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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Farmasi Sains dan Komunitas
Kianto Atmodjo
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Optimalisasi Gula Cair dan pH Medium untuk Fermentasi Alkohol dari Jus Curucuma xanthorihiza Kianto Atmodjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 2, No 3 (2017): October 2017
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v2i3.1885

Abstract

Gula cair suatu produk baru dari gula yang tersusun dari fruktosa, glukosa dan disakarida dianggap lebih baik daripada gula kristal. Penelitian ini bertujuan untuk mengetahui konsentrasi gula cair dan pH medium yang digunakan dalam fermentasi sari temulawak (Curcuma xantorhiza Raoxb) oleh Saccharomyces cerevisiae untuk memproduksi alkohol.  Satu kg temulawak kering dihancurkan ditambah air sebanyak 8 liter, ditambahkan 250 g kecambah kacang hijau, direbus selama 30 menit, lalu didinginkan kemudian direbus lagi tiga kali rebusan disaring lalu diambil airnya kemudian dimasukkan ke dalam botol fermentasi sebanyak 300 ml kemudian ditambahkan gula cair sehingga diperoleh variasi 0,15%, 30%, 45%, 60% dan 75%. Medium sari temulawak ini diatur pH nya menjadi 4, 4,5, 5,0, 5,5 dan 6. Selanjutnya 1 g bibit S. cerevisiae ditambahkan pada tiap botol fermentasi lalu ditutup. Fermentasi berlangsung pada suhu kamar. Parameter penelitian berupa total sel, pH dan gula reduksi yang diukur tiap sehari sebanyak tiga kali, dan kadar alkohol setelah satu bulan fermentasi.  Hasil menunjukkan tidak adanya hubungan yang signifikan antara peningkatan kadar gula dan perbedaan pH medium terhadap total sel, perubahan pH dan kadar gula. Namun ada hubungan yang nyata  antara kenaikan kadar gula medium dengan alkohol yang dihasilkan. Efisiensi konversi gula menjadi alkohol tertinggi  di atas 3% terjadi pada fermentasi pada  konsentrasi gula 45%. Kadar alkohol tertinggi dihasilkan pada pH medium awal 5.
Pemanfaatan Lengkuas (Alpinia galanga L. Swartz) Untuk Mengawetkan Ikan Pindang Tongkol (Euthynnus pelamis L.) Kianto Atmodjo; Yuniarti Aida; Mursyanti Mursyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 1 (2003): February 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i1.2789

Abstract

The objective of this research was to prove that galangale rhizome (Alpinia galanga L. Swartz) can be used to preserved “pindang” tuna fish (Euthynnus pelamis L.). The fish were cooked by galangale rhizome solution (the concentration were 0, 50, 100, 150, 200 mg/l b/v) for15 minutes. Then, the fish were stored in box, three fishes /box, and stored at room temperature for 6 days. Every day, The quality of fish were measured as colour, rubberness, taste, odor, total of bacteria and fungi. The result showed that there were decreased of the fish quality, spoilaged, and many fungi and bacteri growth after two days, and the level of spoilage of fish was influenced the increasing of galangale rhizome concentration. It concluded that the galangale rhizome can not use as “pindang” tuna fish preservative.
Analisis Insektisida Organoklorin Pada Bulu Walet Sarang Putih (Collocalia fuciphaga Thunberg) Oktaf Laudensius; Pramana Yuda; Kianto Atmodjo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2003): June 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i2.2889

Abstract

The objectives of this study are to find out the kind and quantitative  of organochlorine insecticide  in  swallow (Collocalia fuciphaga Thunderberg) feathers.  The samples were plumae of wing and tail feathers from the birds were catched in Siluk, Gunungkidul and  Sedayu, Bantul on August and September 2002 The organochlorine insecticide compound were analysed by  gas chromatography-electronic catcher detector. The analysis result found out  the organochlorine insecticide in swallow feathers, were heptachlor (0-5855 ppm) and pp-DDD (0-0929 ppm).
POTENSI ANTIMIKROBIA KRIM EKSTRAK RANTING PATAH TULANG (Euphorbia tirucalli Linn.) TERHADAP Propionibacterium acnes ATCC 11827 DAN Candida albicans ATCC 24433 Melina Scandinovita Setiorini; C.J. Soegihardjo; Kianto Atmodjo
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 11, No 2 (2014)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.307 KB) | DOI: 10.24071/jpsc.00102

Abstract

Abstract: Euphorbia tirucalli L. (Euphorbiaceae) or patah tulang has been used traditionally in Java fortreatment of fungal infections (Propionibacterium acnes causing acne and Candida albicans causingcandidasis). Information on their use is available, but scientific data on their bioactivity and safety ofactions still scanty. A study was conducted on the effect of organic extracts of this plant on fungal strains.Aceton extract were evaluated through the disc diffusion assay. Aceton extract was prepared by Soxhletapparatus for eight hours. Bacteria and yeast test strains were cultured on TSA (Trypthone Soya Agar)and on PDA (Potato Dextrose Agar) for Candida albicans. A 0.5 McFarland standard suspension wasprepared. Sterile paper discs 6 mm in diameter impregnated with 10 ml of the test extract (100 mg/ml)were aseptically placed onto the surface of the inoculated media. Thymol 0,5% and ketokonazol 2% wereused as standards. Discs impregnated with dissolution medium were used as controls. Activity of theextracts was expressed according to zone of inhibition diameter. Dimethylsulfoxide (DMSO) was used forpreparing test extracts (10, 20, 40, 60, 80, 100%) and were tested for Minimum Inhibitory Concentration(MIC) to Propionibacterium acnes and Candida albicans. MIC were obtained that 100% test extract wasgreatest and 10% test extract was weakest. Then, test extracts were prepared by concentrations 10, 9, 8, 7,6, 5%, and the result were the best were 10% test extract to Propionibacterium acnes and 6% test extractto Candida albicans. Antimicrobial test for pharmaceutical preparation, i.e. cream (o/w) were preparedusing 9 and 10% test extracts mixed with cream for testing to Propionibacterium acnes and 5 and 6% testextracts with cream for testing Candida albicans.The final results were 10% test extract had MIC toPropionibacterium acnes and 6% test extract had MIC in cream to Candida albicans.Keywords: Euphorbia tirucalli L., DMSO, Thymol, antimicrobial potency, cream (o/w), Propionibacteriumacnes, Candida albicans
Co-Authors C.J. Soegihardjo Melina Scandinovita Setiorini Mursyanti Mursyanti Oktaf Laudensius Pramana Yuda Yuniarti Aida