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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
K. H. Timotius
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Pertumbuhan Enterococcus faecalis ID 6017 dan Kemampuan Dekolorisasi Beberapa Konsentrasi Orange II dalam Sistem Sinambung Patrisia Ilene Kara; V. Irene Meitiniarti; K. H. Timotius
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i2.2671

Abstract

Orange II is the most used colorant in industries which involve coloring process. So far, Orange II decolorization process by Enterococcus faecalis ID 6017 under batch system has been proven to be able to decolorize orange to colourless. In the presence of intermediates accumulation in batch system, claimed bioprocess development with continues system. An hypothesis was carried out that continues system could reduce intermediates generated by Orange II degradation. This research aimed to know the growth of Enterococcus faecalis and its Orange II decolorization ability in the medium supplemented with Orange II with different concentration under continues system. The research was set on continues system with single cultures Enterococcus faecalis, grown in medium with 80, 120, or 160 mg/L Orange II concentration. Measured parameters were Orange II concentration, biomass, glucose concentration, intermediates compound (sulphanilic acid). It could be concluded that under continues system (D = 0.06 hour-1), Enterococcus faecalis could decolorize Orange II until 160 mg/l.
Pengaruh Penyulangan Medium yang Mengandung Orange II terhadap Pertumbuhan Enterococcus faecalis ID 6017 dan Kemampuan Dekolorisasinya V. I. Meitiniarti; E. Vandiyani Sunardi; K. H. Timotius
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2006): February 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i1.2823

Abstract

The influence of fed Orange II containing medium on the growth of Enterococcus faecalis ID 6017 and its decolorization ability was studied in this research. A fed batch growth was compared with the batch growth. Total Orange II added to the both was 120 mg/L. In the fed batch, the Orange II was given into three steps. The first addition was at the initial culture medium. The second and third were added after the almost total decolorization or clearance of the Orange II given in the first or the second addition respectively. The culture was incubated under static condition and room temperature. The fed batch growth was better than the batch growth, seen from both aspects; their growth parameters and the decolorization ability. The biomass yield and specific growth rates of the fed batch was higher than the batch growth. Under fed batch growth, the decolorization was 85-94%, while the batch growth was only 54%. For achieving these performances, the fed batch growth was needed to consume more glucose.
Pertumbuhan Curah Enterococcus faecalis Id 6017 dan Kemampuan Dekolorisasi Reactive Red-2 pada Medium yang Mengandung Gliserol V. I. Meitiniarti; Morina M. Napitupulu; K. H. Timotius
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 1 (2004): February 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i1.2829

Abstract

Enterococcus faecalis ID 6017 can utilize glycerol as the source of carbon and energy for its growth. The present of glycerol in the medium containing Reactive Red-2 not only influenced its growth but also its ability to decolorize Reactive Red-2. The aim of this study was to investigate the growth of  Enterococcus faecalis (E. faecalis) and its ability to decolorize Reactive Red-2. The microbe was grown in batch system with three different growth medium, i.e. medium which contained (i) 1.643 g/l glycerol and 0.08 g/l reactive red-2, (ii) 1.643 g/l glycerol, and (iii) 0.08 g/l Reactive Red 2. The result of this study showed that the growth of  E. faecalis and its ability to decolorize Reactive Red-2 on medium contained glycerol was better than without glycerol. E. faecalis could not growth and decolorized Reactive Red-2 on medium without glycerol.
Co-Authors E. Vandiyani Sunardi Morina M. Napitupulu Patrisia Ilene Kara SATRIYAS ILYAS Yayi Suryo Prabandari