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All Journal Jurnal Ilmiah Farmasi Indonesian Journal of Biotechnology PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia)
Kazutaka Maeyama
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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PENGHAMBATAN PELEPASAN ENZIM -HEXOAMINIDASE DARI SEL MAST OLEH ZEORIN, SENYAWA DARI AEGLA MARMELOS CORREA Agung Endro Nugroho; Sugeng Riyanto; Mohd. Aspollah Hj. Sukari; Kazutaka Maeyama
Jurnal Ilmiah Farmasi Vol. 4 No. 2 (2007)
Publisher : Universitas Islam Indonesia

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Abstract

ABSTRACTZeorin or 6,22-Dihydroxyhopane is a compound isolated from Aegle marmelos Correacollected in Yogyakarta Indonesia. The molecular structure was confirmed in Universiti PutraMalaysia. This compound was obtained from petroleum ether extract of the leaves of Aeglemarmelos Correa. In present study, we investigated the effects of zeorin on the -hexoaminidaseenzyme release from mast cell culture. The experiment was performed by using rat basophilicleukemia (RBL-2H3) cell line, a tumor analog of mast cells. DNP24-BSA and thapsigargin were usedas immunologic and non-immunologic inducers for -hexoaminidase enzyme release from mastcells, respectively. The release of -hexoaminidase enzyme was determined by using colorimetricmethods with an enzyme substrate, p-nitrofenil-2-Acetamido-2-deoksi--D-gluko-piranosida, and amicroplate reader at 405 nm. In this study, treatment of 20 ng/mL DNP24-BSA and 0.5 Mthapsigargin could stimulate the release of -hexoaminidase enzyme from RBL-2H3 cells by25.421.62 % and 33.163.72 %, respectively. Zeorin showed potent inhibitory effects on the -hexoaminidase enzyme release, when the release induced by DNP24-BSA. In contrast, zeorin showweak inhibitory effects, when the -hexoaminidase enzyme release from RBL-2H3 cells induced bya Ca2+stimulant, thapsigargin. The IC50 values of zeorin’s effects on DNP24-BSA and thapsigarginexperiments were 33,71 M and >100 M, respectively. Based on the results, the inhibitory effect ofzeorin on the -hexoaminidase enzyme release from RBL-2H3 cells involving mechanisms relatedto the interaction of IgE on the mast cell surface or intracellular signal transductions involved inmast cell degranulation.Key words : Aegle marmelos Correa, zeorin, sel mast, -hexoaminidase enzyme
Comparison of Cytotoxic and Antiproliferative Effects of Benzylidenecyclopentanone Analogues of Curcumin on RBL-2H3 Cells Agung Endro Nugroho; S. Sardjiman; Kazutaka Maeyama
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.862 KB) | DOI: 10.22146/ijbiotech.7822

Abstract

Curcumin is a natural yellow pigment isolated from the rhizomes of Curcuma longa L. (turmeric), and has several pharmacological effects and no toxicity in both in animal and human clinical study. However, the problem of curcumin is its stability because of its active methylene moiety. Modification of this moiety to cyclopentanone is expected to increase the stability. Previous study reported that benzylidenecyclopentanone analogues of curcumin showed inhibitory effect on histamine release from RBL-2H3 (rat basophilic leukemia) cells, a tumor analog of mast cells. One of them, the hydroxy-methoxy analog (PGV-0), showed more potent effect than that of curcumin. In the present study, some benzylidenecyclopentanone analogues of curcumin were evaluated for their effects on the viability and proliferation of RBL-2H3 cells. Viable cells were counted under a light microscope with a cells-counting chamber or using the cell viability reagent WST-1. The results showed that mast cell viability and histamine content were not affected by curcumin and benzylidene cyclopentanone for 30 min incubation, however, impaired for overnight incubation. The hydroxy-dimethyl benzylidene analog (PGV-1) strongly decreased the mast cells viability for overnight incubation, and its effect was highest among the other analogues. In the proliferation study, this compound also strongly inhibited the proliferation of mast cells, whereas curcumin and hydroxy-methoxy benzylidene analog inhibited the proliferation slightly. There were no inhibitory effects on mast cells proliferation treated by dibenzylidene; dihydroxybenzylidene; and hydroxy-diethylbenzylidene cyclopentanone.Keywords : viability, proliferation, curcumin, benzylidene cyclopentanone, RBL-2H3 cells
Evaluasi Pewarnaan Alcian Blue Terhadap Sel Mast Jaringan Ikat dari Preparat Beku Jaringan Kulit Kaki Tikus Agung Endro Nugroho; Kazutaka Maeyama
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 08 No. 02 Agustus 2011
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

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Abstract

Alcian blue merupakan pewarna golongan phthalosianin tembaga yang digunakan dalam pewarnaan asam mukopolisakarida dan proteoglikan jaringan ikat. Alcian blue mengandung empat gugus tetrametillisothiouronium yang bermuatan positif (tetrakationik) yang berikat secara elektrostatik dengan muatan negatif dari glikoaminoglikan (heparin). Alcian blue tidak mewarnai semua sel mast. Hal ini kemungkinan karena sisi anionik dari heparin terlindungi oleh protein, atau ikatan kompleks dalam granul sel mast sangat kuat. Safranin sering digunakan sebagai pewarna lanjutan sebagai kombinasi alcian blue dengan safranin untuk mewarnai articular cartilage proteoglikan pada pengamatan histologi.Pewarna tunggal aclian blue terhadap sel mast jaringan ikat memberikan warna biru langit hingga hijau, sedangkan pewarna alcian blue-safranin memberikan warna biru langit hingga hijau, sedangkan pewarnaan alcian blue-safranin memberikann variasi warna yaitu biru, merah dan campuran kedua warna tersebut. Sel mast akan teramati cukup jelas pada pewarnaan toluidine blue dibandingkan dengan pewarnaan alcian blue. Namun, pada pewarnaan alcian blue terutama alcian blue-safranin, jumlah sel mast jaringan ikat yang teramati lebih banyak dibandingkan pewarnaan toluidine blue.
Co-Authors Agung Endro Nugroho Mohd. Aspollah Hj. Sukari S. Sardjiman Sugeng Riyanto