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All Journal Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
Komar Sumantadinata
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AKUMULASI LOGAM BERAT DAN PENGARUHNYA TERHADAP SPERMATOGENESIS KERANG HIJAU (Perna viridis) Jalius ,; D. Djoko Setiyanto; Komar Sumantadinata; Etty Riani; Yunizar Ernawati
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 15 No. 1 (2008): Juni 2008
Publisher : Institut Pertanian Bogor

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Abstract

Perairan Teluk Jakarta, Banten, dan Lada telah mengalami pencemaran, terutama tercemar oleh logam berat seperti Pb, Cd, Cr dan Hg. Penelitian ini bertujuan untuk mengetahui kandungan logam tersebut dalam gonad kerang hijau (Perna viridis) dan pengaruh akumulasi logam tersebut pada spermatogenesis kerang hijau. Parameter yang diamati adalah jumlah spermatogenium, spermatosit primer, spermatosit sekunder, spermatozoa, total se-sel kelamin, diameter, luas dan volume lumen folikel gonad kerang hijau, pada tingkat IV spermatogenesis. Hasil penelitian menunjukan bahwa gonad kerang hijau yang berasal dari Teluk Jakarta telah terakumulasi logam berat Pb = 359.75±272.41 ppb; Cd = 36.559±21.90 ppb; Cr = 504.21±448.64 ppb dan Hg = 0.0092± 0.0085 ppb. Logam Cd (13.13 ppb) dan Pb (0.077 ppm) ditemukan dalam gonad kerang hijau yang berasal dari Teluk Banten, tetapi tidak terdeteksi logam Cr dan Hg. Namun tidak ditemukan logam berat tersebut dalam gonad kerang hijau berasal dari Teluk Lada. Di Teluk Jakarta ditemukan korelasi antara kandungan logam Cd terhadap perkembangan jumlah sel spermatozoa (r = 0.64), total sel kelamin (r = 0.60, diameter (r = 0.57), luas (r = 0.71) and volume (r = 0.71) lumen folikel gonad. Logam Hg berpengaruh terhadap perkembangan spermatogenium (r = 0.68) dan spermatosit sekunder (r = 0.61), sedangkan logam Cr mempunyai pengaruh terhadap luas (r = 0.75) dan volume (r = 0.75) lumen folikel gonad.Kata kunci: akumulasi, logam berat, spermatogenesis dan kerang hijau.
Donor preparation for germ cell transplantation in giant gouramy: the viability of spermatogonia isolated from giant gouramy cold preserved testis Irma Andriani; Ita Djuwita; . Alimuddin; Komar Sumantadinata
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The recent study has been conducted to develop testicular germ cell (TGC) transplantation as a tool for preservation and propagation of male germ-plasm from endangered fish species. In practice of TGC transplantation,recipient and donor cell may not be immediately available at the same time whereas the testis can not be survive longer when it is outside of the body. Therefore, preservation of testis tissue may be required before transplantation.The research was conducted to evaluate the viability of spermatogonia isolated from short term preserved testis. Testis was preserved in physiological NaCl solution at 4 oC for 6, 12, 24 and 48 hours. Testis were dissociated in 0.5% trypsin and 3% DNase 10 IU/8L in PBS (phosphate buffered solution) complemented with 5% FBS ( fetal bovine serum), 25 mM HEPES and 1mM CaCl2 to obtain testicular germ cell suspension. The testicular germ cells isolated from 24 and 48 hours preservation were performed in trypan blue staining dye (1:1) and the viability of spermatogonia were observed under microscope. The results showed that the viability of spermatogonia started todecrease significantly in 12 hours preservation (P0.05) and up to 48 hours preservation, cell viability was as high as 54,48±8,33%. In conclusion, preserved testicular tissue at 4oC still produced viable spermatogonia that areallowed to use as the source of donor cell for testicular germ cell transplantation of giant gourami.
Co-Authors Alimuddin D. Djoko Setiyanto Etty Riani Irma Andriani Ita Djuwita Jalius , Yunizar Ernawati