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AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL BUAH PAPRIKA HIJAU (Capsicum annum L.) Warsi Warsi; Any Guntarti
Pharmaciana Vol 3, No 1 (2013): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.822 KB) | DOI: 10.12928/pharmaciana.v3i1.415

Abstract

Green paprica (Capsicum annum L.) is one of fruits which have contain–carotene, vitamin E and vitamin C, which have antioxidant activity. The aim of thisresearch is to know an antioxidant capacity of green paprica. An analysis of theantioxidant activity of this research used radical scavenging DPPH(1,1–difenil–2–pikrilhidrazil) method. The sample was preparated with maceration for4 days using methanol as the solvent. Crude extract was evaluated qualitatively using0,4 mM DPPH reagent. This result of qualitative analysis showed that green papricahave an antioxidant activity. Then, absorbance was measured using UV–Visspectrophotometer at wavelength of 517 nm. The result of this analysis obtained EC50value of green paprica was 0,3399 ± 0,01408 mg/ml.
UJI AKTIVITAS ANTIOKSIDAN FRAKSI ETER HASIL HIDROLISIS INFUSA DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) DENGAN METODE DPPH (1,1-DIPHENIL-2-PICRYLHYDRAZYL) Agustina Ardianti; Any Guntarti; Zainab Zainab
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.223 KB) | DOI: 10.12928/pharmaciana.v4i1.391

Abstract

Antioxidant is compounds which have abilities to inhibit oxidation rate. Anredera cordifoliafolium consist of flavonoid which have activities as antioxidant. The purpose of this research was toknow antioxidant activity at ether fraction of hydrolysed aquous extract of Binahong folium (Anrederacordifolia). Ether fraction was obtained by hydrolysing the infuse Binahong folium (Anrederacordifolia) with HCl 2N then fractionated with ether and then evaporated. The antioxidant activity wasmeasured by DPPH method using spectrophotometer. The maximum wavelength and operating timequercetin and samples were 515,0 nm (quersetin) and 514,0 nm (ether fraction), 19-30 minute(quersetin) and 19-29 minute (ether fraction). Scavenging activity test done with the DPPH method(1,1- dyphenyl-2-picrylhydrazyl). The decreasing of DPPH radical solution decreased the absorbantintensity. The percentage of radical scavenging activity was performed as EC50 (EffectiveConcentration). The results indicated that both samples showed free radical scavenging activity withEC50 value for quercetin (2,702±0,15) µg/ml, and ether fraction of (249,31±9,26) µg/ml. Statisticalanalysis using Kruskall Wallis method with 95% of confident level followed by Mann Whitney testgiving evidence the results between samples and positive control (quercetin) are significantlydifference.
PENETAPAN KADAR Pb PADA SHAMPOO BERBAGAI MERK DENGAN METODE SPEKTROFOTOMETRI SERAPAN ATOM Farida Jaya; Any Guntarti; Zainul Kamal
Pharmaciana Vol 3, No 2 (2013): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.392 KB) | DOI: 10.12928/pharmaciana.v3i2.425

Abstract

The source of lead pollution (Pb) can come from cosmetic product including shampoo. The purpose of thisresearch are to know wether there is Pb in the shampoo cosmetic based on different brands and colours of theshampoo. The sample of the shampoos that used in this research are A, B, C, and D. Each of the samples replicated5 times, then analyzed with atomic absorbtion specktrophotometry. Then, one of the sample are taken from onebrand with different colour and replicated 5 times. The result of this research shows that from the different brandof shampoos contain Pb. The average level of Pb that obtained from analysis are, brand A (0,4838 ppm 0,0538ppm), brand B (0,6935 ppm 0,3131 ppm), brand C (0,8354 ppm 0,0405 ppm), and brand D ( 0,9517 ppm0,0811 ppm). Whereas for the same shampoo brand but different in colour had average level of Pb: shampoo I (redcolour)= (0,5428 0,0192) ppm, Shampoo II (yellow colour) = (0,3606 0,0255) ppm, Shampoo III (blue colour)= (0,4385 0,0062) ppm, and Shampoo IV (green colour) = (0,1427 0,0133) ppm.