ganes Febiyanti Aisyah Nur Sapriati Aisyah Nur Sapriati Altaufik Ngani Amaia, Devika N. Amalia Setyati Aman, Alia Hanifa Aminy, Sayyidah A. Anggraeni, Nuniek Annisa Aulia Savitri Annisa Somaningtyas Anton Pratama Apriyanto, Muchammad Arief Nurrochmad Atikana, Akhirta Atthobari Avanilla Fany Septyasari Bastiaan P. Krom, Bastiaan Bastian P. Krom Cicilia Widhi Astuti Cindy Elvionita Daru Estiningsih Dessy Kurnia Sari Devi Artami Susetiati Dewi Purbaningsih Dhannia Fitratiara Diah Susanti Diyan Ajeng Rossetyowati Diyan Ajeng Rossetyowati Djoko Wahyono Djoko Wahyono Dwi Utami Anjarwati Dwikisworo Setyowireni Edwin W. Daniwijaya Eggi Arguni Elya Antariksana Bachmida Emi Rusdiyati Endang Estriningsih Ervina Damayanti Estiningsih, Daru Estriningsih, Endang Firdhani Satia Primasari Fita Rahmawati Galang Ridha Allatief Hakim, Mohamad Saifudin Hamzah, Hasyrul Hananun Zharfa Hanifah Handi Virawan Hardyanto Soebono Henk J. Busscher Henk J. Busscher, Henk J. Henny C. van der Mei Henny C. van der Mei, Henny C. Hera Nirwati Ika Puspita Sari Ika Puspita Sari Ika Puspita Sari IKA PUSPITA SARI Ika Puspita Sari Ika Puspita Sari Ika Puspita Sari Ika Puspitasari Ika Puspitasari Ika Puspitasari Ika Puspitasari Ilma Tazkiya Iman Iman Ishak SKM., MPH IWAN DWIPRAHASTO Kholidah, Siti Nurhayati Kris Kurniawan Kuwat Triyana LINDA SUKMARINI Linda, Vitia Ajeng Nur Lucky Herawati Luthvia Annisa Marselinus Edwin Widyanto Daniwijaya Maulidia, Faiqoh Nur Maulidiah, Rizka Mawarti, Yuli Meilia Nhadia Amalia Mubasysyir Hasanbasri Mujahidah, Mujahidah Mukhriani, Mukhriani Munifah Wahyuddin Mursiti, Mustofa, Handry Darussalam, Titik Nuryastuti, Mursiti, Musdalifa As Mustofa Mustofa Nanang Munif Yasin Ni Luh Putu Vidya Paramita Niken Puspitasari Ning Rintiswati Nunung Yuniarti Nunung Yuniarti Nurkhalika, Rachmi Nurpagino, Bombong Prameshwara, Almarissa Ajeng Praseno Praseno Pratama, Anton Prawarni, Vidyadhari Puspa Prayoga, Muhammad Bagas Pujilestari, Dwi PUSPITA LISDIYANTI Qonita Imma Irfani Rachma Dewi Isnaini Putri Rahmawati, Fita Rakhmat Ari Wibowo Rebriarina Hapsari Retno Wahyuningrum Riandika, Andi Amelia Sari Rina Triasih Ritmaleni, Ritmaleni Rizal Fauzi Rizka Humardewayanti Asdie Rizka Humardewayanti Asdie Rizqi Nurul Khasanah Roel Kuijer Roel Kuijer, Roel SAMUEL BUDI Setiawati, Setiawati SHANTI RATNAKOMALA Subagus Wahyuono Supriyati Susi Iravati Sylvia Utami Tunjung Pratiwi Takushi Kaneko Tatang Irianti Tina Amnah Ningsih Titi Ira Pangestuti Tri Murti Andayani Tri Murti Andayani Tri Wibawa Triana Hertiani Tristina Devi Azzahra Untari, Febriana Vera Olfiana Wahyuddin, Munifah Wihda Yanuar Wihda Yanuar Yanuar, Wihda Yeli Trimayanti Yolanda Pitra Kusumadewi Yusrizal Djam’an Saleh ZULLIES IKAWATI