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Magdalena E.F Kolopita
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Environmental Science

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Kondisi Lingkungan Perairan Pada Lahan Budidaya Rumput Laut Kappaphycus alvarezii Di Desa Jayakarsa Kabupaten Minahasa Utara Mudeng, Joppy D; Kolopita, Magdalena E.F; Rahman, Abdul
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6953

Abstract

A research had been conducted to evaluate seaweed and waters condition at seaweed  culture area at Jayakarsa Village.  Research locations were divided into three station: 1) at former culture area near mangrove area and the beach, 2)  at active culture area, 3) at former culture area with sandy bottom and ”lamun” field.  Water quality measured included temperature, salinity, pH, DO, turbidity, flow rate, water depth, bottom type. Protectivenss, risk, and accesibility were also observed. Data was analyzed descriptively and then compared to standard value established in Kangkan (2006).  Water condition at station 1 and 2 was categorized Grade 2 (moderately suitable) while at station 3 was marginally suitable which was categorized Grade 3. Major problems faced by the farmers was ice-ice disease, silt and epiphytes that could reduce the production.   Keywords: Kappaphycus alvarezii, water quality, Jayakarsa Village, moderately suitable, marginally suitable
Respon imun krustase Manoppo, Henky; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.2.2014.4904

Abstract

Crustacean does not have adaptive immune system and mostly depends on innate or nonspecific immune response. This system can recognize and destroy foreign materials including pathogen.  The nonspecific immune responses of crustacean consist of cellular and humoral responses.  In cellular response, hemocyte plays an important role in body defenses to pathogen including virus, bacteria, fungi, and parasites. In humoral response, recognition of pathogen is mediated by protein and enzyme such as β-1,3-glucan-binding protein (BGBP),  lipopolysaccharide-binding protein (LPS-BP), peptidoglycan recognition protein, phenoloxydase enzime. Keywords: crustacean, nonspecific immune response, cellular responses, humoral response, protein, enzime
Diagnosa Penyakit Bakterial Pada Ikan Nila (Oreocrhomis niloticus) Yang Di Budi Daya Pada Jaring Tancap Di Danau Tondano Ashari, Chairanitansyah; Tumbol, Reiny A; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.3.2014.5700

Abstract

This research aimed to diagnose bacterial diseases on nile tilapia cultured in stick net cage in Lake Tondano. Fish samples were taken from three locations of net cages in Lake Tondano namely Paleloan, Eris and Remboken villages. Three samples of fish were taken from each location/village. The samples taken showed clinical symptoms of bacterial infection such as hemorrhagic on the abdomen, the base of the fin, anal and chest, irregular swimming fish, hemorrhagic on the gills, fish refuse food, remain silent on the outskirts of net cage. Bacteria were isolated by using a sterile loop from head kidney (anterior kidney), which is located on the front, below the spine near the head. Bacterial cultures were performed on TSA media by making a zigzag scratches. The petri dish containing bacterial isolates were subsequently incubated in an incubator at a temperature of 35oC for 24 hours. The water quality parameter data measured consisted of temperature, Dissolved Oxygen, pH, ammonia, brightness. The results of the study showed that farmed tilapia in Lake Tondano has been infected with Aeromonas hydrophila that causes MAS (Motile Aeromonad Septicemia). The water quality, both chemical and physics around the net cages in the Lake Tondano was still in good condition and suitable for fish cultivation.   Keywords: Oreocrhomis niloticus, Bacterial disease, septicemia aeromonad motile, fixed net cage, Lake Tondano
Identifikasi Parasit pada Ikan Kerapu Sunu, Plectropomus leopardus Sauyai, Kristian; Longdong, Sammy N.J; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.3.2014.5707

Abstract

This research aimed to identify, analyze and determine the prevalence rate and dominance index of parasites on grouper (Plectropomus leopardus) landed at Bahu Beach. The number of samples used was 20 fishes measuring 230,68-490,10 g in weight and 24-29 cm in length. Ectoparasites were observer in mucus, fin, and gill while endoparasites were observed in peritoneal cavity and intestine of fish. Parasites invested grouper were Lernaeocera branchialis, Unitubulotestis sardae Acanthochondria cornuta, Mirasidium Schistosomatium douthiti, Argulus sp and  Gnathia maxillaris. All the parasites were found in fish gill and only one species in the mucus. Prevalence rate of parasites was 40%.  Based on parasite species, prevalence rate of  Unitubulotestis sardae was 20%, Acanthochondria cornuta 10%, Gnathia maxillaries 10%, Lernaeocera branchilia 5%, Mirasidium Schistosomatium douthiti 5% and Argulus sp 5%.  Parasites infected grouper dominated by three species namely Unitubulotestis sardae, Acanthochondria cornuta, and Gnathia maxillaris in which all the species were dominant in the gill.   Keywords :  Parasite, Plectropomus leopardus, prevalence rate, dominance index
Penambahan bakasang pada pakan benih sidat (Anguilla marmorata) untuk meningkatkan sistem imun non spesifik Pinoke, Steven A.J; Tumbol, Reiny A; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 3, No 3 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.3.2015.10361

Abstract

This study was aimed to examine the effect of immunostimulant bakasang (fermented product from fish) and to determine the optimal dose of bakasang in increasing the non-specific immune response of eels. The eels were obtained from Institute of Freshwater Aquaculture (BPBAT) Tatelu. Bakasang as immunostimulant ingredient contained Lactic Acid Bacteria (LAB) with the bacteria concentration of 105 CFU/ml was prepared by diluting with clean water to get the doses desired. The feed was subsequently mixed with the commercial fish feed in powder form and mixed well with water to form paste. This research used Two-ways ANOVA with 4 different types of treatments A (0 ml/kg feed),   B (50 ml/kg feed), C (100 ml/kg feed), D (150 ml/kg feed) and 3 repetitions. The fish were feed with treatments diets for 3 weeks with a dose of 3% body weight per day with a frequency of twice a day (at 08:00 am) and the afternoon (16.00 pm). The parameters measured was Total Leukocyte Count (TLC) which was measured every week (t0, t1, t2, t3). The results indicated that the addition of bakasang on eels’feed gave a significant increased on TLC, and that the highest TLC was found in the dose of 100 ml/kg feed. This shows that the optimal dose of bakasang in increasing the TLC of eels  was 100 ml/kg feed after administration of 3 weeks. Keywords: Eels, Bakasang, Total Leucocytes Count
Co-Authors Aminuyati Chairanitansyah Ashari Henky Manoppo Joppy Mudeng Kristian Sauyai Reiny A Tumbol Sammy N.J Longdong Steven A.J Pinoke, Steven A.J