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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI
Elidar Naiola
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Karakterisasi Enzim Komersial Siklodekstrin Glukanotransferase Elidar Naiola; Nunuk Widhyastuti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v13i2.2676

Abstract

The objective of this study is to investigate the characteristic of commercial enzyme Cyclodextrin Glucanotrasferase (CGTase) from Bacillus macerans. The CGTase was purified by dialysis, gel fitration and ion exchange chromatography. Study on Characterization of the enzyme showed that the hydrolytic activity of CGTase was 480 U/mg, the optimum tempetature and pH for enzyme reaction were 450C to 550C and pH 5.0 to 8.0, respectively. The CGTase was relatively stable after heating at 550C for 10 minutes, and maintained its activity at the pH 5.0 to 9.0. The enzyme activity was inhibited by the presence of 1 mM metal ions and cause CGTase lost approximately 40% of its activity. Among the metal ions it was found that Cu2+ was the strongest inhibitor, with presence of 1mM Cu2+ the residual activity of CGTase was 24.4%. Results of purification showed that Specific activities of the enzyme during purification were 269 U/mg (crude enzyme); 955 U/mg (dialysis); 481 U/mg (gel fitrations); and 544 U/mg (ion exchange chromatography).
KARAKTERISASI DAN OPTIMASI MEDIA PRODUKSIAMILASE , DARI Aspergilus niger DAN Aspergilus clavatus Elidar Naiola
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1213

Abstract

Six fungi isolates belong to Aspergillus spp were isolated from various samples and their ability to produce amylase has been tested. It was founded that all isolate shown the amylolytic activities which shown the clear zone areas after pouring with iodium solution. Two isolates which name Aspergillus niger (ISO 482) dan A. clavatus (ISO 468) to be the most active compare to another. The amylase activity of two isolate was studied in media contain rice and rice brain as a carbohydrate sources. Based on lower cost and easy to reach rice brain (local waste agriculture product) were choose as the alternative media to produce enzyme amylase from Aspergillus niger (ISO 482). The activities 2 of enzyme obtain was 54,14 x 10  U/ml (one unit activity is define as micromoles of glucose produce per ml per minute). The optimiation was done at room temperature for 7 days. The result showed the activity of enzyme increase during the fermentation process, at the first 2 2 day activity was 18,77 x 10 U/ml and reached the maximum activity (91,64 x 10 U/ml) after 3 days. The optimum temperature for enzyme reaction was 40 - 50 °C, optimum pH was (pH 5.0 - 7.0) and enzyme was relatively stable under such conditions.
SELEKSIBIAK Aspergillus spp. PENGHASIL AMILASE UNTUK PEMBUATAN PROTEIN SEL TUNGGAL DARITEPUNG GANYONG (Canna edulis Kerr.) Elidar Naiola
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1266

Abstract

Two strains of fungi were used as innculant to produce Single Cell Protein (SCP) using the ganyong (Canna edulis Kerr.)starch as carbohydrate sources. Those two strains were Aspereillus nieer and A.clavatus identified as amylolytic fungi wich produce hight clear zone to colony diameter ratio in medium containing soluble starch with Iodium test.At the suitable interval time (0, 24, 48, 72 and 96 hours) the dry cell weight or biomass, pH and reducing sugar concentration in the medium containing ganyong starch was determined.It was found that maximum amount of reducing sugar concentration was hisher in A. clavatus (8.5 e/l) compared to A. nieer (4,5 g/l), were produced after 24 hours.The maximum amount of biomass produced by A. nieer was 8,5 g/l after 48 hr, while by A clavatus was 7,25g/l after 96 hr.
ISOLASI, SELEKSI DAN OPTTMASI PRODUKSI PROTEASE DARIBEBERAPAISOLAT BAKTERI Elidar Naiola; Nunuk Widhyastuti
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1219

Abstract

Thirty-seven out of sixty-one bacterial isolates from various sources of samples were screened for protease production. The isolate of ISO PL3 could produce the highest enzyme activity, and it was used as a standard bacterial strain in this observation. For any reason,we implemented ISO PL2 to study the optimum condition for producing bacterial protease. Result shows that the maximum protease activity was obtained in a medium containing 100 gram of rice brand in a liter tofu liquid waste. The optimum for incubation was 4- 6 days (agitation of 130 rpm at room temperature) and pH 5.0 - 6.0. After cultivating on this liquid medium, the maximum protease activity of the 2 ISO PL3 was 113,52 x 10' U/ml. From the studies on morphological and physiological characterization, it was indicated that ISO PL3 resemble with the species Bacillus macerans.
KARAKTERISASI AKTIVITAS ANTIMIKROBA BEBERAPA ASAM LEMAK ASKORBIL Elidar Naiola
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1249

Abstract

The study is directed t investigate the antimicrobial activity f six ascorbyl fatty acids. The minimum concentrations were observed in ascorbyl caprate and ascorbyl laurate. Ascorbyl caprate was the strongest ester with the minimum inhibitory concentration (1,25-5 nM). Among microorganisms tested,S. cerevisiae was more senseitive than others. The sensitivity of S. cerevisiae was depending on the strains and S.cerevisiae OUT7054 was the most sensitive strain to the ascorbyl caprate which was the sterilizing agent rather than microbiostic agent.The antimicrobial activity of ascorbyl caprate was infuenced remarkably by temperature and pH.The most effective conditbns for sierilizatin of yeast were higher temperature and low pH.
Co-Authors Nunuk Widhyastuti